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5-Methylcytosine / 5-mC antibody [GT4111]

Anti-5-Methylcytosine / 5-mC antibody [GT4111] used in Methylated DNA Immunoprecipitation (MeDIP). GTX629448
Anti-5-Methylcytosine / 5-mC antibody [GT4111] used in IHC (Whole mount) (IHC-Wm). GTX629448
Anti-5-Methylcytosine / 5-mC antibody [GT4111] used in IHC (Whole mount) (IHC-Wm). GTX629448
Anti-5-Methylcytosine / 5-mC antibody [GT4111] used in Dot blot (Dot). GTX629448
Anti-5-Methylcytosine / 5-mC antibody [GT4111] used in IHC (Paraffin sections) (IHC-P). GTX629448
Anti-5-Methylcytosine / 5-mC antibody [GT4111] used in IHC (Paraffin sections) (IHC-P). GTX629448
Anti-5-Methylcytosine / 5-mC antibody [GT4111] used in IHC (Paraffin sections) (IHC-P). GTX629448
Anti-5-Methylcytosine / 5-mC antibody [GT4111] used in IHC (Paraffin sections) (IHC-P). GTX629448

Cat No. GTX629448

Host Mouse
Clonality Monoclonal
Clone Name GT4111
Isotype IgG1
Application IHC-P, IHC-Fr, IHC-Wm, Dot, EM, MeDIP
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
IHC-Wm 1:50-1:500
Dot Assay dependent
EM Assay dependent
MeDIP Assay dependent
Not tested in other applications.

Positive Control

GTX400004 , *SH-SY5Y genomic DNA ,
PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.7 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to 5-Methylcytosine / 5-mC

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
DATA IMAGES
Anti-5-Methylcytosine / 5-mC antibody [GT4111] used in Methylated DNA Immunoprecipitation (MeDIP). GTX629448

GTX629448 MeDIP Image

GTX629448 5-Methylcytosine/ 5-mC antibody [GT4111] in MeDIP experiment.
Human genomic DNA (500 ng) mixed with methylated cytosine standard kit (GTX400004) were subjected to MeDIP with 5-Methylcytosine/ 5-mC antibody [GT4111] (GTX629448) at dilution of 1:500 and analyzed with semi-quantitative PCR. The immunoprecipitated DNA was plotted as % of input DNA.

Anti-5-Methylcytosine / 5-mC antibody [GT4111] used in IHC (Whole mount) (IHC-Wm). GTX629448

GTX629448 IHC-Wm Image

5-Methylcytosine / 5-mC antibody [GT4111] detects 5-Methylcytosine / 5-mC protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 24 hours-post-fertilization zebrafish embryo.
5-Methylcytosine / 5-mC antibody [GT4111] (GTX629448) dilution: 1:50.

Anti-5-Methylcytosine / 5-mC antibody [GT4111] used in IHC (Whole mount) (IHC-Wm). GTX629448

GTX629448 IHC-Wm Image

5-Methylcytosine / 5-mC antibody [GT4111] detects 5-Methylcytosine / 5-mC protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 24 hours-post-fertilization zebrafish embryo.
5-Methylcytosine / 5-mC antibody [GT4111] (GTX629448) dilution: 1:50.

Anti-5-Methylcytosine / 5-mC antibody [GT4111] used in Dot blot (Dot). GTX629448

GTX629448 Dot Image

GTX629448 5-methylcytosine antibody [GT4111] Dot blot analysis of anti-5-mC antibody with the synthetic DNA controls (GTX400004). DNA samples (0.5 to 50 ng) were spotted onto positively charged nylon membrane and blotted with 5-mC antibody (GTX629448) at a dilution of 1:500. A: Unmethylated DNA fragment B: DNA fragment containing 5-methylcytosine C: DNA fragment containing 5-hydroxymethylcytosine D: DNA fragment containing 5-formylcytosine E: DNA fragment containing 5-carboxylcytosine

Anti-5-Methylcytosine / 5-mC antibody [GT4111] used in IHC (Paraffin sections) (IHC-P). GTX629448

GTX629448 IHC-P Image

5-Methylcytosine / 5-mC antibody [GT4111] detects 5-Methylcytosine / 5-mC protein at nucleus on human ovarian carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian carcinoma.
5-Methylcytosine / 5-mC antibody [GT4111] (GTX629448) dilution: 1:200.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-5-Methylcytosine / 5-mC antibody [GT4111] used in IHC (Paraffin sections) (IHC-P). GTX629448

GTX629448 IHC-P Image

5-Methylcytosine / 5-mC antibody [GT4111] detects 5-Methylcytosine / 5-mC protein at nucleus on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
5-Methylcytosine / 5-mC antibody [GT4111] (GTX629448) dilution: 1:200.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-5-Methylcytosine / 5-mC antibody [GT4111] used in IHC (Paraffin sections) (IHC-P). GTX629448

GTX629448 IHC-P Image

5-Methylcytosine / 5-mC antibody [GT4111] detects 5-Methylcytosine / 5-mC protein at nucleus on mouse urinary bladder by immunohistochemical analysis.
Sample: Paraffin-embedded mouse urinary bladder.
5-Methylcytosine / 5-mC antibody [GT4111] (GTX629448) dilution: 1:200.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-5-Methylcytosine / 5-mC antibody [GT4111] used in IHC (Paraffin sections) (IHC-P). GTX629448

GTX629448 IHC-P Image

5-Methylcytosine / 5-mC antibody [GT4111] detects 5-Methylcytosine / 5-mC protein at nucleolus in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
5-Methylcytosine / 5-mC antibody [GT4111] (GTX629448) diluted at 1:200.

REFERENCE
REVIEW

5-Methylcytosine / 5-mC antibody [GT4111] Cat No. GTX629448

Rating ( Average 4.5 based on 2 users reviews)
Application
IHC (Paraffin sections)(IHC-P) ( Average 5 based on 1 users reviews)
Dot blot(Dot) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 15-Dec-2015
Score :
Application Tested : IHC-P
Sample : endometrium tissue
Blocking : commercial blocking buffer, 25°C, 1Hr
Fixation : 4 % paraformaldehyde in PBS
Permeabilization /Antigen retrieval : 0.2 % Triton X-100 in PBS/ citrate buffer (10 mM, pH 6.0)
Primary Antibody : 1:200, 4°C, 16Hr
Date : Anonymous submitted on 07-Mar-2014
Score :
Application Tested : Dot
Sample : SH-SY5Y genomic DNA
Amount used : 2 μg of chromosomal DNA
Blocking : 5% milk in PBST (1x PBS + 0,05% Tween-20), 25°C, 1Hr
Primary Antibody : 1:500, 25°C, 1Hr
SDS
PBS.pdf
Package List Price ($)
$ 319
$ 169