GeneTex
United States (US)

5-HT2C receptor antibody

GTX100329_40051_20160310_WB_R_brain_19011506_907.jpg
GTX100329_40051_20160317_WB_19011506_603.jpg
GTX100329_40051_IHC_19011506_934.jpg
GTX100329_40051_20160317_WB_M_placenta_19011506_368.jpg

Cat No. GTX100329

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Positive Control

IMR32 , SK-N-AS , mouse placenta , rat brain

Predict Reactivity

Dog, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.68mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human 5HT2C Receptor. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

5-Hydroxytryptamine Receptor 2C,5-Ht1C,5-Ht2C,5-Htr2C,5Htr2C,Htr1C,Htr2C

Cellular Localization

Cell membrane

Background

Serotonin (5-hydroxytryptamine, 5-HT), a neurotransmitter, elicits a wide array of physiological effects by binding to several receptor subtypes, including the 5-HT2 family of seven-transmembrane-spanning, G-protein-coupled receptors, which activate phospholipase C and D signaling pathways. This gene encodes the 2C subtype of serotonin receptor and its mRNA is subject to multiple RNA editing events, where genomically encoded adenosine residues are converted to inosines. RNA editing is predicted to alter amino acids within the second intracellular loop of the 5-HT2C receptor and generate receptor isoforms that differ in their ability to interact with G proteins and the activation of phospholipase C and D signaling cascades, thus modulating serotonergic neurotransmission in the central nervous system. Studies in humans have reported abnormalities in patterns of 5-HT2C editing in depressed suicide victims. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100329_40051_20160310_WB_R_brain_19011506_907.jpg

GTX100329 WB Image

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with 5-HT2C receptor antibody (GTX100329) diluted at 1:1000.

GTX100329_40051_20160317_WB_19011506_603.jpg

GTX100329 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with 5-HT2C receptor antibody (GTX100329) diluted at 1:500. The signal was developed with Trident ECL plus-Enhanced.

GTX100329_40051_IHC_19011506_934.jpg

GTX100329 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human prostate, using 5HT2C Receptor(GTX100329) antibody(10 μg/ml).
Antigen Retrieval: Citrate buffer, pH 6.0, 10 min

GTX100329_40051_20160317_WB_M_placenta_19011506_368.jpg

GTX100329 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with 5-HT2C receptor antibody (GTX100329) diluted at 1:500. The signal was developed with Trident ECL plus-Enhanced.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169