GeneTex
United States (US)

6X His tag antibody [RM146]

GTX33607_20160517_IP_18121410_709.jpg
GTX33607_20160517_IHC_18121410_221.jpg
GTX33607_20160517_IHC_1_18121410_361.jpg
GTX33607_20160517_WB_18121410_507.jpg

Cat No. GTX33607

Host Rabbit
Clonality Monoclonal
Clone Name RM146
Isotype IgG
Application WB, ICC/IF, FACS, IP, IHC
APPLICATION

Application Note

Western Blot: 0.1 ug/mL – 1 ug/mL, Immunoprecipitation: 0.5 ug/mL – 2 ug/mL, Immunocytochemistry 0.5 ug/mL – 2 ug/mL, Flow Cytometry: 0.5 ug/mL – 2 ug/mL, Immunohistochemistry 0.1 ug/mL-1 ug/mL.

Specificity/Sensitivity

This antibody reacts to recombinant proteins containing the 6xHis-Tag or 10xHis-Tag fused to either the amino or carboxy terminus. No cross reactivity with other endogenous protein in mammalian or bacteria cells.
PROPERTIES

Form

Liquid

Buffer

50% Glycerol/PBS with 1% BSA and 0.09% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Mixture of a peptide with 6xHis-Tag at the N-terminus and another peptide with 6xHis-Tag at the C-terminus.

Purification

Protein A affinity purified from an animal origin-free culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Research Area

DATA IMAGES
GTX33607_20160517_IP_18121410_709.jpg

GTX33607 IP Image

Immunoprecipitation of 293T cells expressing a His-Tag fusion protein by using anti- His tag clone RM146, was blotted with RM146. (1) Whole lysate control; (2) IP by rabbit IgG control; (3) IP by RM146.

GTX33607_20160517_IHC_18121410_221.jpg

GTX33607 IHC Image

Immunohistochemistry staining of naive HepG2 cells(Negative control) using anti-His-Tag antibody, RM146.

GTX33607_20160517_IHC_1_18121410_361.jpg

GTX33607 IHC Image

Immunohistochemistry staining of 293T cells expressing His-Tag nuclear protein X, using anti-His-Tag antibody, RM146.

GTX33607_20160517_WB_18121410_507.jpg

GTX33607 WB Image

Western blot of 293T cells: (1) untransfected or (2) transfected with His-Tag fusion protein X and (3) E. coli lysate without His-Tag Protein Y (4) E. coli lysate with His-Tag Protein Y using anti- His tag Clone RM146 at 0.2 ug/mL.

Package List Price ($)
$ 319