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5-Hydroxymethylcytosine / 5-hmC antibody [RM236]

Anti-5-Hydroxymethylcytosine / 5-hmC antibody [RM236] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33605
Anti-5-Hydroxymethylcytosine / 5-hmC antibody [RM236] used in ELISA (ELISA). GTX33605
Anti-5-Hydroxymethylcytosine / 5-hmC antibody [RM236] used in ELISA (ELISA). GTX33605
Anti-5-Hydroxymethylcytosine / 5-hmC antibody [RM236] used in Methylated DNA Immunoprecipitation (MeDIP). GTX33605
Anti-5-Hydroxymethylcytosine / 5-hmC antibody [RM236] used in IHC (Paraffin sections) (IHC-P). GTX33605
Anti-5-Hydroxymethylcytosine / 5-hmC antibody [RM236] used in Dot blot (Dot). GTX33605
Anti-5-Hydroxymethylcytosine / 5-hmC antibody [RM236] used in Flow cytometry (FACS). GTX33605

Cat. No. GTX33605

Reference 1

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 RM236

Isotype

 IgG

Application

 ICC/IF, IHC-P, FACS, Dot, ELISA, MeDIP

Reactivity

 Species independent
Package
50 μg ($479)
See all 5-Hydroxymethylcytosine / 5-hmC products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF 0.5 μg/mL - 2 μg/mL
IHC-P 0.1 μg/mL - 1 μg/mL
FACS 1 μg/mL - 5 μg/mL
Dot 0.2 μg/mL - 1 μg/mL
ELISA 0.1 μg/mL - 1 μg/mL
MeDIP 0.2 μg/mL - 2 μg/mL
Not tested in other applications.

Product Note

This antibody reacts to 5-hydroxymethylcytosine in both single-stranded and double-stranded DNA. No cross reactivity with non-methylated cytosine and methylcytosine in DNA.

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Immunogen

BSA-conjugated 5-hydroxymethylcytosine.

Purification

Protein A purified
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_2887724

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-5-Hydroxymethylcytosine / 5-hmC antibody [RM236] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33605

GTX33605 ICC/IF Image

ICC/IF analysis of HeLa cells treated with sodium butyrate using GTX33605 5-Hydroxymethylcytosine / 5-hmC antibody [RM236]. HeLa cells were fixed with 4% PFA and permeabilized with methanol (-20ºC) before treatment with 2 N HCl for 30 min at 37 ºC to denature DNA.
Red : Primary antibody
Green : Actin
Dilution : 0.5μg/ml

Anti-5-Hydroxymethylcytosine / 5-hmC antibody [RM236] used in ELISA (ELISA). GTX33605

GTX33605 ELISA Image

ELISA analysis of mouse brain genomic DNA using GTX33605 5-Hydroxymethylcytosine / 5-hmC antibody [RM236].
Dilution : 1 ug/mL or 3 ug/mL of RM236 was used as the primary antibody.

Anti-5-Hydroxymethylcytosine / 5-hmC antibody [RM236] used in ELISA (ELISA). GTX33605

GTX33605 ELISA Image

ELISA analysis of unmethylated DNA, 5-Methylcytosine (5-mC) DNA, and 5-Hydroxymethylcytosine (5-hmC) DNA using serial diluted GTX33605 5-Hydroxymethylcytosine / 5-hmC antibody [RM236].

Anti-5-Hydroxymethylcytosine / 5-hmC antibody [RM236] used in Methylated DNA Immunoprecipitation (MeDIP). GTX33605

GTX33605 MeDIP Image

MeDIP analysis of 1 ng of unmethylated, 5-Methylcytosine (5-mC) or 5-Hydroxymethylcytosine (5-hmC) DNA standard (897 bp) spiked in 1ug of genomic DNA isolated from HeLa cells using GTX33605 5-Hydroxymethylcytosine / 5-hmC antibody [RM236]. MeDIP was performed at a 10:1 DNA:Ab ratio. Realtime PCR was then performed to determine the capture of DNA standard as in % of input.

Anti-5-Hydroxymethylcytosine / 5-hmC antibody [RM236] used in IHC (Paraffin sections) (IHC-P). GTX33605

GTX33605 IHC-P Image

IHC-P analysis of human brain tissue using GTX33605 5-Hydroxymethylcytosine / 5-hmC antibody [RM236].

Anti-5-Hydroxymethylcytosine / 5-hmC antibody [RM236] used in Dot blot (Dot). GTX33605

GTX33605 Dot Image

Dot blot analysis of 50, 5, and 0.5 ng/dot of double stranded 5-Hydroxymethylcytosine (5-hmC) DNA, 5-Methylcytosine (5-mC) DNA, and unmethylated DNA using GTX33605 5-Hydroxymethylcytosine / 5-hmC antibody [RM236].

Anti-5-Hydroxymethylcytosine / 5-hmC antibody [RM236] used in Flow cytometry (FACS). GTX33605

GTX33605 FACS Image

FACS analysis of HEK293 cells using GTX33605 5-Hydroxymethylcytosine / 5-hmC antibody [RM236]. The cells were fixed with ice-cold MeOH, permeabilized with 0.5% Triton X-100, denatured with 2N HCl, then stained with GTX33605 (Blue) or with a negative control PEG antibody (Red).

REFERENCE

Application Reference

Kojima N et al. Biosens Bioelectron 2020; 167 : 112472 Quantitative analysis of global 5-methyl- and 5-hydroxymethylcytosine in TET1 expressed HEK293T cells.
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REVIEW

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Package List Price ($)
$ 479