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5-Methylcytosine / 5-mC antibody [RM231]

Anti-5-Methylcytosine / 5-mC antibody [RM231] used in Dot blot (Dot). GTX33606
Anti-5-Methylcytosine / 5-mC antibody [RM231] used in ELISA (ELISA). GTX33606
Anti-5-Methylcytosine / 5-mC antibody [RM231] used in ELISA (ELISA). GTX33606
Anti-5-Methylcytosine / 5-mC antibody [RM231] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33606
Anti-5-Methylcytosine / 5-mC antibody [RM231] used in Flow cytometry (FACS). GTX33606
Anti-5-Methylcytosine / 5-mC antibody [RM231] used in Methylated DNA Immunoprecipitation (MeDIP). GTX33606

Cat No. GTX33606

Host

Rabbit

Clonality

Monoclonal

Clone Name

RM231

Isotype

IgG

Application

ICC/IF, FACS, Dot, ELISA, IHC, MeDIP
Package
50 μg ($429)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 0.5 μg/mL - 2 μg/mL
FACS 0.5 μg/mL - 2 μg/mL
Dot 0.5 μg/mL - 2 μg/mL
ELISA 0.1 μg/mL - 1 μg/mL
IHC Assay dependent
MeDIP 0.2 μg/mL - 2 μg/mL
Not tested in other applications.

Specificity/Sensitivity

This antibody reacts to 5-methylcytosine in both single-stranded and double-stranded DNA. No cross reactivity with non-methylated cytosine and hydroxymethylcytosine in DNA.

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 0.09% sodium azide, 50% Glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Immunogen

BSA-conjugated 5-methylcytosine.

Purification

Protein A purified
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

DATA IMAGES

Anti-5-Methylcytosine / 5-mC antibody [RM231] used in Dot blot (Dot). GTX33606

GTX33606 Dot Image

Dot blot analysis of 50, 5, and 0.5 ng/dot of double stranded 5-Hydroxymethylcytosine (5-hmC) DNA, 5-Methylcytosine (5-mC) DNA, and unmethylated DNA using GTX33606 5-Methylcytosine / 5-mC antibody [RM231].

Anti-5-Methylcytosine / 5-mC antibody [RM231] used in ELISA (ELISA). GTX33606

GTX33606 ELISA Image

ELISA analysis of HeLa cell genomic DNA using GTX33606 5-Methylcytosine / 5-mC antibody [RM231].
Dilution : 1 ug/mL or 3 ug/mL of RM231 was used as the primary antibody.

Anti-5-Methylcytosine / 5-mC antibody [RM231] used in ELISA (ELISA). GTX33606

GTX33606 ELISA Image

ELISA analysis of unmethylated DNA, 5-Methylcytosine (5-mC) DNA, and 5-Hydroxymethylcytosine (5-hmC) DNA using serial diluted GTX33606 5-Methylcytosine / 5-mC antibody [RM231].

Anti-5-Methylcytosine / 5-mC antibody [RM231] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33606

GTX33606 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX33606 5-Methylcytosine / 5-mC antibody [RM231].
Red : Primary antibody
Green : Actin
Blue : DAPI

Anti-5-Methylcytosine / 5-mC antibody [RM231] used in Flow cytometry (FACS). GTX33606

GTX33606 FACS Image

FACS analysis of HEK293 cells using GTX33606 5-Methylcytosine / 5-mC antibody [RM231]. The cells were fixed with ice-cold MeOH, permeabilized with 0.5% Triton X-100, denatured with 2N HCl, then stained with GTX33606 (Blue) or with a negative control PEG antibody (Red).

Anti-5-Methylcytosine / 5-mC antibody [RM231] used in Methylated DNA Immunoprecipitation (MeDIP). GTX33606

GTX33606 MeDIP Image

MeDIP analysis of 1 ng of unmethylated, 5-Methylcytosine (5-mC) or 5-Hydroxymethylcytosine (5-hmC) DNA standard (897 bp) spiked in 1ug of genomic DNA isolated from HeLa cells using GTX33606 5-Methylcytosine / 5-mC antibody [RM231]. MeDIP was performed at a 10:1 DNA:Ab ratio. Realtime PCR was then performed to determine the capture of DNA standard as in % of input.

REFERENCE

REVIEW

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Package List Price ($)
$ 429