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5-HT1A receptor antibody [N3C1], Internal

Anti-5-HT1A receptor antibody [N3C1], Internal used in Western Blot (WB). GTX104703
Anti-5-HT1A receptor antibody [N3C1], Internal used in Western Blot (WB). GTX104703
Anti-5-HT1A receptor antibody [N3C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104703
Anti-5-HT1A receptor antibody [N3C1], Internal used in IHC (Frozen sections) (IHC-Fr). GTX104703
Anti-5-HT1A receptor antibody [N3C1], Internal used in Western Blot (WB). GTX104703
Anti-5-HT1A receptor antibody [N3C1], Internal used in Western Blot (WB). GTX104703
Anti-5-HT1A receptor antibody [N3C1], Internal used in Western Blot (WB). GTX104703

Cat. No. GTX104703

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-Fr

Reactivity

Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Predict Reactivity

Rabbit, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.03 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide encompassing a sequence within the Intracellular domain of human 5HT1A Receptor. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1241307

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

5-hydroxytryptamine receptor 1A , 5-HT-1A , 5-HT1A , 5HT1a , ADRB2RL1 , ADRBRL1 , G-21 , PFMCD

Cellular Localization

Cell membrane

Database

Research Area

DATA IMAGES

Anti-5-HT1A receptor antibody [N3C1], Internal used in Western Blot (WB). GTX104703

GTX104703 WB Image

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with 5-HT1A receptor antibody [N3C1], Internal (GTX104703) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-5-HT1A receptor antibody [N3C1], Internal used in Western Blot (WB). GTX104703

GTX104703 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with 5-HT1A receptor antibody [N3C1], Internal (GTX104703) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-5-HT1A receptor antibody [N3C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104703

GTX104703 ICC/IF Image

5-HT1A receptor antibody [N3C1], Internal detects 5-HT1A receptor protein by immunofluorescent analysis.
Sample: DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: 5-HT1A receptor protein stained by 5-HT1A receptor antibody [N3C1], Internal (GTX104703) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-5-HT1A receptor antibody [N3C1], Internal used in IHC (Frozen sections) (IHC-Fr). GTX104703

GTX104703 IHC-Fr Image

5-HT1A receptor antibody [N3C1], Internal detects 5-HT1A receptor protein by immunohistochemical analysis.
Samples: Frozen Sectioned adult mouse hippocampus.
Green: 5-HT1A receptor protein stained by 5-HT1A receptor antibody [N3C1], Internal (GTX104703) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 10 min

Anti-5-HT1A receptor antibody [N3C1], Internal used in Western Blot (WB). GTX104703

GTX104703 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with 5-HT1A receptor antibody [N3C1], Internal (GTX104703) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-5-HT1A receptor antibody [N3C1], Internal used in Western Blot (WB). GTX104703

GTX104703 WB Image

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with 5-HT1A receptor antibody [N3C1], Internal (GTX104703) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-5-HT1A receptor antibody [N3C1], Internal used in Western Blot (WB). GTX104703

GTX104703 WB Image

The data was published in the journal Front Behav Neurosci in 2015.PMID: 25870546

REFERENCE

REVIEW

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Application Reference
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169