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5-HT2B receptor antibody

Anti-5-HT2B receptor antibody used in Western Blot (WB). GTX129900
Anti-5-HT2B receptor antibody used in Western Blot (WB). GTX129900

Cat No. GTX129900

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000

Note :

WB
As is commonly seen with membrane proteins, significant hydrophobicity can lead to aggregation following boiling of samples prior to SDS-PAGE and subsequent western blotting. We recommend to avoid boiling in this case.

Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

SH-SY5Y , SH-SY5Y membrane fraction extract , unboild SH-SY5Y , unboiled SH-SY5Y membrane fraction extract , mouse liver

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.29 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human 5-HT2B receptor. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

5-hydroxytryptamine receptor 2B , 5-HT(2B) , 5-HT-2B , 5-HT2B

Cellular Localization

Cell membrane

Background

Multiple receptor subtypes of serotonin neurotransmitters with multiple physiologic functions have been recognized. The 5-HT-2 receptors mediate many of the central and peripheral physiologic functions of serotonin. Cardiovascular effects include contraction of blood vessels and shape changes in platelets; central nervous system effects include neuronal sensitization to tactile stimuli and mediation of hallucinogenic effects of phenylisopropylamine hallucinogens.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-5-HT2B receptor antibody used in Western Blot (WB). GTX129900

GTX129900 WB Image

Boiled and unboiled SH-SY5Y cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with 5-HT2B Receptor antibody (GTX129900) diluted at 1:1000. (WCE: whole cell extract; ME: membrane extract)

Anti-5-HT2B receptor antibody used in Western Blot (WB). GTX129900

GTX129900 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with 5-HT2B Receptor antibody (GTX129900) diluted at 1:1000.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169