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5-Methylcytosine / 5-mC antibody

Anti-5-Methylcytosine / 5-mC antibody used in Methylated DNA Immunoprecipitation (MeDIP). GTX128455
Anti-5-Methylcytosine / 5-mC antibody used in Dot blot (Dot). GTX128455

Cat No. GTX128455

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application Dot, EM, MeDIP
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
Dot Assay dependent
EM Assay dependent
MeDIP Assay dependent
Not tested in other applications.
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

7.65 mg/ml (Please refer to the vial label for the specific concentration.)

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
DATA IMAGES
Anti-5-Methylcytosine / 5-mC antibody used in Methylated DNA Immunoprecipitation (MeDIP). GTX128455

GTX128455 MeDIP Image

GTX128455 5-Methylcytosine/ 5-mC antibody in MeDIP experiment.
Human genomic DNA (150 ng) mixed with methylated cytosine standard kit (GTX400004) were subjected to MeDIP with 5-Methylcytosine/ 5-mC antibody (GTX128455) at dilution of 1:2000 and analyzed with semi-quantitative PCR. The immunoprecipitated DNA was plotted as % of input DNA.

Anti-5-Methylcytosine / 5-mC antibody used in Dot blot (Dot). GTX128455

GTX128455 Dot Image

GTX128455 5-methylcytosine antibody Dot blot analysis of anti-5-mC antibody with the synthetic DNA controls (GTX400004). DNA samples (0.5 to 50 ng) were spotted onto positively charged nylon membrane and blotted with 5-mC antibody (GTX128455) at a dilution of 1:4000.
A: Unmethylated DNA
B: DNA containing 5-methylcytosine
C: DNA containing 5-hydroxymethylcytosine
D: DNA containing 5-formylcytosine
E: DNA containing 5-carboxylcytosine

REFERENCE
REVIEW

5-Methylcytosine / 5-mC antibody Cat No. GTX128455

Rating ( Average 5 based on 1 users reviews)
Application
Electron microscopy(EM) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 25-Jul-2016
Score :
Application Tested : EM
Sample : Rat liver tissue
Blocking : Normal Goat Serum in PBS, 25°C, 0.08Hr
Fixation : 4% PFA in S?rensen phosphate buffer (pH 7.2)
Permeabilization /Antigen retrieval : 0.1% BSA in PBST
Primary Antibody : 1:500, 4°C, 16Hr
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169