GeneTex
United States (US)

4E-BP1 (phospho Thr37) antibody

Anti-4E-BP1 (phospho Thr37) antibody used in Western Blot (WB). GTX32322
Anti-4E-BP1 (phospho Thr37) antibody used in IHC (Paraffin sections) (IHC-P). GTX32322

Cat No. GTX32322

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:1000
IHC-P 1:100 - 1:200
Not tested in other applications.

Calculated MW

13 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

0.42% Potassium phosphate, 0.87% Sodium chloride pH 7.3, 30% glycerol, 0.01% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of 4E-BP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Eukaryotic Translation Initiation Factor 4E Binding Protein 1,4E-Bp1,4Ebp1,Bp-1,Phas-I,Eif4Ebp1

Background

This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Anti-4E-BP1 (phospho Thr37) antibody used in Western Blot (WB). GTX32322

GTX32322 WB Image

WB analysis of insulin-treated HEK293T (A), insulin-treated NIH3T3 (B), insulin-treated PC12 (C) whole cell lysates using GTX32322 4E-BP1 (phospho Thr37) antibody.

Anti-4E-BP1 (phospho Thr37) antibody used in IHC (Paraffin sections) (IHC-P). GTX32322

GTX32322 IHC-P Image

IHC-P analysis of formalin fixed human breast cancer tissue section using GTX32322 4E-BP1 (phospho Thr37) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

Package List Price ($)
$ 319