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5-Hydroxymethylcytosine / 5-hmC antibody [GT13612]

Anti-5-Hydroxymethylcytosine / 5-hmC antibody [GT13612] used in Methylated DNA Immunoprecipitation (MeDIP). GTX629765
Anti-5-Hydroxymethylcytosine / 5-hmC antibody [GT13612] used in IHC (Paraffin sections) (IHC-P). GTX629765
Anti-5-Hydroxymethylcytosine / 5-hmC antibody [GT13612] used in IHC (Paraffin sections) (IHC-P). GTX629765
Anti-5-Hydroxymethylcytosine / 5-hmC antibody [GT13612] used in Dot blot (Dot). GTX629765
Anti-5-Hydroxymethylcytosine / 5-hmC antibody [GT13612] used in IHC (Paraffin sections) (IHC-P). GTX629765

Cat No. GTX629765

Host

Mouse

Clonality

Monoclonal

Clone Name

GT13612

Isotype

IgG2b

Application

IHC-P, IHC-Fr, Dot, EM, MeDIP

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)
PRODUCT

Summary

5-hmC antibody recognizes 5-Hydroxymethylcytosine (5-hmC), which is referred to as a “sixth base” of mammalian genomic DNA. 5-hmC is thought to be an epigenetic regulatory modification in its own right, while also being the product of the first TET protein-catalyzed oxidation of 5-methylcytosine in the DNA demethylation pathway.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Dot Assay dependent
EM Assay dependent
MeDIP Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.81 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to 5-HydroxymethylCytosine / 5-hmC

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

5hmc Antibody , 5-hmc Antibody , 5-HydroxymethylCytidine Antibody

Research Area

DATA IMAGES

Anti-5-Hydroxymethylcytosine / 5-hmC antibody [GT13612] used in Methylated DNA Immunoprecipitation (MeDIP). GTX629765

GTX629765 MeDIP Image

GTX629765 5-HydroxymethylCytidine antibody [GT13612] in MeDIP experiment.
Human genomic DNA (500 ng) mixed with methylated cytosine standard kit (GTX400004) were subjected to MeDIP with 5-HydroxymethylCytidine antibody [GT13612] (GTX629765) at dilution of 1:500 and analyzed with semi-quantitative PCR. The immunoprecipitated DNA was plotted as % of input DNA.

Anti-5-Hydroxymethylcytosine / 5-hmC antibody [GT13612] used in IHC (Paraffin sections) (IHC-P). GTX629765

GTX629765 IHC-P Image

5-HydroxymethylCytidine antibody [GT13612] detects 5-HydroxymethylCytidine protein at nucleus on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
5-HydroxymethylCytidine antibody [GT13612] (GTX629765) dilution: 1:100.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-5-Hydroxymethylcytosine / 5-hmC antibody [GT13612] used in IHC (Paraffin sections) (IHC-P). GTX629765

GTX629765 IHC-P Image

5-HydroxymethylCytidine antibody [GT13612] detects 5-HydroxymethylCytidine protein at nucleus on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
5-HydroxymethylCytidine antibody [GT13612] (GTX629765) dilution: 1:100.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-5-Hydroxymethylcytosine / 5-hmC antibody [GT13612] used in Dot blot (Dot). GTX629765

GTX629765 Dot Image

GTX629765 5-hydroxymethylcytosine antibody [GT13612] Dot blot analysis of anti-5-hmC antibody with the synthetic DNA controls (GTX400004). DNA samples (0.5 to 50 ng as indicated) were spotted onto the positively charged Nylon membrane and blotted with 5-hmC antibody (GTX629765) at a dilution of 1:500. A: Unmethylated DNA fragment B: DNA fragment containing 5-methylcytosine C: DNA fragment containing 5-hydroxymethylcytosine D: DNA fragment containing 5-formylcytosine E: DNA fragment containing 5-carboxylcytosine

Anti-5-Hydroxymethylcytosine / 5-hmC antibody [GT13612] used in IHC (Paraffin sections) (IHC-P). GTX629765

GTX629765 IHC-P Image

5-HydroxymethylCytidine antibody [GT13612] detects 5-HydroxymethylCytidine protein at nucleus on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
5-HydroxymethylCytidine antibody [GT13612] (GTX629765) dilution: 1:100.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

REVIEW

5-Hydroxymethylcytosine / 5-hmC antibody [GT13612] Cat No. GTX629765

Rating ( Average 5 based on 1 users reviews)
Application
Electron microscopy(EM) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 29-Jun-2018
Score :
Application Tested : EM
Sample : Mouse hepatocyte
Primary Antibody : 1:150, 4°C, 24Hr
Figure Note : Mouse hepatocyte labelled with 5mc (6 nm colloidal gold) and 5hmC (12 nm colloidal gold).
SDS
PBS.pdf
Package List Price ($)
$ 329
$ 169