5-HT1A receptor antibody [HL3657]

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with HTR1A antibody (GTX641648) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.

Boiled and unboiled whole cell extract (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with 5-HT1A receptor antibody [HL3657] (GTX641648) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data are based on Human Protein Atlas program.

Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with 5-HT1A receptor antibody [HL3657] (GTX641648) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

HTR1A antibody (GTX641648) detects HTR1A protein by flow cytometry analysis.
Sample: Non-transfected and transfected 293T cells were fixed in 4% paraformaldehyde at 4ºC for 15 min and washed with 0.1% saponin buffer.
The cells were following stained with HTR1A antibody [HL2850] (GTX640121) diluted at 1:50 or a Rabbit IgG isotype control (GTX35035) control at 4ºC for 1 hour.