APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
IMR32 , SK-N-AS , mouse placenta , rat brain
Predict Reactivity
Dog, Chimpanzee(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.68 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of Human 5-HT2C receptor. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
5-hydroxytryptamine receptor 2C , 5-HT1C , 5-HT2C , 5-HTR2C , 5HTR2C , HTR1C
Cellular Localization
Cell membrane
Background
Serotonin (5-hydroxytryptamine, 5-HT), a neurotransmitter, elicits a wide array of physiological effects by binding to several receptor subtypes, including the 5-HT2 family of seven-transmembrane-spanning, G-protein-coupled receptors, which activate phospholipase C and D signaling pathways. This gene encodes the 2C subtype of serotonin receptor and its mRNA is subject to multiple RNA editing events, where genomically encoded adenosine residues are converted to inosines. RNA editing is predicted to alter amino acids within the second intracellular loop of the 5-HT2C receptor and generate receptor isoforms that differ in their ability to interact with G proteins and the activation of phospholipase C and D signaling cascades, thus modulating serotonergic neurotransmission in the central nervous system. Studies in humans have reported abnormalities in patterns of 5-HT2C editing in depressed suicide victims. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX100329 WB Image
Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with 5-HT2C receptor antibody (GTX100329) diluted at 1:1000.
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GTX100329 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with 5-HT2C receptor antibody (GTX100329) diluted at 1:500. The signal was developed with Trident ECL plus-Enhanced.
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GTX100329 IHC-P Image
Immunohistochemical analysis of paraffin-embedded human prostate, using 5HT2C Receptor(GTX100329) antibody(10 μg/ml).
Antigen Retrieval: Citrate buffer, pH 6.0, 10 min
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GTX100329 WB Image
Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with 5-HT2C receptor antibody (GTX100329) diluted at 1:500. The signal was developed with Trident ECL plus-Enhanced.
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REFERENCE
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REVIEW
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