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53BP1 antibody [N1], N-term

KO/KD
Anti-53BP1 antibody [N1], N-term used in Western Blot (WB). GTX102595
Anti-53BP1 antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102595
Anti-53BP1 antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX102595
Anti-53BP1 antibody [N1], N-term used in Western Blot (WB). GTX102595
Anti-53BP1 antibody [N1], N-term used in Western Blot (WB). GTX102595

Cat. No. GTX102595

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all 53BP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

214 kDa. ( Note )

Positive Control

293T , HeLa , Neuro 2A , C8D30 , NIH-3T3

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human 53BP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036128

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tumor protein p53 binding protein 1 , 53BP1 , TDRD30 , p202 , p53BP1

Cellular Localization

Nucleus , Kinetochore

Background

May have a role in checkpoint signaling during mitosis (By similarity). Enhances TP53-mediated transcriptional activation. Plays a role in the response to DNA damage.

Database

Research Area

DATA IMAGES

Anti-53BP1 antibody [N1], N-term used in Western Blot (WB). GTX102595

GTX102595 WB Image

KO/KD

Non-transfected (–) and transfected (+) HeLa whole cell extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with 53BP1 antibody [N1], N-term (GTX102595) diluted at 1:500.

Anti-53BP1 antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102595

GTX102595 ICC/IF Image

53BP1 antibody [N1], N-term detects 53BP1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: 53BP1 protein stained by 53BP1 antibody [N1], N-term (GTX102595) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000.
Anti-53BP1 antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX102595

GTX102595 IHC-P Image

53BP1 antibody [N1], N-term detects 53BP1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
53BP1 stained by 53BP1 antibody [N1], N-term (GTX102595) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-53BP1 antibody [N1], N-term used in Western Blot (WB). GTX102595

GTX102595 WB Image

53BP1 antibody [N1], N-term detects TP53BP1 protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg HeLa whole cell lysate/extract
5 % SDS-PAGE
53BP1 antibody [N1], N-term (GTX102595) dilution: 1:500

Anti-53BP1 antibody [N1], N-term used in Western Blot (WB). GTX102595

GTX102595 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with 53BP1 antibody [N1], N-term (GTX102595) diluted at 1:500.

REFERENCE

Application Reference

Uyeda A et al. J Neurosci 2020; 40 (47) : 9012-9027 Suppression of DNA Double-Strand Break Formation by DNA Polymerase β in Active DNA Demethylation Is Required for Development of Hippocampal Pyramidal Neurons.
Application : IHC-Fr
Reactivity : Mouse
Onishi K et al. J Neurosci 2017; (Epub) Genome Stability by DNA polymerase β in Neural Progenitors Contributes to Neuronal Differentiation in Cortical Development.
Application : WB
Reactivity : Mouse
Chang HY et al. PLoS One 2013; 8 (1) : e54117 Middle infrared radiation induces G2/M cell cycle arrest in A549 lung cancer cells.
Application : ICC/IF
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169