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4E-BP1 (phospho Thr45) antibody

Orthogonal
Anti-4E-BP1 (phospho Thr45) antibody used in Western Blot (WB). GTX50259
Anti-4E-BP1 (phospho Thr45) antibody used in IHC (Paraffin sections) (IHC-P). GTX50259

Cat. No. GTX50259

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Honeybee
Package
100 μl ($399)
See all 4E-BP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

13 kDa. ( Note )

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of threonine 45 (S-T-T(p)-P-G) derived from human 4E-BP1.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

RRID

AB_11175932

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

eukaryotic translation initiation factor 4E binding protein 1 , 4E-BP1 , 4EBP1 , BP-1 , PHAS-I

Background

This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-4E-BP1 (phospho Thr45) antibody used in Western Blot (WB). GTX50259

GTX50259 WB Image

Orthogonal

WB analysis of extracts from HeLa cells using GTX50259 4E-BP1 (phospho Thr45) antibody with or without blocking peptide.

Anti-4E-BP1 (phospho Thr45) antibody used in IHC (Paraffin sections) (IHC-P). GTX50259

GTX50259 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50259 4E-BP1 (phospho Thr45) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

REFERENCE

Application Reference

Lu CY et al. Arch Insect Biochem Physiol 2018; : e21468 Cellular energy metabolism maintains young status in old queen honey bees (Apis mellifera).
Application : WB
Reactivity : Honeybee
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399