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4E-BP1 (phospho Thr45) antibody

Anti-4E-BP1 (phospho Thr45) antibody used in Western Blot (WB). GTX50259
Anti-4E-BP1 (phospho Thr45) antibody used in IHC (Paraffin sections) (IHC-P). GTX50259

Cat No. GTX50259

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Honeybee
Package
100 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

13 kDa. ( Note )

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of threonine 45 (S-T-T(p)-P-G) derived from human 4E-BP1.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

eukaryotic translation initiation factor 4E binding protein 1 , 4E-BP1 , 4EBP1 , BP-1 , PHAS-I

Background

This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-4E-BP1 (phospho Thr45) antibody used in Western Blot (WB). GTX50259

GTX50259 WB Image

WB analysis of extracts from HeLa cells using GTX50259 4E-BP1 (phospho Thr45) antibody with or without blocking peptide.

Anti-4E-BP1 (phospho Thr45) antibody used in IHC (Paraffin sections) (IHC-P). GTX50259

GTX50259 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50259 4E-BP1 (phospho Thr45) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf