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53BP1 antibody

Anti-53BP1 antibody used in Western Blot (WB). GTX70310
Anti-53BP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX70310
Anti-53BP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX70310
Anti-53BP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX70310
Anti-53BP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70310
Anti-53BP1 antibody used in Western Blot (WB). GTX70310
Anti-53BP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX70310
Anti-53BP1 antibody used in Western Blot (WB). GTX70310

Cat No. GTX70310

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC

Reactivity

Human, Mouse
Package
100 μl ($299)

APPLICATION

Application Note

Suggested starting dilutions are as follows: WB: 1:500-1:3000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Calculated MW

214 kDa. ( Note )

Positive Control

HeLa , *HaCaT

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

5.08 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to the carboxy-terminus of h53BP1, conjugated to a carrier protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

tumor protein p53 binding protein 1 , 53BP1 , TDRD30 , p202 , p53BP1

Background

P53-binding protein 1 (53BP1) plays a critical role in tumor suppression and is a putative substrate of ATM kinase. Upon DNA damage, it is phosphorylated and relocalizes to the presumptive sites of damage, specifically, double-strand breaks. This also suggests a role in DNA repair, maintaining genomic stability.

Database

Research Area

DATA IMAGES

Anti-53BP1 antibody used in Western Blot (WB). GTX70310

GTX70310 WB Image

Non-transfected (–) and transfected (+) HeLa whole cell extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with 53BP1 antibody (GTX70310) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-53BP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX70310

GTX70310 IHC-P Image

53BP1 antibody detects 53BP1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
53BP1 stained by 53BP1 antibody (GTX70310) diluted at 1:4000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-53BP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX70310

GTX70310 IHC-P Image

53BP1 antibody detects 53BP1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
53BP1 stained by 53BP1 antibody (GTX70310) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-53BP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX70310

GTX70310 IHC-P Image

53BP1 antibody detects 53BP1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
53BP1 stained by 53BP1 antibody (GTX70310) diluted at 1:4000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-53BP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70310

GTX70310 ICC/IF Image

53BP1 antibody detects 53BP1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: 53BP1 stained by 53BP1 antibody (GTX70310) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.
Scale bar= 10 μm.

Anti-53BP1 antibody used in Western Blot (WB). GTX70310

GTX70310 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with 53BP1 antibody (GTX70310) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-53BP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX70310

GTX70310 IHC-P Image

53BP1 antibody detects 53BP1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
53BP1 stained by 53BP1 antibody (GTX70310) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-53BP1 antibody used in Western Blot (WB). GTX70310

GTX70310 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with 53BP1 antibody (GTX70310) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

53BP1 antibody Cat No. GTX70310

Rating ( Average 4 based on 1 users reviews)
Application
Western Blot(WB) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 12-Sep-2013
Score :
Application Tested : WB
Sample Species : Hu
Sample : HaCaT cells
Amount used : 50μg
Blocking : 5% milk, 20°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf