*Optimal dilutions/concentrations should be determined by the researcher.
Application
Dilution
WB
1:1000-1:10000
ELISA
1:1000-1:10000
Not tested in other applications.
Positive Control
His-recombinant protein , IPTG-induced cell lysate from E. coli expressing His-tagged proteins
PROPERTIES
Form
Liquid
Buffer
PBS
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Immunogen
The immunogen used to generate this antibody corresponds to 6X His tag
Purification
Affinity purified by Protein G.
Conjugation
Unconjugated
RRID
AB_2888083
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
6X His tag antibody , His antibody , 6x His antibody , 6x his tag antibody , His Tag antibody , 6xHis Tag antibody , 6 x His antibody
6X His tag antibody [GT161] detects 6xHis Tag protein by western blot analysis. Lane A and B were IPTG-induced cell lysate from E. coli expressing different GST-tagged proteins. Lane C to H were IPTG induced cell lysate from E. coli expressing different His-tagged protein. 12% SDS-PAGE 6X His tag antibody [GT161] (GTX628921) dilution: 1:5000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX628921 ELISA Image
6X His tag antibody [GT161] detects His-tagged recombinant proteins by indirect ELISA analysis. Various dilutions of His-tagged recombinant proteins coated onto the 96-well plate were detected by using 0.5 μg/ml 6X His tag antibody [GT161] (GTX628914). Bound Abs were further detected by using HRP-conjugated goat anti-mouse IgG (GTX213111-01) with HRP substrates.
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GTX628921 WB Image
6X His tag antibody [GT161] detects 6xHis Tag protein by western blot analysis. A. Non-induced cell lysate from E. coli expressing His-tagged proteins B. IPTG-induced cell lysate from E. coli expressing His-tagged proteins 12% SDS-PAGE 6X His tag antibody [GT161] (GTX628921) dilution: 1:5000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX628921 WB Image
6X His tag antibody [GT161] detects 6xHis Tag protein by western blot analysis. A. 2 μg GST-recombinant protein B. 2 μg His-recombinant protein C. 1 μg His-recombinant protein D. 0.5 μg His-recombinant protein E. 0.1 μg His-recombinant protein 12% SDS-PAGE 6X His tag antibody [GT161] (GTX628921) dilution: 1:5000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.