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5-Methylcytosine / 5-mC antibody [GT4111]

Anti-5-Methylcytosine / 5-mC antibody [GT4111] used in Methylated DNA Immunoprecipitation (MeDIP). GTX629448
Anti-5-Methylcytosine / 5-mC antibody [GT4111] used in Dot blot (Dot). GTX629448
Anti-5-Methylcytosine / 5-mC antibody [GT4111] used in IHC (Whole mount) (IHC-Wm). GTX629448
Anti-5-Methylcytosine / 5-mC antibody [GT4111] used in IHC (Paraffin sections) (IHC-P). GTX629448
Anti-5-Methylcytosine / 5-mC antibody [GT4111] used in IHC (Paraffin sections) (IHC-P). GTX629448
Anti-5-Methylcytosine / 5-mC antibody [GT4111] used in IHC (Whole mount) (IHC-Wm). GTX629448
Anti-5-Methylcytosine / 5-mC antibody [GT4111] used in IHC (Paraffin sections) (IHC-P). GTX629448
Anti-5-Methylcytosine / 5-mC antibody [GT4111] used in IHC (Paraffin sections) (IHC-P). GTX629448

Cat. No. GTX629448

Reference 10
2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT4111

Isotype

 IgG1

Application

 IHC-P, IHC-Fr, IHC-Wm, Dot, EM, MeDIP

Reactivity

 Species independent
Package
100 μl ($399),
25 μl ($169)
See all 5-Methylcytosine / 5-mC products
PRODUCT

Summary

5-mC antibody detects methylated cytosine, which is a key epigenetic regulatory modification of genomic DNA and is considered a “fifth base”. The 5-mC mark is added by DNA methyltransferases (DNMTs) and serves predominately to turn off gene transcription. It is reversible via a DNA demethylation pathway triggered by the Ten-eleven translocation (TET) family of dioxygenases.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
IHC-Wm 1:50-1:500
Dot Assay dependent
EM Assay dependent
MeDIP Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.7 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to 5-Methylcytosine / 5-mC

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888114

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-5-Methylcytosine / 5-mC antibody [GT4111] used in Methylated DNA Immunoprecipitation (MeDIP). GTX629448

GTX629448 MeDIP Image

GTX629448 5-Methylcytosine/ 5-mC antibody [GT4111] in MeDIP experiment.
Human genomic DNA (500 ng) mixed with methylated cytosine standard kit (GTX400004) were subjected to MeDIP with 5-Methylcytosine/ 5-mC antibody [GT4111] (GTX629448) at dilution of 1:500 and analyzed with semi-quantitative PCR. The immunoprecipitated DNA was plotted as % of input DNA.

Anti-5-Methylcytosine / 5-mC antibody [GT4111] used in Dot blot (Dot). GTX629448

GTX629448 Dot Image

GTX629448 5-methylcytosine antibody [GT4111] Dot blot analysis of anti-5-mC antibody with the synthetic DNA controls (GTX400004). DNA samples (0.5 to 50 ng) were spotted onto positively charged nylon membrane and blotted with 5-mC antibody (GTX629448) at a dilution of 1:500. A: Unmethylated DNA fragment B: DNA fragment containing 5-methylcytosine C: DNA fragment containing 5-hydroxymethylcytosine D: DNA fragment containing 5-formylcytosine E: DNA fragment containing 5-carboxylcytosine

Anti-5-Methylcytosine / 5-mC antibody [GT4111] used in IHC (Whole mount) (IHC-Wm). GTX629448

GTX629448 IHC-Wm Image

5-Methylcytosine / 5-mC antibody [GT4111] detects 5-Methylcytosine / 5-mC protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 24 hours-post-fertilization zebrafish embryo.
5-Methylcytosine / 5-mC antibody [GT4111] (GTX629448) dilution: 1:50.
Anti-5-Methylcytosine / 5-mC antibody [GT4111] used in IHC (Paraffin sections) (IHC-P). GTX629448

GTX629448 IHC-P Image

5-Methylcytosine / 5-mC antibody [GT4111] detects 5-Methylcytosine / 5-mC protein at nucleolus in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
5-Methylcytosine / 5-mC antibody [GT4111] (GTX629448) diluted at 1:200.
Anti-5-Methylcytosine / 5-mC antibody [GT4111] used in IHC (Paraffin sections) (IHC-P). GTX629448

GTX629448 IHC-P Image

5-Methylcytosine / 5-mC antibody [GT4111] detects 5-Methylcytosine / 5-mC protein at nucleus on mouse urinary bladder by immunohistochemical analysis.
Sample: Paraffin-embedded mouse urinary bladder.
5-Methylcytosine / 5-mC antibody [GT4111] (GTX629448) dilution: 1:200.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-5-Methylcytosine / 5-mC antibody [GT4111] used in IHC (Whole mount) (IHC-Wm). GTX629448

GTX629448 IHC-Wm Image

5-Methylcytosine / 5-mC antibody [GT4111] detects 5-Methylcytosine / 5-mC protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 24 hours-post-fertilization zebrafish embryo.
5-Methylcytosine / 5-mC antibody [GT4111] (GTX629448) dilution: 1:50.
Anti-5-Methylcytosine / 5-mC antibody [GT4111] used in IHC (Paraffin sections) (IHC-P). GTX629448

GTX629448 IHC-P Image

5-Methylcytosine / 5-mC antibody [GT4111] detects 5-Methylcytosine / 5-mC protein at nucleus on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
5-Methylcytosine / 5-mC antibody [GT4111] (GTX629448) dilution: 1:200.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-5-Methylcytosine / 5-mC antibody [GT4111] used in IHC (Paraffin sections) (IHC-P). GTX629448

GTX629448 IHC-P Image

5-Methylcytosine / 5-mC antibody [GT4111] detects 5-Methylcytosine / 5-mC protein at nucleus on human ovarian carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian carcinoma.
5-Methylcytosine / 5-mC antibody [GT4111] (GTX629448) dilution: 1:200.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

C Casali et al. Eur J Cell Biol 2023; 103 (1) : 151373 Impact of heat and cold shock on epigenetics and chromatin structure
Application : EM
Lacavalla MA et al. Eur J Histochem 2021; 65 (2) Ultrastructural immunocytochemistry shows impairment of RNA pathways in skeletal muscle nuclei of old mice: A link to sarcopenia?
Chastain-Potts SE et al. Mol Neurobiol 2019; Sevoflurane Exposure Results in Sex-Specific Transgenerational Upregulation of Target IEGs in the Subiculum.
Application : IHC-P
Wu J et al. J Exp Clin Cancer Res 2019; 38 (1) : 348 TET1-mediated DNA hydroxymethylation activates inhibitors of the Wnt/帣-catenin signaling pathway to suppress EMT in pancreatic tumor cells.
Application : Dot
Kong X et al. Brain Behav Immun 2019; (Epub) JAK2/STAT3 signaling mediates IL-6-inhibited neurogenesis of neural stem cells through DNA demethylation/methylation.
Application : IHC-Fr
Show More +
Submit a Reference

REVIEW

5-Methylcytosine / 5-mC antibody [GT4111] Cat. No. GTX629448

Rating
( Average 4.5 based on 2 users reviews)
Application
IHC (Paraffin sections)(IHC-P)
( Average 5 based on 1 users reviews)
Dot blot(Dot)
( Average 4 based on 1 users reviews)
Anti-5-Methylcytosine / 5-mC antibody [GT4111] used in IHC (Paraffin sections) (IHC-P). GTX629448
Date : Anonymous submitted on 15-Dec-2015
Score :
Application Tested : IHC-P
Sample : endometrium tissue
Blocking : commercial blocking buffer, 25°C, 1Hr
Fixation : 4 % paraformaldehyde in PBS
Permeabilization /Antigen retrieval : 0.2 % Triton X-100 in PBS/ citrate buffer (10 mM, pH 6.0)
Primary Antibody : 1:200, 4°C, 16Hr
Anti-5-Methylcytosine / 5-mC antibody [GT4111] used in Dot blot (Dot). GTX629448
Date : Anonymous submitted on 07-Mar-2014
Score :
Application Tested : Dot
Sample : SH-SY5Y genomic DNA
Amount used : 2 μg of chromosomal DNA
Blocking : 5% milk in PBST (1x PBS + 0,05% Tween-20), 25°C, 1Hr
Primary Antibody : 1:500, 25°C, 1Hr
Submit a Review
Application Reference
C Casali et al. Eur J Cell Biol 2023; 103 (1):151373 Impact of heat and cold shock on epigenetics and chromatin structure
Application : EM
Lacavalla MA et al. Eur J Histochem 2021; 65 (2) Ultrastructural immunocytochemistry shows impairment of RNA pathways in skeletal muscle nuclei of old mice: A link to sarcopenia?
Chastain-Potts SE et al. Mol Neurobiol 2019; Sevoflurane Exposure Results in Sex-Specific Transgenerational Upregulation of Target IEGs in the Subiculum.
Application : IHC-P
Wu J et al. J Exp Clin Cancer Res 2019; 38 (1):348 TET1-mediated DNA hydroxymethylation activates inhibitors of the Wnt/帣-catenin signaling pathway to suppress EMT in pancreatic tumor cells.
Application : Dot
Kong X et al. Brain Behav Immun 2019; (Epub) JAK2/STAT3 signaling mediates IL-6-inhibited neurogenesis of neural stem cells through DNA demethylation/methylation.
Application : IHC-Fr
Cesar S.Huertas et al. Biosensors and Bioelectronics 2018; 120:47-54 Label-free DNA-methylation detection by direct ds-DNA fragment screening using poly-purine hairpins.
Masiello I et al. Cell Mol Life Sci 2017; (Epub) Ultrastructural localization of 5-methylcytosine on DNA and RNA.
Application : EM
Hsieh MC et al. Sci Rep 2016; 6:37411 Tet1-dependent epigenetic modification of BDNF expression in dorsal horn neurons mediates neuropathic pain in rats.
Application : Dot
Shih CC et al. PLoS One 2016; 11 (9):e0163690 Procainamide Inhibits DNA Methylation and Alleviates Multiple Organ Dysfunction in Rats with Endotoxic Shock.
Application : IHC-P
Hsiao KY et al. Mol Hum Reprod 2015; (Epub) Coordination of AUF1 and miR-148a destabilizes DNA methyltransferase 1 mRNA under hypoxia in endometriosis.
Application : IHC-P
SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations