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6X His tag antibody [GT359]

Anti-6X His tag antibody [GT359] used in Immunoprecipitation (IP). GTX628914
Anti-6X His tag antibody [GT359] used in Western Blot (WB). GTX628914
Anti-6X His tag antibody [GT359] used in Western Blot (WB). GTX628914
Anti-6X His tag antibody [GT359] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628914
Anti-6X His tag antibody [GT359] used in Western Blot (WB). GTX628914
Anti-6X His tag antibody [GT359] used in ELISA (ELISA). GTX628914
Anti-6X His tag antibody [GT359] used in Western Blot (WB). GTX628914
Anti-6X His tag antibody [GT359] used in Western Blot (WB). GTX628914
Anti-6X His tag antibody [GT359] used in Western Blot (WB). GTX628914

Cat No. GTX628914

Host Mouse
Clonality Monoclonal
Clone Name GT359
Isotype IgG3
Application WB, ICC/IF, IP, ELISA
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:2000
IP 1:100-1:500
ELISA 1:1000-1:10000
Not tested in other applications.
PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to 6X His tag

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

6X His tag antibody , His antibody , 6x His antibody , 6x his tag antibody , His Tag antibody , 6xHis Tag antibody , 6 x His antibody

Research Area

DATA IMAGES
Anti-6X His tag antibody [GT359] used in Immunoprecipitation (IP). GTX628914

GTX628914 IP Image

6X His tag antibody [GT359] immunoprecipitates His-fused protein in IP experiments.
IP Sample: cell lysate/extract of His-fused gene transfected HeLa cells
A. 1/20 lysate/extract of transfected HeLa cells
B. Control with 2 μg of preimmune mouse IgG
C. Immunoprecipitation of His-fused protein by 2 μg of 6X His tag antibody [GT359] (GTX628914)
12% SDS-PAGE
The immunoprecipitated His-fused protein was detected by 6X His tag antibody [GT359] (GTX628914) diluted at 1:1000.

Anti-6X His tag antibody [GT359] used in Western Blot (WB). GTX628914

GTX628914 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with 6X His tag antibody [GT359] (GTX628914) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-6X His tag antibody [GT359] used in Western Blot (WB). GTX628914

GTX628914 WB Image

6x His antibody detects 6x His protein by Western blot analysis.
A. Non-induced cell lysates from E. coli expressing HIS-tagged proteins
B. IPTG-induced cell lysates from E. coli expressing HIS-tagged proteins
15 % SDS-PAGE
6x His antibody (GTX628914) dilution: 1:5000

Anti-6X His tag antibody [GT359] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628914

GTX628914 ICC/IF Image

6X His tag antibody [GT359] detects His-recombinant protein by immunofluorescent analysis. Sample: His-tag transfected HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: His-recombinant protein stained by 6X His tag antibody [GT359] (GTX628914) diluted at 1:2000. Blue: Hoechst 33342 staining.

Anti-6X His tag antibody [GT359] used in Western Blot (WB). GTX628914

GTX628914 WB Image

6x His antibody detects 6x His protein by Western blot analysis.
Lane A and B were IPTG-induced cell lysate from E. coli expressing different GST-tagged proteins.
Lane C to G were IPTG induced cell lysate from E. coli expressing different His-tagged protein.
12 % SDS-PAGE
6x His antibody (GTX628914) dilution: 1:5000

Anti-6X His tag antibody [GT359] used in ELISA (ELISA). GTX628914

GTX628914 ELISA Image

6X His tag antibody [GT359] detects His-tagged recombinant proteins by indirect ELISA analysis. Various dilutions of His-tagged recombinant proteins coated onto the 96-well plate were detected by using 0.5 μg/ml 6X His tag antibody [GT359] (GTX628914). Bound Abs were further detected by using HRP-conjugated goat anti-mouse IgG (GTX213111-01) with HRP substrates.

Anti-6X His tag antibody [GT359] used in Western Blot (WB). GTX628914

GTX628914 WB Image

6x His antibody detects 6x His protein by Western blot analysis.
A. 2 μg GST-recombinant protein
B. 2 μg His-recombinant protein
C. 1 μg His-recombinant protein
D. 0.5 μg His-recombinant protein E. 0.1 μg His-recombinant protein
E. 0.1 μg His-recombinant protein E. 0.1 μg His-recombinant protein
12 % SDS-PAGE
6x His antibody (GTX628914) dilution: 1:5000

Anti-6X His tag antibody [GT359] used in Western Blot (WB). GTX628914

GTX628914 WB Image

Interaction of HE4 and recombinant annexin II proteins. A, immunoprecipitation (IP) of annexin II/HE4 complex by anti-HE4 antibody and Western blot analysis with anti-annexin II antibody. Lanes 1and 2, control proteins; lanes 3, 4 and 5, IP by anti-HE4 antibody in ovarian cancer cells OVCAR-3, ES-2, CaoV-3. IB, immunoblotting. B, immunoprecipitation of annexin II/ HE4 complex by anti-annexin II antibody and Western blot analysis with anti- HE4 antibody. Lanes 1 and 2, control proteins; lanes 3, 4 and 5, IP by anti-annexin II antibody in ovarian cancer cells OVCAR-3, ES-2, CaoV-3. Lane6, negative control (Ig G). C, IP of membrane and cytoplasmic proteins by anti-HE4 antibody and Western blot analysis with anti-annexin II antibody. Lanes 1 and 2, membrane proteins of ovarian cancer cells ES-2 and CaoV-3. Lanes 3 and 4, cytoplasmic proteins of ES-2 and CaoV-3. D, IP of membrane and cytoplasmic proteins by anti-annexin II antibody and Western blot analysis with anti-HE4 antibody. Lanes 1 and 2, membrane proteins of ovarian cancer cells ES-2 and CaoV-3. Lanes 3 and 4, cytoplasmic proteins of ES-2 and CaoV-3. Lane 5, negative control (Ig G). E, binding of truncated annexin II mutants, A2-del15 and A2-del26, to GST-HE4. Complexes were immunoprecipitated by anti-HE4 antibody , followed by Western blot analysis with anti-His-tagged antibody. Full-size annexin II was used in lanes 1, A2-del15 was used in lanes 2, and A2-del26 was used in lanes 3. All the plus sign under the panel mean antibodies used.

Anti-6X His tag antibody [GT359] used in Western Blot (WB). GTX628914

GTX628914 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with 6X His tag antibody [GT359] (GTX628914) diluted at 1:1000 and competitor's antibody (sc-8036) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

*The competitor is not affiliated with GeneTex and does not endorse this product.

REFERENCE
REVIEW

6X His tag antibody [GT359] Cat No. GTX628914

Rating ( Average 4 based on 1 users reviews)
Application
Western Blot(WB) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 13-Jun-2014
Score :
Application Tested : WB
Sample : Fission yeast cells transfected His-Tdh1
Amount used : 1μg
Blocking : 3% skim milk in TBS+0.3% tween20, 25°C, 16Hr
Primary Antibody : 1:2000, 25°C, 1Hr
Lane Description : A:Fission yeast cells, B:Fission yeast cells transfected His-Tdh1
Application Reference
SDS
PBS.pdf
Package List Price ($)
$ 319
$ 169