GeneTex
United States (US)

5-Hydroxymethylcytosine / 5-hmC antibody [RM236]

GTX33605_20160517_ELISA_18121410_262.jpg
GTX33605_20160517_Dot_18121410_547.jpg
GTX33605_20160517_IHC-P_18121410_731.jpg

Cat No. GTX33605

Host Rabbit
Clonality Monoclonal
Clone Name RM236
Isotype IgG
Application ICC/IF, IHC-P, Dot, ELISA, MeDIP
APPLICATION

Application Note

Dot Blot: 0.2 ug/mL – 1 ug/mL, ELISA: 0.1 ug/mL - 1 ug/mL, IHC: 0.1 ug/mL - 1 ug/mL.

Specificity/Sensitivity

This antibody reacts to 5-hydroxymethylcytosine in both single-stranded and double-stranded DNA. No cross reactivity with non-methylated cytosine and methylcytosine in DNA.
PROPERTIES

Form

Liquid

Buffer

50% Glycerol/PBS with 1% BSA and 0.09% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

BSA-conjugated 5-hydroxymethylcytosine.

Purification

Protein A affinity purified from an animal origin-free culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
DATA IMAGES
GTX33605_20160517_ELISA_18121410_262.jpg

GTX33605 ELISA Image

ELISA of single stranded DNA using rabbit monoclonal anti-5-hmC (clone RM236) antibody. The plate was coatedwith streptavidin and then biotinylated single stranded unmethylated DNA, 5-Methylcytosine (5-mC) DNA, and 5-Hydroxymethylcytosine (5-hmC) DNA. A serial dilution of RM236 was used as the primary antibody, and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

GTX33605_20160517_Dot_18121410_547.jpg

GTX33605 Dot Image

Dot blot of double stranded DNA using rabbit monoclonal anti-5-hmC (clone RM236) antibody. The membrane was pre-spotted with 50, 5, and 0.5 ng/dot of double stranded 5-Hydroxymethylcytosine (5-hmC) DNA, 5-Methylcytosine (5-mC) DNA, and unmethylated DNA. The pre-spotted membrane was then blotted with RM236.

GTX33605_20160517_IHC-P_18121410_731.jpg

GTX33605 IHC-P Image

Immunohistochemical staining of formalin fixed and paraffin embedded human brain tissue sections, using rabbit monoclonal anti-5-hmC (clone RM236) antibody.

Package List Price ($)
$ 349