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6X His tag antibody [RM146]

Anti-6X His tag antibody [RM146] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33607
Anti-6X His tag antibody [RM146] used in Western Blot (WB). GTX33607
Anti-6X His tag antibody [RM146] used in Immunoprecipitation (IP). GTX33607

Cat No. GTX33607

Host

Rabbit

Clonality

Monoclonal

Clone Name

RM146

Isotype

IgG

Application

WB, ICC/IF, FACS, IP, IHC
Package
100 μg ($349)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.1 μg/mL - 1 μg/mL
ICC/IF 0.5 μg/mL - 2 μg/mL
FACS 0.5 μg/mL - 2 μg/mL
IP 0.5 μg/mL - 2 μg/mL
IHC 0.1 μg/mL - 1 μg/mL
Not tested in other applications.

Specificity/Sensitivity

This antibody reacts to recombinant proteins containing the 6xHis-Tag or 10xHis-Tag fused to either the amino or carboxy terminus. No cross reactivity with other endogenous protein in mammalian or bacteria cells.

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 0.09% sodium azide, 50% Glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Immunogen

Mixture of a peptide with 6xHis-Tag at the N-terminus and another peptide with 6xHis-Tag at the C-terminus.

Purification

Protein A purified
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

DATA IMAGES

Anti-6X His tag antibody [RM146] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33607

GTX33607 ICC/IF Image

ICC/IF analysis of 293T cells expressing His-Tag nuclear protein X using GTX33607 6X His tag antibody [RM146].

Anti-6X His tag antibody [RM146] used in Western Blot (WB). GTX33607

GTX33607 WB Image

WB analysis of various sample lysayes using GTX33607 6X His tag antibody [RM146].
Lane 1 : 293T cells
Lane 2 : 293T cells transfected with His-Tag fusion protein X
Lane 3 : E. coli lysate without His-Tag Protein Y
Lane 4 : E. coli lysate with His-Tag Protein Y
Dilution : 0.2μg/ml

Anti-6X His tag antibody [RM146] used in Immunoprecipitation (IP). GTX33607

GTX33607 IP Image

IP analysis of 293T cells expressing a His-Tag fusion protein using GTX33607 6X His tag antibody [RM146].
Lane 1 : Whole lysate control
Lane 2 : IP by rabbit IgG control
Lane 3 : IP by Primary antibody

REFERENCE

There are currently no references for 6X His tag antibody [RM146] (GTX33607). Be the first to share your publications with this product.

REVIEW

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Package List Price ($)
$ 349