5-Hydroxymethylcytosine / 5-hmC antibody [GT513]

GTX629701 5-Hydroxymethylcytosine/ 5-hmC antibody [GT513] in MeDIP experiment.
Human genomic DNA (500 ng) mixed with methylated cytosine standard kit (GTX400004) were subjected to MeDIP with 5-Hydroxymethylcytosine/ 5-hmC antibody [GT513] (GTX629701) at dilution of 1:500 and analyzed with semi-quantitative PCR. The immunoprecipitated DNA was plotted as % of input DNA.

Dotblot analysis of anti- 5-Hydroxymethylcytosine / 5-hmC antibody [GT513] with DNA samples
DNA samples (50 to 500 ng as indicated) were spotted onto positively charged nylon membrane and blotted with 5-Hydroxymethylcytosine / 5-hmC antibody [GT513] (GTX629701) at a dilution of 1:500.
A: Genomic DNA derived from 293T cells.
B: Genomic DNA derived from human TET-1 transfected 293T cells.

GTX629701 5-hydroxymethylcytosine antibody [GT513] Dot blot analysis of anti-5-hmC antibody with the synthetic DNA controls (GTX400004). DNA samples (0.5 to 50 ng as indicated) were spotted onto the positively charged Nylon membrane and blotted with 5-hmC antibody (GTX629701) at a dilution of 1:500. A: Unmethylated DNA fragment B: DNA fragment containing 5-methylcytosine C: DNA fragment containing 5-hydroxymethylcytosine D: DNA fragment containing 5-formylcytosine E: DNA fragment containing 5-carboxylcytosine