6X His tag antibody [GT359]

6X His tag antibody [GT359] immunoprecipitates His-fused protein in IP experiments.
IP Sample: cell lysate/extract of His-fused gene transfected HeLa cells
A. 1/20 lysate/extract of transfected HeLa cells
B. Control with 2 μg of preimmune mouse IgG
C. Immunoprecipitation of His-fused protein by 2 μg of 6X His tag antibody [GT359] (GTX628914)
12% SDS-PAGE
The immunoprecipitated His-fused protein was detected by 6X His tag antibody [GT359] (GTX628914) diluted at 1:1000.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with 6X His tag antibody [GT359] (GTX628914) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with 6X His tag antibody [GT359] (GTX628914) diluted at 1:1000 and competitor's antibody (sc-8036) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

*The competitor is not affiliated with GeneTex and does not endorse this product.

6X His tag antibody [GT359] detects His-tagged recombinant proteins by indirect ELISA analysis. Various dilutions of His-tagged recombinant proteins coated onto the 96-well plate were detected by using 0.5 μg/ml 6X His tag antibody [GT359] (GTX628914). Bound Abs were further detected by using HRP-conjugated goat anti-mouse IgG (GTX213111-01) with HRP substrates.

6x His antibody detects 6x His protein by Western blot analysis.
Lane A and B were IPTG-induced cell lysate from E. coli expressing different GST-tagged proteins.
Lane C to G were IPTG induced cell lysate from E. coli expressing different His-tagged protein.
12 % SDS-PAGE
6x His antibody (GTX628914) dilution: 1:5000

6x His antibody detects 6x His protein by Western blot analysis.
A. 2 μg GST-recombinant protein
B. 2 μg His-recombinant protein
C. 1 μg His-recombinant protein
D. 0.5 μg His-recombinant protein E. 0.1 μg His-recombinant protein
E. 0.1 μg His-recombinant protein E. 0.1 μg His-recombinant protein
12 % SDS-PAGE
6x His antibody (GTX628914) dilution: 1:5000

6X His tag antibody [GT359] detects 6X His tag protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: 6X His tag stained by 6X His tag antibody [GT359] (GTX628914) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

6x His antibody detects 6x His protein by Western blot analysis.
A. Non-induced cell lysates from E. coli expressing HIS-tagged proteins
B. IPTG-induced cell lysates from E. coli expressing HIS-tagged proteins
15 % SDS-PAGE
6x His antibody (GTX628914) dilution: 1:5000

The data was published in the journal PLoS One in 2014. PMID: 25285446

The data was published in the journal PLoS One in 2014. PMID: 25285446

The data was published in the journal Mol Cancer in 2014. PMID: 25362534

The data was published in the journal J Biomed Sci in 2018.PMID: 29361943