Untreated (–) and treated (+) unboiled mouse tissue extract (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with 5-HT2C receptor antibody (GTX100329) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with 5-HT2C receptor antibody (GTX100329) diluted at 1:1000.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with 5-HT2C receptor antibody (GTX100329) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded human prostate, using 5HT2C Receptor(GTX100329) antibody(10 μg/ml).
Antigen Retrieval: Citrate buffer, pH 6.0, 10 min

Unboiled SH-SY5Y whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with 5-HT2C receptor antibody (GTX100329) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with 5-HT2C receptor antibody (GTX100329) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.