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6X His tag antibody (HRP)

Anti-6X His tag antibody (HRP) used in Western Blot (WB). GTX30506

Cat No. GTX30506

Reference 1

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, ELISA, IHC
Package
100 μg ($369)
See all 6X His tag products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1,000-1:5,000
ICC/IF 1:200-1:500
ELISA 1:10,000-1:100,000
IHC Assay dependent
Not tested in other applications.

Product Note

This antibody does not recognize all N-terminal 6-His tagged proteins.

PROPERTIES

Form

Liquid

Buffer

PBS pH 7.2, 0.2% BSA, 0.1% Pro-Clean 400

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

6-His (HHHHHH) conjugated to KLH.

Purification

Purified by antigen-affinity chromatography

Conjugation

Horseradish peroxidase(HRP)
Ratio : 4 HRP molecules per IgG molecule.

RRID

AB_385694

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Research Area

DATA IMAGES

Anti-6X His tag antibody (HRP) used in Western Blot (WB). GTX30506

GTX30506 WB Image

WB analysis of 200, 100, and 50ng of E. coli lysate containing tagged fusion protein using GTX30506 6X His tag antibody (HRP).
Dilution : 1:5000

REFERENCE

Application Reference

Ng WK et al. Protein Expr Purif 2016; 127 : 73-80 Expression of soluble human Neonatal Fc-receptor (FcRn) in Escherichia coli through modification of growth environment.
Application : WB
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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 369