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53BP1 antibody

Anti-53BP1 antibody used in Western Blot (WB). GTX30658
Anti-53BP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX30658
Anti-53BP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30658

Cat. No. GTX30658

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP, PLA

Reactivity

 Human, Mouse
Package
100 μl ($449)
See all 53BP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000 - 1:10000
ICC/IF 1:100 - 1:1000
IHC-P 1:400
IP Assay dependent
PLA Assay dependent
Not tested in other applications.

Calculated MW

214 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a portion of human 53BP1 encoded in exon 11, 12 and 19 [UniProt# Q12888]. This antibody is a cocktail hybrid of NB100-304 and NB100-305.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_625767

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tumor protein p53 binding protein 1 , 53BP1 , TDRD30 , p202 , p53BP1

Cellular Localization

Nucleus

Background

May have a role in checkpoint signaling during mitosis (By similarity). Enhances TP53-mediated transcriptional activation. Plays a role in the response to DNA damage.

Database

Research Area

DATA IMAGES

Anti-53BP1 antibody used in Western Blot (WB). GTX30658

GTX30658 WB Image

WB analysis of U2OS cell lysate using GTX30658 53BP1 antibody.

Anti-53BP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX30658

GTX30658 IHC-P Image

IHC-P analysis of human renal carcinoma tissue using GTX30658 53BP1 antibody.

Anti-53BP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30658

GTX30658 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX30658 53BP1 antibody.
Green : primary antibody
Red : Tubulin
Blue : DAPI

REFERENCE

Application Reference

Chen X et al. Carcinogenesis 2017; : 43110 Dicer regulates non-homologous end joining and is associated with chemosensitivity in colon cancer patients.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 449