58K Golgi protein antibody [N1N3]

Name: 58K Golgi protein antibody [N1N3]
Brand: GeneTex
SKU: GTX109972
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-58K Golgi protein antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX109972

Anti-58K Golgi protein antibody [N1N3] used in Western Blot (WB). GTX109972

Cat. No. GTX109972

Reference 1

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-P
Reactivity	Human, Mouse
Package	100 μl ($399), 25 μl ($169)

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View product citations for antibody GTX109972 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

59 kDa. ( Note )

Positive Control

Mouse liver , HepG2

Predict Reactivity

Rat, Bovine, Chicken, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.98 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human 58K Golgi protein. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950316

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

formimidoyltransferase cyclodeaminase , LCHC1

Cellular Localization

Cytoplasm , Golgi apparatus

Background

58K Golgi protein is a bifunctional enzyme that channels 1-carbon units from formiminoglutamate, a metabolite of the histidine degradation pathway, to the folate pool.[supplied by OMIM]

Database

Gene ID: 10841 FTCD
UniProt: O95954 FTCD

Research Area

DATA IMAGES

GTX109972 IHC-P Image

FTCD antibody [N1N3] detects FTCD protein at cytoplasm in human hepatocellular carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human hepatocellular carcinoma.
FTCD antibody [N1N3] (GTX109972) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX109972 WB Image

Sample (50 ug of whole cell lysate)
A: mouse liver
7.5% SDS PAGE
GTX109972 diluted at 1:1000

GTX109972 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
7.5% SDS PAGE
GTX109972 diluted at 1:1000

REFERENCE

Application Reference

Yu Z et al. Cell Signal 2014; 26 (7) : 1560-1566 Using a yeast two-hybrid system to identify FTCD as a new regulator for HIF-1α in HepG2 cells.

Application : WB

Reactivity : Human

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