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6X His tag antibody [HIS.H8]

Anti-6X His tag antibody [HIS.H8] used in Western Blot (WB). GTX18184
Anti-6X His tag antibody [HIS.H8] used in Western Blot (WB). GTX18184
Anti-6X His tag antibody [HIS.H8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX18184

Cat. No. GTX18184

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 HIS.H8

Isotype

 IgG2b

Application

 WB, ICC/IF, IP, ELISA

Reactivity

 Species independent
Package
100 μg ($399)
See all 6X His tag products

APPLICATION

Application Note

Recommended Starting Dilutions: For WB: Use at a dilution of 1:1000-1:3000. For ICC/IF: Use at a dilution of 1:500-1:2000. For IP: Use at a dilution of 1:50-1:200. Optimal dilutions should be determined experimentally by the end user.

Product Note

Recognizes the epitope of 6x His-tags encoded by many commercially available vectors, regardless of the tag's location in the fusion protein sequences (i.e.: reacts with N-terminal, C-terminal or internal 6x His-tags)

PROPERTIES

Form

Liquid

Buffer

10mM PBS pH7.2

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Immunogen

HHHHHH (6x His) synthetic peptide conjugated to KLH

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_423119

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

6 His , His tag , 6 His epitope tag , HHHHHH tag , HHHHHH epitope tag

Background

The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.

Research Area

DATA IMAGES

Anti-6X His tag antibody [HIS.H8] used in Western Blot (WB). GTX18184

GTX18184 WB Image

Standard ladder containing five different His-tagged proteins; untransfected control (1), HEK293 cells transfected with His-tagged protein vector (2)

Anti-6X His tag antibody [HIS.H8] used in Western Blot (WB). GTX18184

GTX18184 WB Image

Comparison between anti-His tag (HIS.H8 / EH158) mAb with 2 different vendor Abs, probed against a standard ladder containing five different His-tagged proteins. All Ab dilutions are 1:2000 (0.5μg/mL)
Anti-6X His tag antibody [HIS.H8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX18184

GTX18184 ICC/IF Image

TOP: untransfected control; BOTTOM: anti-His (in red) on His-tagged fusion proteins in HEK293 cells. Both counterstained with DAPI (in blue)

REFERENCE

Application Reference

Christine Kornelia Ott et al. 0 2015; Diverse Aspects of the Sorting and Assembly Machinery in Human Mitochondria
Application : WB
Mabry R et al. MAbs 2010; 2 (1) : 20-34 A dual-targeting PDGFRbeta/VEGF-A molecule assembled from stable antibody fragments demonstrates anti-angiogenic activity in vitro and in vivo.
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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399