GeneTex
United States (US)

6X His tag antibody (HRP)

GTX115045-01_40435_WB_18121410_605.jpg

Cat No. GTX115045-01

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Positive Control

E.coli Lysate harbouring His-tagged recombinant protein
PROPERTIES

Form

Liquid

Buffer

TBS, pH 7.4 containing 10mg/ml BSA, 0.005% thimerosal as a preservative.

Concentration

0.779mg/ml

Immunogen

Rabbits were immunized with HHHHHH conjugated with KLH. After multiple immunizations in Freunds adjuvant serum was collected. Antibodies were immunoaffinity purified using the peptide immobilized on a solid support

Conjugation

Horseradish peroxidase(HRP)

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

6X His tag antibody, His antibody, 6x His antibody, 6x his tag antibody, His Tag antibody, 6xHis Tag antibody, 6 x His antibody
DATA IMAGES
GTX115045-01_40435_WB_18121410_605.jpg

GTX115045-01 WB Image

Western blot analysis of extracts from untransfected control cells and transfected cells overexpressing His-tagged protein (~27 kDa), using 6X 6X His tag antibody conjugated with HRP.

A: 10ug E.coli Lysate harbouring His-tagged
recombinant protein (N-terminal linked),
non-induced (10 ug)

B: 10ug E.coli Lysate harbouring His-tagged
recombinant protein (N-terminal linked),
induced (10 ug)

12% SDS PAGE

GTX115045-01 diluted at 1:500

Package List Price ($)
$ 319
$ 169