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5-HT1A receptor antibody [N3C1], Internal

Anti-5-HT1A receptor antibody [N3C1], Internal used in Western Blot (WB). GTX104703
Anti-5-HT1A receptor antibody [N3C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104703
Anti-5-HT1A receptor antibody [N3C1], Internal used in IHC (Frozen sections) (IHC-Fr). GTX104703
Anti-5-HT1A receptor antibody [N3C1], Internal used in Western Blot (WB). GTX104703
Anti-5-HT1A receptor antibody [N3C1], Internal used in Western Blot (WB). GTX104703
Anti-5-HT1A receptor antibody [N3C1], Internal used in Western Blot (WB). GTX104703

Cat. No. GTX104703

Reference 14
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all 5-HT1A receptor products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Predict Reactivity

Rabbit, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.03 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human 5-HT1A receptor. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1241307

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

5-hydroxytryptamine receptor 1A , 5-HT-1A , 5-HT1A , 5HT1a , ADRB2RL1 , ADRBRL1 , G-21 , PFMCD

Cellular Localization

Cell membrane

Database

Research Area

DATA IMAGES

Anti-5-HT1A receptor antibody [N3C1], Internal used in Western Blot (WB). GTX104703

GTX104703 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with 5-HT1A receptor antibody [N3C1], Internal (GTX104703) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-5-HT1A receptor antibody [N3C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104703

GTX104703 ICC/IF Image

5-HT1A receptor antibody [N3C1], Internal detects 5-HT1A receptor protein by immunofluorescent analysis.
Sample: DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: 5-HT1A receptor protein stained by 5-HT1A receptor antibody [N3C1], Internal (GTX104703) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-5-HT1A receptor antibody [N3C1], Internal used in IHC (Frozen sections) (IHC-Fr). GTX104703

GTX104703 IHC-Fr Image

5-HT1A receptor antibody [N3C1], Internal detects 5-HT1A receptor protein by immunohistochemical analysis.
Samples: Frozen Sectioned adult mouse hippocampus.
Green: 5-HT1A receptor protein stained by 5-HT1A receptor antibody [N3C1], Internal (GTX104703) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 10 min

Anti-5-HT1A receptor antibody [N3C1], Internal used in Western Blot (WB). GTX104703

GTX104703 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with 5-HT1A receptor antibody [N3C1], Internal (GTX104703) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-5-HT1A receptor antibody [N3C1], Internal used in Western Blot (WB). GTX104703

GTX104703 WB Image

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with 5-HT1A receptor antibody [N3C1], Internal (GTX104703) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-5-HT1A receptor antibody [N3C1], Internal used in Western Blot (WB). GTX104703

GTX104703 WB Image

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with 5-HT1A receptor antibody [N3C1], Internal (GTX104703) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Kim HJ et al. Front Cell Neurosci 2023; 17 : 1162966 Possible involvement of microglial P2RY12 and peripheral IL-10 in postpartum depression.
Lim J et al. Front Pharmacol 2022; 13 : 1062650 Differentiated HT22 cells as a novel model for in vitro screening of serotonin reuptake inhibitors.
Application : WB, ICC/IF
Reactivity : Mouse
Saurav Bhowmick et al. eNeuro 2021; 8 (4) Intercellular Adhesion Molecule-1-Induced Posttraumatic Brain Injury Neuropathology in the Prefrontal Cortex and Hippocampus Leads to Sensorimotor Function Deficits and Psychological Stress.
Application : WB
Reactivity : Mouse
2021; : e12771 Melatonin pretreatment alleviates the long-term synaptic toxicity and dysmyelination induced by neonatal Sevoflurane exposure via MT1 receptor-mediated Wnt signaling modulation.
Vegas-Su?rez S et al. Br J Pharmacol 2021; 178 (12) : 2516-2532 The effect of 5-HT1A receptor agonists on the entopeduncular nucleus is modified in 6-hydroxydopamine-lesioned rats.
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REVIEW

5-HT1A receptor antibody [N3C1], Internal Cat. No. GTX104703

Rating
( Average 5 based on 1 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-5-HT1A receptor antibody [N3C1], Internal used in Western Blot (WB). GTX104703
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Front Behav Neurosci in 2015.PMID: 25870546
Submit a Review
Application Reference
Kim HJ et al. Front Cell Neurosci 2023; 17:1162966 Possible involvement of microglial P2RY12 and peripheral IL-10 in postpartum depression.
Lim J et al. Front Pharmacol 2022; 13:1062650 Differentiated HT22 cells as a novel model for in vitro screening of serotonin reuptake inhibitors.
Application : WB, ICC/IF
Reactivity : Mouse
Saurav Bhowmick et al. eNeuro 2021; 8 (4) Intercellular Adhesion Molecule-1-Induced Posttraumatic Brain Injury Neuropathology in the Prefrontal Cortex and Hippocampus Leads to Sensorimotor Function Deficits and Psychological Stress.
Application : WB
Reactivity : Mouse
2021;:e12771 Melatonin pretreatment alleviates the long-term synaptic toxicity and dysmyelination induced by neonatal Sevoflurane exposure via MT1 receptor-mediated Wnt signaling modulation.
Vegas-Su?rez S et al. Br J Pharmacol 2021; 178 (12):2516-2532 The effect of 5-HT1A receptor agonists on the entopeduncular nucleus is modified in 6-hydroxydopamine-lesioned rats.
Chen L et al. J Int Med Res 2020; 48 (5):3.00061E+14 Electroacupuncture improves synaptic plasticity by regulating the 5-HT1A receptor in hippocampus of rats with chronic unpredictable mild stress.
Kim J et al. Biomol Ther (Seoul) 2020; 28 (3):282-291 Toluene Inhalation Causes Early Anxiety and Delayed Depression with Regulation of Dopamine Turnover, 5-HT1A Receptor, and Adult Neurogenesis in Mice.
Sugimoto K et al. Int J Mol Sci 2020; 22 (1) Serotonin/5-HT1A Signaling in the Neurovascular Unit Regulates Endothelial CLDN5 Expression.
Vegas-Su?rez S et al. Br J Pharmacol 2020; 6-Hydroxydopamine lesion and levodopa treatment modify the effect of buspirone in the substantia nigra pars reticulata.
Reactivity : Rat
Rudyk C et al. J Neuroinflammation 2019; 16 (1):120 Leucine-rich repeat kinase-2 (LRRK2) modulates paraquat-induced inflammatory sickness and stress phenotype.
Application : WB
Reactivity : Mouse
Lim J et al. J Neurosci 2018; (Epub) LRRK2 G2019S induces anxiety/depression-like behavior prior to the onset of motor dysfunction with 5-HT1A receptor upregulation in mice.
Huang F et al. Front Behav Neurosci 2015; 9:70 Deletion of mouse FXR gene disturbs multiple neurotransmitter systems and alters neurobehavior.
Nakamura Y et al. Biotechnol Bioeng 2015; Applications of yeast-based signaling sensor for characterization of antagonist and analysis of site-directed mutants of the human serotonin 1A receptor.
Application : WB
Reactivity : Human
Lin YL et al. Brain Behav Immun 2012; 26 (3):459-68 Prenatal lipopolysaccharide exposure increases anxiety-like behaviors and enhances stress-induced corticosterone responses in adult rats.
Application : WB
Reactivity : Rat
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169