5-Methylcytosine / 5-mC antibody [GT9076]

ICC/IF analysis of 4% paraformaldehyde fixed HeLa cells using GTX60803 5-Methylcytosine / 5-mC antibody [GT9076].
Red : Primary antibody
Blue : DAPI
Dilution : 1:1000

ICC/IF analysis of 4% PFA-fixed mouse embryos cells fixed in the one-cellular stage (after blocking with PBS containing 2% BSA, the cells were treated with 2N HCl) using GTX60803 5-Methylcytosine / 5-mC antibody [GT9076]. Both the maternal (M) and paternal (P) pronuclei are stained.
Left : Primary antibody
Right : Anti- single stranded DNA antibody
Permeabilization : 0.5% Triton X-100 for 30 mins
Dilution : 1:200

FISH (Fluorescence in situ hybridization) analysis of metaphase chromosomes from HeLa cells (treated with 0.1 μg/ml colcemid for 1 - 2 hours) using GTX60803 5-Methylcytosine / 5-mC antibody [GT9076].
Red : Primary antibody
Blue : DAPI
Dilution : 1:1000

Dot blot analysis of 4 - 100 ng (equivalent of 5 to 0.2 pmol of C-bases) hmC, mC and C controls using GTX60803 5-Methylcytosine / 5-mC antibody [GT9076].
Dilution : 1:600

ICC/IF analysis of 2.5% PFA fixed U2OS cells using GTX60803 5-Methylcytosine / 5-mC antibody [GT9076].
Figure A : cells were immunofluorescently labelled with the 5-mC antibody after incubation of the antibody with 50 μM mCTP (left) or with DAPI (right).
Figure B : staining of the cells with the 5-mC antibody after incubation of the antibody with 50 μM hmCTP and with DAPI.
Dilution : 1:500