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5-Hydroxymethylcytosine / 5-hmC antibody

Anti-5-Hydroxymethylcytosine / 5-hmC antibody used in Methylated DNA Immunoprecipitation (MeDIP). GTX128454
Anti-5-Hydroxymethylcytosine / 5-hmC antibody used in Dot blot (Dot). GTX128454

Cat. No. GTX128454

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 Dot, MeDIP

Reactivity

 Species independent
Package
100 μl ($399),
25 μl ($169)
See all 5-Hydroxymethylcytosine / 5-hmC products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
Dot Assay dependent
MeDIP Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

7.65 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to 5-HydroxymethylCytosine / 5-hmC

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885779

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-5-Hydroxymethylcytosine / 5-hmC antibody used in Methylated DNA Immunoprecipitation (MeDIP). GTX128454

GTX128454 MeDIP Image

GTX128454 5-Hydroxymethylcytosine/ 5-hmC antibody in MeDIP experiment.
Human genomic DNA (150 ng) mixed with methylated cytosine standard kit (GTX400004) were subjected to MeDIP with 5-Hydroxymethylcytosine/ 5-hmC antibody (GTX128454) at dilution of 1:2000 and analyzed with semi-quantitative PCR. The immunoprecipitated DNA was plotted as % of input DNA.

Anti-5-Hydroxymethylcytosine / 5-hmC antibody used in Dot blot (Dot). GTX128454

GTX128454 Dot Image

GTX128454 5-hydroxymethylcytosine antibody Dot blot analysis of anti-5-hmC antibody with the synthetic DNA controls (GTX400004). DNA samples (0.5 to 50 ng as indicated) were spotted onto the positively charged Nylon membrane and blotted with 5-hmC antibody (GTX128454) at a dilution of 1:50000.
A: Unmethylated DNA
B: DNA containing 5-methylcytosine
C: DNA containing 5-hydroxymethylcytosine
D: DNA containing 5-formylcytosine
E: DNA containing 5-carboxylcytosine

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169