58K Golgi protein antibody [58K-9]

Name: 58K Golgi protein antibody [58K-9]
Brand: GeneTex
SKU: GTX26284
Price: 409.00 USD
Rating: 5 (1 reviews)

Cat. No. GTX26284

Reference 5

Host	Mouse
Clonality	Monoclonal
Clone Name	58K-9
Isotype	IgG1
Application	WB, ICC/IF, IHC-P, IP, Dot, EM
Reactivity	Human, Mouse, Rat, Bovine, Dog, Hamster, Pig, Monkey, Kangaroo rat
Package	100 μl ($409)

See all 58K Golgi protein products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:5,000
ICC/IF	1:50
IHC-P	Assay dependent
IP	Assay dependent
Dot	Assay dependent
EM	Assay dependent

Not tested in other applications.

Calculated MW

59 kDa. ( Note )

Positive Control

rat liver, CHO

Product Note

The antibody recognizes an epitope located on the microtubule-binding peripheral Golgi membrane 58 kDa protein. It is also useful for studies on the effect of microtubule-perturbing agents on the Golgi apparatus.

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

15mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Rat

Immunogen

Golgi 58K protein from rat liver.

Purification

Unpurified

Conjugation

Unconjugated

RRID

AB_370603

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

formimidoyltransferase cyclodeaminase

Cellular Localization

Golgi apparatus

Background

58K protein antibodies are excellent for use as markers for the Golgi complex. The 58K protein has been identified as being FTCD, a bifunctional enzyme that channels 1-carbon units from formiminoglutamate, a metabolite of the histidine degradation pathway, to the folate pool.

Database

Gene ID: 89833 Ftcd
UniProt: O88618 Ftcd

Research Area

REFERENCE

Application Reference

Rolland M et al. J Neurosci 2020; 40 (27) : 5161-5176 Effect of Aβ Oligomers on Neuronal APP Triggers a Vicious Cycle Leading to the Propagation of Synaptic Plasticity Alterations to Healthy Neurons.

Application : ICC/IF

Reactivity : Mouse

Weigand I et al. Sci Rep 2017; 7 (1) : 49 Differential expression of the protein kinase A subunits in normal adrenal glands and adrenocortical adenomas.

Burrack S et al. FASEB J 2012; 26 (8) : 3292-305 A new type of intracellular retention signal identified in a pestivirus structural glycoprotein.

Application : ICC/IF

Reactivity : Pig

Tombolan L et al. Am J Pathol 2011; 179 (5) : 2611-24 High IGFBP2 expression correlates with tumor severity in pediatric rhabdomyosarcoma.

Application : IHC-P

Reactivity : Human

Petrucelli A et al. J Virol 2009; 83 (11) : 5615-29 Characterization of a novel Golgi apparatus-localized latency determinant encoded by human cytomegalovirus.

Application : ICC/IF

Reactivity : Human

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