5-Lipoxygenase antibody [GT1241]

Cat. No. GTX02838

Host	Rabbit
Clonality	Monoclonal
Clone Name	GT1241
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse, Rat
Package	100 μl ($419)

See all 5-Lipoxygenase products

Datasheet

View product citations for antibody GTX02838 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500 - 1:2000
ICC/IF	1:50 - 1:200
IHC-P	1:50 - 1:200

Not tested in other applications.

Calculated MW

78 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, we recommend adding 50% glycerol, then aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.45 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human ALOX5

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

arachidonate 5-lipoxygenase , 5-LO , 5-LOX , 5LPG , LOG5

Cellular Localization

Cytoplasm

Background

This gene encodes a member of the lipoxygenase gene family and plays a dual role in the synthesis of leukotrienes from arachidonic acid. The encoded protein, which is expressed specifically in bone marrow-derived cells, catalyzes the conversion of arachidonic acid to 5(S)-hydroperoxy-6-trans-8,11,14-cis-eicosatetraenoic acid, and further to the allylic epoxide 5(S)-trans-7,9-trans-11,14-cis-eicosatetrenoic acid (leukotriene A4). Leukotrienes are important mediators of a number of inflammatory and allergic conditions. Mutations in the promoter region of this gene lead to a diminished response to antileukotriene drugs used in the treatment of asthma and may also be associated with atherosclerosis and several cancers. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]

Database

Gene ID: 240 ALOX5
UniProt: P09917 ALOX5

Research Area

DATA IMAGES

GTX02838 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with 5-Lipoxygenase antibody [GT1241] (GTX02838) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX02838 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with 5-Lipoxygenase antibody [GT1241] (GTX02838) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX02838 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX02838 5-Lipoxygenase antibody [GT1241].
Dilution : 1:100
Blue : DAPI for nuclear staining.

GTX02838 IHC-P Image

IHC-P analysis of mouse spleen tissue section using GTX02838 5-Lipoxygenase antibody [GT1241].
Dilution : 1:100

GTX02838 WB Image

WB analysis of rat lung tissue lysates using GTX02838 5-Lipoxygenase antibody [GT1241].
Dilution : 1:1000
Loading : 25μg

GTX02838 WB Image

WB analysis of Raji whole cell lysate using GTX02838 5-Lipoxygenase antibody [GT1241].
Dilution : 1:1000
Loading : 25μg

GTX02838 IHC-P Image

IHC-P analysis of human appendix tissue section using GTX02838 5-Lipoxygenase antibody [GT1241].
Dilution : 1:100

REFERENCE

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REVIEW

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Package

List Price ($)

100 μl

$ 419

Qty

5-Lipoxygenase antibody [GT1241]

Cat. No. GTX02838

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX02838 WB Image

GTX02838 WB Image

GTX02838 ICC/IF Image

GTX02838 IHC-P Image

GTX02838 WB Image

GTX02838 WB Image

GTX02838 IHC-P Image

REFERENCE

REVIEW

Product

Research

Support

Company

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All products are for research use only—Not for use in diagnostic or therapeutic applications.