GeneTex
  • Country / Location Selection

United States (US)

6X His tag antibody [GT359] (HRP)

Anti-6X His tag antibody [GT359] (HRP) used in Western Blot (WB). GTX628914-01
Anti-6X His tag antibody [GT359] (HRP) used in Western Blot (WB). GTX628914-01
Anti-6X His tag antibody [GT359] (HRP) used in Western Blot (WB). GTX628914-01
Anti-6X His tag antibody [GT359] (HRP) used in Western Blot (WB). GTX628914-01

Cat No. GTX628914-01

Host

Mouse

Clonality

Monoclonal

Clone Name

GT359

Isotype

IgG3

Application

WB, ELISA
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:20000
ELISA Assay dependent
Not tested in other applications.

Positive Control

IPTG-induced cell lysate from E. coli expressing His-tagged proteins , His-tagged protein1 , His-tagged protein2

PROPERTIES

Form

Liquid

Buffer

0.05M Tris, 0.15M NaCl (pH7.4), 1%BSA. 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.75 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to 6X His tag

Purification

Affinity purified by Protein A.
From tissue culture supernatant

Conjugation

Horseradish peroxidase(HRP)

RRID

AB_2888081

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

6X His tag antibody , His antibody , 6x His antibody , 6x his tag antibody , His Tag antibody , 6xHis Tag antibody , 6 x His antibody

Research Area

DATA IMAGES

Anti-6X His tag antibody [GT359] (HRP) used in Western Blot (WB). GTX628914-01

GTX628914-01 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with 6X His tag antibody [GT359] (GTX628914-01) diluted at 1:5000.

Anti-6X His tag antibody [GT359] (HRP) used in Western Blot (WB). GTX628914-01

GTX628914-01 WB Image

6x His antibody conjugated HRP detects 6x His protein by western blot analysis. IPTG-induced (+, ) cell lysate from E. coli expressing different GST-tagged proteins and His-tagged proteins (2 μl) were separated by 12% SDS-PAGE, and the membrane was blotted with 6x His antibody conjugated HRP (GTX628914-01) at a dilution of 1:20000.

Anti-6X His tag antibody [GT359] (HRP) used in Western Blot (WB). GTX628914-01

GTX628914-01 WB Image

6X His tag antibody [GT359] (HRP) detects 6xHis Tag protein by Western blot analysis.
A. 5 μl non-induced cell lysate from E. coli expressing His-tagged proteins
B. 5 μl IPTG-induced cell lysate from E. coli expressing His-tagged proteins
15 % SDS-PAGE
6X His tag antibody [GT359] (HRP) (GTX628914-01) dilution: 1:5000

Anti-6X His tag antibody [GT359] (HRP) used in Western Blot (WB). GTX628914-01

GTX628914-01 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with 6X His tag antibody [GT359] (GTX628914-01) diluted at 1:5000 and competitor's antibody (sc-8036) diluted at 1:5000.
*The competitor is not affiliated with GeneTex and does not endorse this product.

REFERENCE

REVIEW

There are currently no reviews for 6X His tag antibody [GT359] (HRP) (GTX628914-01). Be the first to share your experience with this product.
SDS
Proclin.pdf
Package List Price ($)
$ 329
$ 169