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5-Methylcytosine / 5-mC antibody [RM231]

Anti-5-Methylcytosine / 5-mC antibody [RM231] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33606
Anti-5-Methylcytosine / 5-mC antibody [RM231] used in ELISA (ELISA). GTX33606
Anti-5-Methylcytosine / 5-mC antibody [RM231] used in Dot blot (Dot). GTX33606

Cat No. GTX33606

Host

Rabbit

Clonality

Monoclonal

Clone Name

RM231

Isotype

IgG

Application

ICC/IF, Dot, ELISA, IHC, MeDIP
Package
50 μg ($349)

APPLICATION

Application Note

Dot Blot: 0.5 ug/mL – 2 ug/mL, ELISA: 0.1 ug/mL - 1 ug/mL, ICC: 0.5 ug/mL - 2 ug/mL.

Specificity/Sensitivity

This antibody reacts to 5-methylcytosine in both single-stranded and double-stranded DNA. No cross reactivity with non-methylated cytosine and hydroxymethylcytosine in DNA.

PROPERTIES

Form

Liquid

Buffer

50% Glycerol/PBS with 1% BSA and 0.09% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

BSA-conjugated 5-methylcytosine.

Purification

Protein A affinity purified from an animal origin-free culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

DATA IMAGES

Anti-5-Methylcytosine / 5-mC antibody [RM231] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33606

GTX33606 ICC/IF Image

Immunocytochemical staining of HeLa cells using rabbit monoclonal anti-5-mC (clone RM231) antibody (red). Actin filaments have been labeled with fluorescein phalloidin (green), and nuclei stained with DAPI (blue).

Anti-5-Methylcytosine / 5-mC antibody [RM231] used in ELISA (ELISA). GTX33606

GTX33606 ELISA Image

ELISA of single stranded DNA using rabbit monoclonal anti-5-mC (clone RM231) antibody. The plate was coatedwith streptavidin and then biotinylated single stranded unmethylated DNA, 5-Methylcytosine (5-mC) DNA, and 5-Hydroxymethylcytosine (5-hmC) DNA. A serial dilution of RM231 was used as the primary antibody, and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

Anti-5-Methylcytosine / 5-mC antibody [RM231] used in Dot blot (Dot). GTX33606

GTX33606 Dot Image

Dot blot of double stranded DNA using rabbit monoclonal anti-5-mC (clone RM231) antibody. The membrane waspre-spotted with 50, 5, and 0.5 ng/dot of double stranded 5-Hydroxymethylcytosine (5-hmC) DNA, 5-Methylcytosine (5-mC) DNA, and unmethylated DNA. The pre-spotted membrane was then blotted with RM231.

REFERENCE

REVIEW

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Package List Price ($)
$ 349