6X His tag antibody [GT161]

6X His tag antibody [GT161] detects 6xHis Tag protein by western blot analysis.
A. 2 μg GST-recombinant protein
B. 2 μg His-recombinant protein
C. 1 μg His-recombinant protein
D. 0.5 μg His-recombinant protein
E. 0.1 μg His-recombinant protein
12% SDS-PAGE
6X His tag antibody [GT161] (GTX628921) dilution: 1:5000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

6X His tag antibody [GT161] detects His-tagged recombinant proteins by indirect ELISA analysis. Various dilutions of His-tagged recombinant proteins coated onto the 96-well plate were detected by using 0.5 μg/ml 6X His tag antibody [GT161] (GTX628914). Bound Abs were further detected by using HRP-conjugated goat anti-mouse IgG (GTX213111-01) with HRP substrates.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with 6X His tag antibody [GT161] (GTX628921) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

6X His tag antibody [GT161] detects 6xHis Tag protein by western blot analysis.
Lane A and B were IPTG-induced cell lysate from E. coli expressing different GST-tagged proteins.
Lane C to H were IPTG induced cell lysate from E. coli expressing different His-tagged protein.
12% SDS-PAGE
6X His tag antibody [GT161] (GTX628921) dilution: 1:5000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

6X His tag antibody [GT161] detects 6xHis Tag protein by western blot analysis.
A. Non-induced cell lysate from E. coli expressing His-tagged proteins
B. IPTG-induced cell lysate from E. coli expressing His-tagged proteins
12% SDS-PAGE
6X His tag antibody [GT161] (GTX628921) dilution: 1:5000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.