Recommended Starting Dilutions:
For WB: Use at a dilution of 1:1000-1:3000.
For ICC/IF: Use at a dilution of 1:500-1:2000.
For IP: Use at a dilution of 1:50-1:200.
Optimal dilutions should be determined experimentally by the end user.
Recognizes the epitope of 6x His-tags encoded by many commercially available vectors, regardless of the tag's location in the fusion protein sequences (i.e.: reacts with N-terminal, C-terminal or internal 6x His-tags)
10mM PBS pH7.2
0.05% Sodium azide
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Batch dependent (Please refer to the vial label for the specific concentration.)
HHHHHH (6x His) synthetic peptide conjugated to KLH
Protein A purified
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
6 His , His tag , 6 His epitope tag , HHHHHH tag , HHHHHH epitope tag
The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.