GeneTex
United States (US)

53BP1 antibody [N1], N-term

KO/KD
GTX102595_39848_20161103_WB_shRNA_watermark_19011506_823.jpg
GTX102595_39848_20160128_WB_M_19011506_771.jpg
GTX102595_39848_WB_19011506_961.jpg
GTX102595_39848_IHC_19011506_892.jpg
GTX102595_39848_20150410_IFA_19011506_693.jpg

Cat No. GTX102595

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

214 kDa. ( Note )

Positive Control

293T , HeLa , Neuro 2A , C8D30 , NIH-3T3
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human 53BP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

53BP1 antibody, FLJ41424 antibody, MGC138366 antibody, p202 antibody, TP53BP1 antibody, tumor suppressor p53-binding protein 1 antibody, tumor protein 53-binding protein, 1 antibody, tumor protein p53-binding protein, 1 antibody, p53BP1 antibody, p53-binding protein 1 antibody, tumor protein p53 binding protein 1 antibody

Cellular Localization

Nucleus , Kinetochore

Background

May have a role in checkpoint signaling during mitosis (By similarity). Enhances TP53-mediated transcriptional activation. Plays a role in the response to DNA damage.

Database

Research Area

DATA IMAGES
GTX102595_39848_20161103_WB_shRNA_watermark_19011506_823.jpg

GTX102595 WB Image

KO/KD

Non-transfected (–) and transfected (+) HeLa whole cell extracts (50 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with 53BP1 antibody [N1], N-term (GTX102595) diluted at 1:500.

GTX102595_39848_20160128_WB_M_19011506_771.jpg

GTX102595 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with 53BP1 antibody [N1], N-term (GTX102595) diluted at 1:500.

GTX102595_39848_WB_19011506_961.jpg

GTX102595 WB Image

53BP1 antibody [N1], N-term detects TP53BP1 protein by Western blot analysis.
A. 30 µg 293T whole cell lysate/extract
B. 30 µg HeLa whole cell lysate/extract
5 % SDS-PAGE
53BP1 antibody [N1], N-term (GTX102595) dilution: 1:500

GTX102595_39848_IHC_19011506_892.jpg

GTX102595 IHC-P Image

53BP1 antibody [N1], N-term detects 53BP1 protein at nucleus on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
53BP1 antibody [N1], N-term (GTX102595) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX102595_39848_20150410_IFA_19011506_693.jpg

GTX102595 ICC/IF Image

53BP1 antibody [N1], N-term detects 53BP1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: 53BP1 protein stained by 53BP1 antibody [N1], N-term (GTX102595) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000.

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf