6X His tag antibody [RM146]

Cat. No. GTX33607

Host	Rabbit
Clonality	Monoclonal
Clone Name	RM146
Isotype	IgG
Application	WB, ICC/IF, FACS, IP, IHC
Reactivity	Species independent
Package	100 μg ($409)

See all 6X His tag products

Datasheet

View product citations for antibody GTX33607 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	0.1 μg/mL - 1 μg/mL
ICC/IF	0.5 μg/mL - 2 μg/mL
FACS	0.5 μg/mL - 2 μg/mL
IP	0.5 μg/mL - 2 μg/mL
IHC	0.1 μg/mL - 1 μg/mL

Not tested in other applications.

Product Note

This antibody reacts to recombinant proteins containing the 6xHis-Tag or 10xHis-Tag fused to either the amino or carboxy terminus. No cross reactivity with other endogenous protein in mammalian or bacteria cells.

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Immunogen

Mixture of a peptide with 6xHis-Tag at the N-terminus and another peptide with 6xHis-Tag at the C-terminus.

Purification

Protein A purified
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

GTX33607 ICC/IF Image

ICC/IF analysis of 293T cells expressing His-Tag nuclear protein X using GTX33607 6X His tag antibody [RM146].

GTX33607 WB Image

WB analysis of various sample lysayes using GTX33607 6X His tag antibody [RM146].
Lane 1 : 293T cells
Lane 2 : 293T cells transfected with His-Tag fusion protein X
Lane 3 : E. coli lysate without His-Tag Protein Y
Lane 4 : E. coli lysate with His-Tag Protein Y
Dilution : 0.2μg/ml

GTX33607 IP Image

IP analysis of 293T cells expressing a His-Tag fusion protein using GTX33607 6X His tag antibody [RM146].
Lane 1 : Whole lysate control
Lane 2 : IP by rabbit IgG control
Lane 3 : IP by Primary antibody

REFERENCE

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REVIEW

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Package

List Price ($)

100 μg

$ 409

Qty

6X His tag antibody [RM146]

Cat. No. GTX33607

Host

Clonality

Clone Name

Isotype

Application

Reactivity