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5-HT1A receptor antibody [N3C1], Internal

Anti-5-HT1A receptor antibody [N3C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104703
Anti-5-HT1A receptor antibody [N3C1], Internal used in IHC (Frozen sections) (IHC-Fr). GTX104703
Anti-5-HT1A receptor antibody [N3C1], Internal used in Western Blot (WB). GTX104703
Anti-5-HT1A receptor antibody [N3C1], Internal used in Western Blot (WB). GTX104703

Cat No. GTX104703

Reference 11
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)
See all 5-HT1A receptor products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Positive Control

Molt-4 , Raji , D-hippocampus C9

Predict Reactivity

Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS,20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.97 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human 5-HT1A receptor. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1241307

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

5-hydroxytryptamine receptor 1A , 5-HT-1A , 5-HT1A , 5HT1a , ADRB2RL1 , ADRBRL1 , G-21 , PFMCD

Cellular Localization

Cell membrane

Database

Research Area

DATA IMAGES

Anti-5-HT1A receptor antibody [N3C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104703

GTX104703 ICC/IF Image

5-HT1A receptor antibody [N3C1], Internal detects 5-HT1A receptor protein by immunofluorescent analysis.
Sample: DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: 5-HT1A receptor protein stained by 5-HT1A receptor antibody [N3C1], Internal (GTX104703) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-5-HT1A receptor antibody [N3C1], Internal used in IHC (Frozen sections) (IHC-Fr). GTX104703

GTX104703 IHC-Fr Image

5-HT1A receptor antibody [N3C1], Internal detects 5-HT1A receptor protein by immunohistochemical analysis.
Samples: Frozen Sectioned adult mouse hippocampus.
Green: 5-HT1A receptor protein stained by 5-HT1A receptor antibody [N3C1], Internal (GTX104703) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 10 min

Anti-5-HT1A receptor antibody [N3C1], Internal used in Western Blot (WB). GTX104703

GTX104703 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with 5-HT1A receptor antibody [N3C1], Internal (GTX104703) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-5-HT1A receptor antibody [N3C1], Internal used in Western Blot (WB). GTX104703

GTX104703 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with 5-HT1A receptor antibody [N3C1], Internal (GTX104703) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

2021; : e12771 Melatonin pretreatment alleviates the long-term synaptic toxicity and dysmyelination induced by neonatal Sevoflurane exposure via MT1 receptor-mediated Wnt signaling modulation.
Vegas-Su?rez S et al. Br J Pharmacol 2021; 178 (12) : 2516-2532 The effect of 5-HT1A receptor agonists on the entopeduncular nucleus is modified in 6-hydroxydopamine-lesioned rats.
Chen L et al. J Int Med Res 2020; 48 (5) : 3.00061E+14 Electroacupuncture improves synaptic plasticity by regulating the 5-HT1A receptor in hippocampus of rats with chronic unpredictable mild stress.
Kim J et al. Biomol Ther (Seoul) 2020; 28 (3) : 282-291 Toluene Inhalation Causes Early Anxiety and Delayed Depression with Regulation of Dopamine Turnover, 5-HT1A Receptor, and Adult Neurogenesis in Mice.
Sugimoto K et al. Int J Mol Sci 2020; 22 (1) Serotonin/5-HT1A Signaling in the Neurovascular Unit Regulates Endothelial CLDN5 Expression.
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REVIEW

5-HT1A receptor antibody [N3C1], Internal Cat No. GTX104703

Rating
( Average 5 based on 1 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-5-HT1A receptor antibody [N3C1], Internal used in Western Blot (WB). GTX104703
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Front Behav Neurosci in 2015.PMID: 25870546
Submit a Review
Application Reference
2021;:e12771 Melatonin pretreatment alleviates the long-term synaptic toxicity and dysmyelination induced by neonatal Sevoflurane exposure via MT1 receptor-mediated Wnt signaling modulation.
Vegas-Su?rez S et al. Br J Pharmacol 2021; 178 (12):2516-2532 The effect of 5-HT1A receptor agonists on the entopeduncular nucleus is modified in 6-hydroxydopamine-lesioned rats.
Chen L et al. J Int Med Res 2020; 48 (5):3.00061E+14 Electroacupuncture improves synaptic plasticity by regulating the 5-HT1A receptor in hippocampus of rats with chronic unpredictable mild stress.
Kim J et al. Biomol Ther (Seoul) 2020; 28 (3):282-291 Toluene Inhalation Causes Early Anxiety and Delayed Depression with Regulation of Dopamine Turnover, 5-HT1A Receptor, and Adult Neurogenesis in Mice.
Sugimoto K et al. Int J Mol Sci 2020; 22 (1) Serotonin/5-HT1A Signaling in the Neurovascular Unit Regulates Endothelial CLDN5 Expression.
Vegas-Su?rez S et al. Br J Pharmacol 2020; 6-Hydroxydopamine lesion and levodopa treatment modify the effect of buspirone in the substantia nigra pars reticulata.
Reactivity : Rat
Rudyk C et al. J Neuroinflammation 2019; 16 (1):120 Leucine-rich repeat kinase-2 (LRRK2) modulates paraquat-induced inflammatory sickness and stress phenotype.
Application : WB
Reactivity : Mouse
Lim J et al. J Neurosci 2018; (Epub) LRRK2 G2019S induces anxiety/depression-like behavior prior to the onset of motor dysfunction with 5-HT1A receptor upregulation in mice.
Huang F et al. Front Behav Neurosci 2015; 9:70 Deletion of mouse FXR gene disturbs multiple neurotransmitter systems and alters neurobehavior.
Nakamura Y et al. Biotechnol Bioeng 2015; Applications of yeast-based signaling sensor for characterization of antagonist and analysis of site-directed mutants of the human serotonin 1A receptor.
Application : WB
Reactivity : Human
Lin YL et al. Brain Behav Immun 2012; 26 (3):459-68 Prenatal lipopolysaccharide exposure increases anxiety-like behaviors and enhances stress-induced corticosterone responses in adult rats.
Application : WB
Reactivity : Rat
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169