Recommended Starting Dilutions:
For WB: Use at a dilution of 1:1000-1:3000.
For ICC/IF: Use at a dilution of 1:500-1:2000.
For IP: Use at a dilution of 1:50-1:200.
Optimal dilutions should be determined experimentally by the end user.
Recognizes the epitope of 6x His-tags encoded by many commercially available vectors, regardless of the tag's location in the fusion protein sequences (i.e.: reacts with N-terminal, C-terminal or internal 6x His-tags)
10mM PBS, pH 7.2, 0.05% NaN3
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Batch dependent (Please refer to the vial label for the specific concentration.)
HHHHHH (6x His) synthetic peptide conjugated to KLH
Protein A purified
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
6 His , His tag , 6 His epitope tag , HHHHHH tag , HHHHHH epitope tag
The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.