GeneTex

  • Country / Location Selection

United States (US)

4E-BP1 (phospho Thr37/Thr46) antibody

KO/KD Orthogonal
Anti-4E-BP1 (phospho Thr37/Thr46) antibody used in Western Blot (WB). GTX133182
Anti-4E-BP1 (phospho Thr37/Thr46) antibody used in Western Blot (WB). GTX133182
Anti-4E-BP1 (phospho Thr37/Thr46) antibody used in Western Blot (WB). GTX133182
Anti-4E-BP1 (phospho Thr37/Thr46) antibody used in Western Blot (WB). GTX133182
Anti-4E-BP1 (phospho Thr37/Thr46) antibody used in Western Blot (WB). GTX133182

Cat. No. GTX133182

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse, Rat, Drosophila
Package
100 μl ($399),
25 μl ($169)
See all 4E-BP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
Not tested in other applications.

Calculated MW

13 kDa. ( Note )

Positive Control

293T (starvation for 24 hr and 20%FBS treatment for 0.5 hr) , HeLa , Neuro2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat2

Predict Reactivity

Zebrafish, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.12 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Thr37/Thr46 of human 4E-BP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886857

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

eukaryotic translation initiation factor 4E binding protein 1 , 4E-BP1 , 4EBP1 , BP-1 , PHAS-I

Background

This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-4E-BP1 (phospho Thr37/Thr46) antibody used in Western Blot (WB). GTX133182

GTX133182 WB Image

Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with 4E-BP1 (phospho Thr37/Thr46) antibody (GTX133182) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Drosophila Thor is the homolog of mammalian 4E-BP1. (Gene ID: 33569/ UniProt: Q9XZ56)

Anti-4E-BP1 (phospho Thr37/Thr46) antibody used in Western Blot (WB). GTX133182

GTX133182 WB Image

KO/KD

Wild-type (WT) and 4E-BP1 (phospho Thr37/Thr46) knockout (KO) HeLa cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with 4E-BP1 (phospho Thr37/Thr46) antibody (GTX133182) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-4E-BP1 (phospho Thr37/Thr46) antibody used in Western Blot (WB). GTX133182

GTX133182 WB Image

Orthogonal

Untreated (–) and treated (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with 4E-BP1 (phospho Thr37/Thr46) antibody (GTX133182) diluted at 1:1000.

Anti-4E-BP1 (phospho Thr37/Thr46) antibody used in Western Blot (WB). GTX133182

GTX133182 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with 4E-BP1 (phospho Thr37/Thr46) antibody (GTX133182) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-4E-BP1 (phospho Thr37/Thr46) antibody used in Western Blot (WB). GTX133182

GTX133182 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with 4E-BP1 (phospho Thr37/Thr46) antibody (GTX133182) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Yang M et al. J Cell Mol Med 2021; 25 (21) : 10291-10305 Targeting of EIF4EBP1 by miR-99a-3p affects the functions of B lymphocytes via autophagy and aggravates SLE disease progression.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

There are currently no reviews for 4E-BP1 (phospho Thr37/Thr46) antibody (GTX133182). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169