5-Carboxylcytosine / 5-caC antibody

ICC/IF analysis of 293T cells overexpressing either the mouse FLAG-tagged wild-type Tet1 (Tet1 CD) or the catalytically inactive FLAG-tagged C-terminal domain of Tet1 (Tet1 mCD) using GTX60801 5-Carboxylcytosine / 5-caC antibody.
Dilution : 1:500

Dot blot analysis of 25 and 125 ng of the synthetic oligonucleotides containing different modified C-bases (indicated in red) using GTX60801 5-Carboxylcytosine / 5-caC antibody.
Dilution : 1:1,000

ChIP analysis of 2 μg of J1 ES genomic DNA spiked with 1 pg of a control DNA fragment (approximately 700 bp from the RFP (Ring finger protein) gene) containing different cytosine modifications using GTX60801 5-Carboxylcytosine / 5-caC antibody. The mC and hmC control DNA was generated by PCR with the corresponding nucleotide. The caC control fragment was obtained by in vitro methylation using M.SssI methyltransferase followed by oxidation with purified Tet2. The IP'd DNA was subsequently anaysed by qPCR using primers specific for the control DNA fragments and for GAPDH, used as a negative control.