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5-Hydroxymethylcytosine / 5-hmC antibody [GT13612]

Anti-5-Hydroxymethylcytosine / 5-hmC antibody [GT13612] used in Methylated DNA Immunoprecipitation (MeDIP). GTX629765
Anti-5-Hydroxymethylcytosine / 5-hmC antibody [GT13612] used in IHC (Paraffin sections) (IHC-P). GTX629765
Anti-5-Hydroxymethylcytosine / 5-hmC antibody [GT13612] used in IHC (Paraffin sections) (IHC-P). GTX629765
Anti-5-Hydroxymethylcytosine / 5-hmC antibody [GT13612] used in Dot blot (Dot). GTX629765
Anti-5-Hydroxymethylcytosine / 5-hmC antibody [GT13612] used in IHC (Paraffin sections) (IHC-P). GTX629765

Cat. No. GTX629765

Reference 9
1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT13612

Isotype

 IgG2b

Application

 IHC-P, IHC-Fr, Dot, EM, MeDIP

Reactivity

 Species independent
Package
100 μl ($399),
25 μl ($169)
See all 5-Hydroxymethylcytosine / 5-hmC products
PRODUCT

Summary

5-hmC antibody recognizes 5-Hydroxymethylcytosine (5-hmC), which is referred to as a “sixth base” of mammalian genomic DNA. 5-hmC is thought to be an epigenetic regulatory modification in its own right, while also being the product of the first TET protein-catalyzed oxidation of 5-methylcytosine in the DNA demethylation pathway.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Dot Assay dependent
EM Assay dependent
MeDIP Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.81 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to 5-HydroxymethylCytosine / 5-hmC

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2736902

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

5hmc Antibody , 5-hmc Antibody , 5-HydroxymethylCytidine Antibody

Research Area

DATA IMAGES

Anti-5-Hydroxymethylcytosine / 5-hmC antibody [GT13612] used in Methylated DNA Immunoprecipitation (MeDIP). GTX629765

GTX629765 MeDIP Image

GTX629765 5-HydroxymethylCytidine antibody [GT13612] in MeDIP experiment.
Human genomic DNA (500 ng) mixed with methylated cytosine standard kit (GTX400004) were subjected to MeDIP with 5-HydroxymethylCytidine antibody [GT13612] (GTX629765) at dilution of 1:500 and analyzed with semi-quantitative PCR. The immunoprecipitated DNA was plotted as % of input DNA.

Anti-5-Hydroxymethylcytosine / 5-hmC antibody [GT13612] used in IHC (Paraffin sections) (IHC-P). GTX629765

GTX629765 IHC-P Image

5-HydroxymethylCytidine antibody [GT13612] detects 5-HydroxymethylCytidine protein at nucleus on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
5-HydroxymethylCytidine antibody [GT13612] (GTX629765) dilution: 1:100.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-5-Hydroxymethylcytosine / 5-hmC antibody [GT13612] used in IHC (Paraffin sections) (IHC-P). GTX629765

GTX629765 IHC-P Image

5-HydroxymethylCytidine antibody [GT13612] detects 5-HydroxymethylCytidine protein at nucleus on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
5-HydroxymethylCytidine antibody [GT13612] (GTX629765) dilution: 1:100.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-5-Hydroxymethylcytosine / 5-hmC antibody [GT13612] used in Dot blot (Dot). GTX629765

GTX629765 Dot Image

GTX629765 5-hydroxymethylcytosine antibody [GT13612] Dot blot analysis of anti-5-hmC antibody with the synthetic DNA controls (GTX400004). DNA samples (0.5 to 50 ng as indicated) were spotted onto the positively charged Nylon membrane and blotted with 5-hmC antibody (GTX629765) at a dilution of 1:500. A: Unmethylated DNA fragment B: DNA fragment containing 5-methylcytosine C: DNA fragment containing 5-hydroxymethylcytosine D: DNA fragment containing 5-formylcytosine E: DNA fragment containing 5-carboxylcytosine

Anti-5-Hydroxymethylcytosine / 5-hmC antibody [GT13612] used in IHC (Paraffin sections) (IHC-P). GTX629765

GTX629765 IHC-P Image

5-HydroxymethylCytidine antibody [GT13612] detects 5-HydroxymethylCytidine protein at nucleus on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
5-HydroxymethylCytidine antibody [GT13612] (GTX629765) dilution: 1:100.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

C Casali et al. Eur J Cell Biol 2023; 103 (1) : 151373 Impact of heat and cold shock on epigenetics and chromatin structure
Application : EM
XX Yan et al. Medicine (Baltimore) 2023; 102 (34) : e34860 The deficiency of 5-methylcytosine (5mC) and its ramification in the occurrence and prognosis of colon cancer
Application : IHC-P
Huang R et al. Ann Transl Med 2020; 8 (6) : 329 Restoration of TET2 deficiency inhibits tumor growth in head neck squamous cell carcinoma.
Wu J et al. J Exp Clin Cancer Res 2019; 38 (1) : 348 TET1-mediated DNA hydroxymethylation activates inhibitors of the Wnt/帣-catenin signaling pathway to suppress EMT in pancreatic tumor cells.
Application : Dot
Yatagai N et al. Virchows Arch 2019; 474 (3) : 315-324 TP53 inactivation and expression of methylation-associated proteins in gastric adenocarcinoma with enteroblastic differentiation.
Application : IHC-P
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REVIEW

5-Hydroxymethylcytosine / 5-hmC antibody [GT13612] Cat. No. GTX629765

Rating
( Average 5 based on 1 users reviews)
Application
Electron microscopy(EM)
( Average 5 based on 1 users reviews)
Anti-5-Hydroxymethylcytosine / 5-hmC antibody [GT13612] used in Electron microscopy (EM). GTX629765
Date : Anonymous submitted on 29-Jun-2018
Score :
Application Tested : EM
Sample : Mouse hepatocyte
Primary Antibody : 1:150, 4°C, 24Hr
Figure Note : Mouse hepatocyte labelled with 5mc (6 nm colloidal gold) and 5hmC (12 nm colloidal gold).
Submit a Review
Application Reference
C Casali et al. Eur J Cell Biol 2023; 103 (1):151373 Impact of heat and cold shock on epigenetics and chromatin structure
Application : EM
XX Yan et al. Medicine (Baltimore) 2023; 102 (34):e34860 The deficiency of 5-methylcytosine (5mC) and its ramification in the occurrence and prognosis of colon cancer
Application : IHC-P
Huang R et al. Ann Transl Med 2020; 8 (6):329 Restoration of TET2 deficiency inhibits tumor growth in head neck squamous cell carcinoma.
Wu J et al. J Exp Clin Cancer Res 2019; 38 (1):348 TET1-mediated DNA hydroxymethylation activates inhibitors of the Wnt/帣-catenin signaling pathway to suppress EMT in pancreatic tumor cells.
Application : Dot
Yatagai N et al. Virchows Arch 2019; 474 (3):315-324 TP53 inactivation and expression of methylation-associated proteins in gastric adenocarcinoma with enteroblastic differentiation.
Application : IHC-P
Sun J et al. Cell Stem Cell 2018; 23 (3):355-369.e9 SIRT1 Activation Disrupts Maintenance of Myelodysplastic Syndrome Stem and Progenitor Cells by Restoring TET2 Function.
Application : Dot
Cesar S.Huertas et al. Biosensors and Bioelectronics 2018; 120:47-54 Label-free DNA-methylation detection by direct ds-DNA fragment screening using poly-purine hairpins.
Hsieh MC et al. J Pineal Res 2017; (Epub) Melatonin impedes Tet1-dependent mGluR5 promoter demethylation to relieve pain.
Application : Dot
Hsieh MC et al. Sci Rep 2016; 6:37411 Tet1-dependent epigenetic modification of BDNF expression in dorsal horn neurons mediates neuropathic pain in rats.
Application : IHC-Fr, Dot
SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations