GeneTex
United States (US)

5-HT1B receptor antibody [C1C3]

GTX100009_WB_19011505_768.jpg
GTX100009_39224_20170719_IFA_R_19011506_558.jpg

Cat No. GTX100009

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Positive Control

HeLaS3 , HepG2

Predict Reactivity

Mouse, Rabbit, Cat, Dog, Pig, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human 5HT1B Receptor. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

5-Hydroxytryptamine Receptor 1B,5-Ht-1B,5-Ht-1D-Beta,5-Ht1B,5-Ht1Db,Htr1D2,Htr1Db,S12,Htr1B

Cellular Localization

Cell membrane

Background

The neurotransmitter serotonin (5-hydroxytryptamine; 5-HT) exerts a wide variety of physiologic functions through a multiplicity of receptors and may be involved in human neuropsychiatric disorders such as anxiety, depression, or migraine. These receptors consist of 4 main groups, 5-HT-1, 5-HT-2, 5-HT-3, and 5-HT4, subdivided into several distinct subtypes on the basis of their pharmacologic characteristics, coupling to intracellular second messengers, and distribution within the nervous system (Zifa and Fillion, 1992 [PubMed 1359584]). The serotonergic receptors belong to the multigene family of receptors coupled to guanine nucleotide-binding proteins.[supplied by OMIM]

Database

Research Area

DATA IMAGES
GTX100009_WB_19011505_768.jpg

GTX100009 WB Image

Sample(30 μg of whole cell lysate)
A:HeLa S3 (GTX14654)
12% SDS PAGE
GTX100009 diluted at 1:500

GTX100009_39224_20170719_IFA_R_19011506_558.jpg

GTX100009 ICC/IF Image

5-HT1B receptor antibody [C1C3] detects 5-HT1B receptor protein by immunofluorescent analysis.
Sample: DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: 5-HT1B receptor protein stained by 5-HT1B receptor antibody [C1C3] (GTX100009) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Document
Unmodified.pdf
Package List Price ($)
$ 319
$ 169