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6X His tag antibody [HIS.H8]

Anti-6X His tag antibody [HIS.H8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX18184
Anti-6X His tag antibody [HIS.H8] used in Western Blot (WB). GTX18184
Anti-6X His tag antibody [HIS.H8] used in Western Blot (WB). GTX18184

Cat No. GTX18184

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 HIS.H8

Isotype

 IgG2b

Application

 WB, ICC/IF, IP, ELISA
Package
100 μg ($339)
See all 6X His tag products

APPLICATION

Application Note

Recommended Starting Dilutions: For WB: Use at a dilution of 1:1000-1:3000. For ICC/IF: Use at a dilution of 1:500-1:2000. For IP: Use at a dilution of 1:50-1:200. Optimal dilutions should be determined experimentally by the end user.

Specificity/Sensitivity

Recognizes the epitope of 6x His-tags encoded by many commercially available vectors, regardless of the tag's location in the fusion protein sequences (i.e.: reacts with N-terminal, C-terminal or internal 6x His-tags)

PROPERTIES

Form

Liquid

Buffer

10mM PBS, pH 7.2, 0.05% NaN3

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Immunogen

HHHHHH (6x His) synthetic peptide conjugated to KLH

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_423119

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

6 His , His tag , 6 His epitope tag , HHHHHH tag , HHHHHH epitope tag

Background

The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.

Research Area

DATA IMAGES

Anti-6X His tag antibody [HIS.H8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX18184

GTX18184 ICC/IF Image

TOP: untransfected control; BOTTOM: anti-His (in red) on His-tagged fusion proteins in HEK293 cells. Both counterstained with DAPI (in blue)

Anti-6X His tag antibody [HIS.H8] used in Western Blot (WB). GTX18184

GTX18184 WB Image

Standard ladder containing five different His-tagged proteins; untransfected control (1), HEK293 cells transfected with His-tagged protein vector (2)

Anti-6X His tag antibody [HIS.H8] used in Western Blot (WB). GTX18184

GTX18184 WB Image

Comparison between anti-His tag (HIS.H8 / EH158) mAb with 2 different vendor Abs, probed against a standard ladder containing five different His-tagged proteins. All Ab dilutions are 1:2000 (0.5μg/mL)

REFERENCE

Application Reference

Christine Kornelia Ott et al. 0 2015; Diverse Aspects of the Sorting and Assembly Machinery in Human Mitochondria
Application : WB
Mabry R et al. MAbs 2010; 2 (1) : 20-34 A dual-targeting PDGFRbeta/VEGF-A molecule assembled from stable antibody fragments demonstrates anti-angiogenic activity in vitro and in vivo.
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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 339