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5-Hydroxymethylcytosine / 5-hmC antibody

Anti-5-Hydroxymethylcytosine / 5-hmC antibody used in Methylated DNA Immunoprecipitation (MeDIP). GTX128454
Anti-5-Hydroxymethylcytosine / 5-hmC antibody used in Dot blot (Dot). GTX128454

Cat No. GTX128454

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

Dot, MeDIP
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
Dot Assay dependent
MeDIP Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

7.55 mg/ml (Please refer to the vial label for the specific concentration.)

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

DATA IMAGES

Anti-5-Hydroxymethylcytosine / 5-hmC antibody used in Methylated DNA Immunoprecipitation (MeDIP). GTX128454

GTX128454 MeDIP Image

GTX128454 5-Hydroxymethylcytosine/ 5-hmC antibody in MeDIP experiment.
Human genomic DNA (150 ng) mixed with methylated cytosine standard kit (GTX400004) were subjected to MeDIP with 5-Hydroxymethylcytosine/ 5-hmC antibody (GTX128454) at dilution of 1:2000 and analyzed with semi-quantitative PCR. The immunoprecipitated DNA was plotted as % of input DNA.

Anti-5-Hydroxymethylcytosine / 5-hmC antibody used in Dot blot (Dot). GTX128454

GTX128454 Dot Image

GTX128454 5-hydroxymethylcytosine antibody Dot blot analysis of anti-5-hmC antibody with the synthetic DNA controls (GTX400004). DNA samples (0.5 to 50 ng as indicated) were spotted onto the positively charged Nylon membrane and blotted with 5-hmC antibody (GTX128454) at a dilution of 1:50000.
A: Unmethylated DNA
B: DNA containing 5-methylcytosine
C: DNA containing 5-hydroxymethylcytosine
D: DNA containing 5-formylcytosine
E: DNA containing 5-carboxylcytosine

REFERENCE

There are currently no references for 5-Hydroxymethylcytosine / 5-hmC antibody (GTX128454). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for 5-Hydroxymethylcytosine / 5-hmC antibody (GTX128454). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169