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53BP1 antibody [HL275]

Anti-53BP1 antibody [HL275] used in Western Blot (WB). GTX635479
Anti-53BP1 antibody [HL275] used in Western Blot (WB). GTX635479
Anti-53BP1 antibody [HL275] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635479
Anti-53BP1 antibody [HL275] used in IHC (Paraffin sections) (IHC-P). GTX635479
Anti-53BP1 antibody [HL275] used in Western Blot (WB). GTX635479
Anti-53BP1 antibody [HL275] used in Western Blot (WB). GTX635479
Anti-53BP1 antibody [HL275] used in Western Blot (WB). GTX635479

Cat No. GTX635479

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL275

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

214 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.42 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human 53BP1. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888536

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

tumor protein p53 binding protein 1 , 53BP1 , TDRD30 , p202 , p53BP1

Cellular Localization

Nucleus

Background

This gene encodes a protein that functions in the DNA double-strand break repair pathway choice, promoting non-homologous end joining (NHEJ) pathways, and limiting homologous recombination. This protein plays multiple roles in the DNA damage response, including promoting checkpoint signaling following DNA damage, acting as a scaffold for recruitment of DNA damage response proteins to damaged chromatin, and promoting NHEJ pathways by limiting end resection following a double-strand break. These roles are also important during V(D)J recombination, class switch recombination and at unprotected telomeres. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-53BP1 antibody [HL275] used in Western Blot (WB). GTX635479

GTX635479 WB Image

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with 53BP1 antibody [HL36] (GTX635479) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-53BP1 antibody [HL275] used in Western Blot (WB). GTX635479

GTX635479 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with 53BP1 antibody [HL36] (GTX635479) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-53BP1 antibody [HL275] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635479

GTX635479 ICC/IF Image

53BP1 antibody [HL275] detects 53BP1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: 53BP1 stained by 53BP1 antibody [HL275] (GTX635479) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10 μm.

Anti-53BP1 antibody [HL275] used in IHC (Paraffin sections) (IHC-P). GTX635479

GTX635479 IHC-P Image

53BP1 antibody [HL275] detects 53BP1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
53BP1 stained by 53BP1 antibody [HL275] (GTX635479) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-53BP1 antibody [HL275] used in Western Blot (WB). GTX635479

GTX635479 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with 53BP1 antibody [HL36] (GTX635479) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-53BP1 antibody [HL275] used in Western Blot (WB). GTX635479

GTX635479 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with 53BP1 antibody [HL36] (GTX635479) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-53BP1 antibody [HL275] used in Western Blot (WB). GTX635479

GTX635479 WB Image

HeLa whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with 53BP1 antibody [HL36] (GTX635479) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 379
$ 169
Custom Conjugations