5-Methylcytosine / 5-mC antibody

Name: 5-Methylcytosine / 5-mC antibody
Brand: GeneTex
SKU: GTX128455
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-5-Methylcytosine / 5-mC antibody used in Methylated DNA Immunoprecipitation (MeDIP). GTX128455

Anti-5-Methylcytosine / 5-mC antibody used in Dot blot (Dot). GTX128455

Cat. No. GTX128455

Reference 2

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	Dot, EM, MeDIP
Reactivity	Species independent
Package	100 μl ($399), 25 μl ($169)

See all 5-Methylcytosine / 5-mC products

View product citations for antibody GTX128455 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
Dot	Assay dependent
EM	Assay dependent
MeDIP	Assay dependent

Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

7.55 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to 5-MethylCytosine / 5-mC

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885780

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

GTX128455 MeDIP Image

GTX128455 5-Methylcytosine/ 5-mC antibody in MeDIP experiment.
Human genomic DNA (150 ng) mixed with methylated cytosine standard kit (GTX400004) were subjected to MeDIP with 5-Methylcytosine/ 5-mC antibody (GTX128455) at dilution of 1:2000 and analyzed with semi-quantitative PCR. The immunoprecipitated DNA was plotted as % of input DNA.

GTX128455 Dot Image

GTX128455 5-methylcytosine antibody Dot blot analysis of anti-5-mC antibody with the synthetic DNA controls (GTX400004). DNA samples (0.5 to 50 ng) were spotted onto positively charged nylon membrane and blotted with 5-mC antibody (GTX128455) at a dilution of 1:4000.
A: Unmethylated DNA
B: DNA containing 5-methylcytosine
C: DNA containing 5-hydroxymethylcytosine
D: DNA containing 5-formylcytosine
E: DNA containing 5-carboxylcytosine

REFERENCE

Application Reference

Zannino L et al. Methods Mol Biol 2020; 2175 : 197-205 Timing of Cytosine Methylation on Newly Synthesized RNA by Electron Microscopy.

Masiello I et al. Cell Mol Life Sci 2017; (Epub) Ultrastructural localization of 5-methylcytosine on DNA and RNA.

Application : EM

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