Dot blot analysis of 100 to 4 ng (equivalent of 5 to 0.2 pmol of C-bases) of the hmC, mC and C PCR controls using GTX60802 5-Hydroxymethylcytosine / 5-hmC antibody - MeDIP grade.
Dilution : 1:200

hMeDIP analysis of HeLa cells DNA using GTX60802 5-Hydroxymethylcytosine / 5-hmC antibody - MeDIP grade. 1 μg of human Hela cells DNA were spiked with non-methylated, methylated, and hydroxymethylated fragments. The DNA was prepared with the GenDNA module of the hMeDIP kit and sonicated to have DNA fragments of 300-500 bp. The IgG isotype antibodies from rabbit was used as negative control. The obtained results show that this antibody is highly specific for this base modification (no IP with non-methylated or methylated C bases containing fragments).

ELISA analysis of peptides used for immunization using GTX60802 5-Hydroxymethylcytosine / 5-hmC antibody - MeDIP grade.