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5-Hydroxymethylcytosine / 5-hmC antibody - MeDIP grade

Anti-5-Hydroxymethylcytosine / 5-hmC antibody - MeDIP grade used in ELISA (ELISA). GTX60802
Anti-5-Hydroxymethylcytosine / 5-hmC antibody - MeDIP grade used in Hydroxymethylated DNA immunoprecipitation (hMeDIP). GTX60802
Anti-5-Hydroxymethylcytosine / 5-hmC antibody - MeDIP grade used in Dot blot (Dot). GTX60802

Cat. No. GTX60802

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 Dot, ELISA, hMeDIP

Reactivity

 Species independent
Package
50 μl ($399)
See all 5-Hydroxymethylcytosine / 5-hmC products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
Dot 1:200
ELISA 1/500
hMeDIP 2.5 μl
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

Serum

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Immunogen

5-hydroxymethylcytosine conjugated to KLH.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-5-Hydroxymethylcytosine / 5-hmC antibody - MeDIP grade used in ELISA (ELISA). GTX60802

GTX60802 ELISA Image

ELISA analysis of peptides used for immunization using GTX60802 5-Hydroxymethylcytosine / 5-hmC antibody - MeDIP grade.

Anti-5-Hydroxymethylcytosine / 5-hmC antibody - MeDIP grade used in Hydroxymethylated DNA immunoprecipitation (hMeDIP). GTX60802

GTX60802 hMeDIP Image

hMeDIP analysis of HeLa cells DNA using GTX60802 5-Hydroxymethylcytosine / 5-hmC antibody - MeDIP grade. 1 μg of human Hela cells DNA were spiked with non-methylated, methylated, and hydroxymethylated fragments. The DNA was prepared with the GenDNA module of the hMeDIP kit and sonicated to have DNA fragments of 300-500 bp. The IgG isotype antibodies from rabbit was used as negative control. The obtained results show that this antibody is highly specific for this base modification (no IP with non-methylated or methylated C bases containing fragments).

Anti-5-Hydroxymethylcytosine / 5-hmC antibody - MeDIP grade used in Dot blot (Dot). GTX60802

GTX60802 Dot Image

Dot blot analysis of 100 to 4 ng (equivalent of 5 to 0.2 pmol of C-bases) of the hmC, mC and C PCR controls using GTX60802 5-Hydroxymethylcytosine / 5-hmC antibody - MeDIP grade.
Dilution : 1:200

REFERENCE

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REVIEW

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Package List Price ($)
$ 399