5-Hydroxymethylcytosine / 5-hmC antibody [GT13612]

Cat. No. GTX629765

GTX629765 MeDIP Image

GTX629765 5-Hydroxymethylcytosine / 5-hmC antibody [GT13612] in MeDIP experiment.
Human genomic DNA (500 ng) mixed with methylated cytosine standard kit (GTX400004) were subjected to MeDIP with 5-Hydroxymethylcytosine / 5-hmC antibody [GT13612] (GTX629765) at dilution of 1:500 and analyzed with semi-quantitative PCR. The immunoprecipitated DNA was plotted as % of input DNA.

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GTX629765 IHC-P Image

5-Hydroxymethylcytosine / 5-hmC antibody [GT13612] detects 5-HydroxymethylCytidine protein at nucleus on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
5-Hydroxymethylcytosine / 5-hmC antibody [GT13612] (GTX629765) dilution: 1:100.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

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GTX629765 Dot Image

GTX629765 5-hydroxymethylcytosine antibody [GT13612] Dot blot analysis of anti-5-hmC antibody with the synthetic DNA controls (GTX400004). DNA samples (0.5 to 50 ng as indicated) were spotted onto the positively charged Nylon membrane and blotted with 5-hmC antibody (GTX629765) at a dilution of 1:500.
A: Unmethylated DNA fragment
B: DNA fragment containing 5-methylcytosine
C: DNA fragment containing 5-hydroxymethylcytosine
D: DNA fragment containing 5-formylcytosine
E: DNA fragment containing 5-carboxylcytosine

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GTX629765 IHC-P Image

5-Hydroxymethylcytosine / 5-hmC antibody [GT13612] detects 5-HydroxymethylCytidine protein at nucleus on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
5-Hydroxymethylcytosine / 5-hmC antibody [GT13612] (GTX629765) dilution: 1:100.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

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GTX629765 IHC-P Image

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This 5-Hydroxymethylcytosine / 5-hmC antibody [GT13612] (GTX629765) is a mouse monoclonal antibody validated for detection of 5-Hydroxymethylcytosine / 5-hmC by ICC/IF, IHC-P, Dot, EM, and MeDIP. Reactivity is species-independent. This product is cited in 11 published studies.

Host

Mouse
Clonality

Monoclonal
Clone Name

GT13612
Isotype

IgG2b
Applications

ICC/IF IHC-P Dot EM MeDIP
Reactivity

Species independent

Summary

5-hmC antibody recognizes 5-Hydroxymethylcytosine (5-hmC), which is referred to as a “sixth base” of mammalian genomic DNA. 5-hmC is thought to be an epigenetic regulatory modification in its own right, while also being the product of the first TET protein-catalyzed oxidation of 5-methylcytosine in the DNA demethylation pathway.