5-Hydroxymethylcytosine / 5-hmC antibody [GT13612]

Name: 5-Hydroxymethylcytosine / 5-hmC antibody [GT13612]
Brand: GeneTex
SKU: GTX629765
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-5-Hydroxymethylcytosine / 5-hmC antibody [GT13612] used in Methylated DNA Immunoprecipitation (MeDIP). GTX629765

Anti-5-Hydroxymethylcytosine / 5-hmC antibody [GT13612] used in Dot blot (Dot). GTX629765

Anti-5-Hydroxymethylcytosine / 5-hmC antibody [GT13612] used in IHC (Paraffin sections) (IHC-P). GTX629765

Cat. No. GTX629765

Reference 7

Host	Mouse
Clonality	Monoclonal
Clone Name	GT13612
Isotype	IgG2b
Application	IHC-P, IHC-Fr, Dot, EM, MeDIP
Reactivity	Species independent
Package	100 μl ($399), 25 μl ($169)

See all 5-Hydroxymethylcytosine / 5-hmC products

PRODUCT

Summary

5-hmC antibody recognizes 5-Hydroxymethylcytosine (5-hmC), which is referred to as a “sixth base” of mammalian genomic DNA. 5-hmC is thought to be an epigenetic regulatory modification in its own right, while also being the product of the first TET protein-catalyzed oxidation of 5-methylcytosine in the DNA demethylation pathway.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
IHC-P	1:100-1:1000
IHC-Fr	Assay dependent
Dot	Assay dependent
EM	Assay dependent
MeDIP	Assay dependent

Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.81 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to 5-HydroxymethylCytosine / 5-hmC

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2736902

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

5hmc Antibody , 5-hmc Antibody , 5-HydroxymethylCytidine Antibody

Research Area

DATA IMAGES

GTX629765 MeDIP Image

GTX629765 5-HydroxymethylCytidine antibody [GT13612] in MeDIP experiment.
Human genomic DNA (500 ng) mixed with methylated cytosine standard kit (GTX400004) were subjected to MeDIP with 5-HydroxymethylCytidine antibody [GT13612] (GTX629765) at dilution of 1:500 and analyzed with semi-quantitative PCR. The immunoprecipitated DNA was plotted as % of input DNA.

GTX629765 Dot Image

GTX629765 5-hydroxymethylcytosine antibody [GT13612] Dot blot analysis of anti-5-hmC antibody with the synthetic DNA controls (GTX400004). DNA samples (0.5 to 50 ng as indicated) were spotted onto the positively charged Nylon membrane and blotted with 5-hmC antibody (GTX629765) at a dilution of 1:500. A: Unmethylated DNA fragment B: DNA fragment containing 5-methylcytosine C: DNA fragment containing 5-hydroxymethylcytosine D: DNA fragment containing 5-formylcytosine E: DNA fragment containing 5-carboxylcytosine

GTX629765 IHC-P Image

5-HydroxymethylCytidine antibody [GT13612] detects 5-HydroxymethylCytidine protein at nucleus on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
5-HydroxymethylCytidine antibody [GT13612] (GTX629765) dilution: 1:100.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX629765 IHC-P Image

5-HydroxymethylCytidine antibody [GT13612] detects 5-HydroxymethylCytidine protein at nucleus on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
5-HydroxymethylCytidine antibody [GT13612] (GTX629765) dilution: 1:100.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min