APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.3-1μg/ml |
| Assay dependent |
| 3.75μg/ml |
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89260-PEP).
Product Note
This antibody is expected to recognise all three reported isoforms (NP_998786.2; NP_000860.2; NP_001155244.1).
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-GPQDFEKSPRDR, from the internal region of the protein sequence according to NP_998786.2; NP_000860.2; NP_001155244.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
5-hydroxytryptamine receptor 3A , 5-HT-3 , 5-HT3A , 5-HT3R , 5HT3R , HTR3
Cellular Localization
Cell membrane; Multi-pass membrane protein
Background
The product of this gene belongs to the ligand-gated ion channel receptor superfamily. This gene encodes subunit A of the type 3 receptor for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor causes fast, depolarizing responses in neurons after activation. It appears that the heteromeric combination of A and B subunits is necessary to provide the full functional features of this receptor, since either subunit alone results in receptors with very low conductance and response amplitude. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX89260 WB Image
WB analysis of human colon lysate using GTX89260 5-HT3A receptor antibody, Internal.
Dilution : 0.3μg/ml
Loading : 35μg protein in RIPA buffer
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GTX89260 IHC-P Image
IHC-P analysis of human small intestine using GTX89260 5-HT3A receptor antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75μg/ml
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REFERENCE
There are currently no references for 5-HT3A receptor antibody, Internal (GTX89260). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for 5-HT3A receptor antibody, Internal (GTX89260). Be the first to share your experience with this product.
