GeneTex
United States (US)

53BP1 antibody

GTX30658_1464_WB_18121410_305.jpg
GTX30658_335_IHC-P_18121410_882.jpg
GTX30658_225_ICC-IF_18121410_292.jpg

Cat No. GTX30658

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP, PLA
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000 - 1:10000
ICC/IF 1:100 - 1:1000
IHC-P 1:400
IP Assay dependent
PLA Assay dependent
Not tested in other applications.

Calculated MW

214 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS, 0.02% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a portion of human 53BP1 encoded in exon 11, 12 and 19 [UniProt# Q12888]. This antibody is a cocktail hybrid of NB100-304 and NB100-305.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

53BP1 antibody , TDRD30 antibody , TP53 antibody , p202 antibody , p53BP1 antibody

Cellular Localization

Nucleus,Kinetochore

Background

May have a role in checkpoint signaling during mitosis (By similarity). Enhances TP53-mediated transcriptional activation. Plays a role in the response to DNA damage.

Database

Research Area

DATA IMAGES
GTX30658_1464_WB_18121410_305.jpg

GTX30658 WB Image

WB analysis of U2OS cell lysate using GTX30658 53BP1 antibody.

GTX30658_335_IHC-P_18121410_882.jpg

GTX30658 IHC-P Image

IHC-P analysis of human renal carcinoma tissue using GTX30658 53BP1 antibody.

GTX30658_225_ICC-IF_18121410_292.jpg

GTX30658 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX30658 53BP1 antibody.
Green : primary antibody
Red : Tubulin
Blue : DAPI

REFERENCE