4E-BP1 (phospho Thr37/Thr46) antibody

Wild-type (WT) and 4E-BP1 (phospho Thr37/Thr46) knockout (KO) HeLa cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with 4E-BP1 (phospho Thr37/Thr46) antibody (GTX133181) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Untreated (–) and treated (+) C2C12 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membranes were blotted with 4E-BP1 (phospho Thr37/Thr46) antibody (GTX133181) diluted at 1:1000 and 4E-BP1 antibody [N1C3] (GTX109162) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Untreated (–) and treated (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membranes were blotted with 4E-BP1 (phospho Thr37/Thr46) antibody (GTX133181) diluted at 1:3000 and 4E-BP1 antibody [N1C3] (GTX109162) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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Untreated (–) and treated (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with 4E-BP1 (phospho Thr37/Thr46) antibody (GTX133181) diluted at 1:1000.

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with 4E-BP1 (phospho Thr37/Thr46) antibody (GTX133181) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with 4E-BP1 (phospho Thr37/Thr46) antibody (GTX133181) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with 4E-BP1 (phospho Thr37/Thr46) antibody (GTX133181) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.