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6X His tag antibody

Anti-6X His tag antibody used in Immunoprecipitation (IP). GTX115045
Anti-6X His tag antibody used in Western Blot (WB). GTX115045
Anti-6X His tag antibody used in ELISA (ELISA). GTX115045
Anti-6X His tag antibody used in Western Blot (WB). GTX115045

Cat No. GTX115045

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-Wm, IP, ELISA
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
IHC-Wm Assay dependent
IP 1:100-1:500
ELISA 1:1000-1:10000
Not tested in other applications.

Positive Control

His-tagged recombinant protein

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to 6X His tag

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036130

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

6X His tag antibody , His antibody , 6x His antibody , 6x his tag antibody , His Tag antibody , 6xHis Tag antibody , 6 x His antibody

Research Area

DATA IMAGES

Anti-6X His tag antibody used in Immunoprecipitation (IP). GTX115045

GTX115045 IP Image

His-tagged protein was immunoprecipitated from transfected cells using 8 ug of 6X His tag antibody (GTX115045). The precipitated His-tagged protein was detected by GTX115045 diluted at 1:500, and used EasyBlot anti-rabbit IgG (GTX221666-01) as secondary reagent.

Anti-6X His tag antibody used in Western Blot (WB). GTX115045

GTX115045 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with 6X His tag antibody (GTX115045) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-6X His tag antibody used in ELISA (ELISA). GTX115045

GTX115045 ELISA Image

6X His tag antibody detects His-tagged recombinant proteins by indirect ELISA analysis. Various dilutions of His-tagged recombinant proteins coated onto the 96-well plate were detected by using 2.5 μg/ml 6x His antibody (GTX115045). Bound Abs were further detected by using HRP-conjugated goat anti-rabbit IgG (GTX213110-01) with HRP substrates.

Anti-6X His tag antibody used in Western Blot (WB). GTX115045

GTX115045 WB Image

Sample
A: 10 μg of non-transformed E. coli lysate
B: 10 μg of non-transformed E. coli lysate, induced
C: 10ug E.coli Lysate harbouring His-tagged recombinant protein (N-terminal linked), non-induced
D: 10ug E.coli Lysate harbouring His-tagged recombinant protein (N-terminal linked), induced
12% SDS PAGE
GTX115045 diluated at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

6X His tag antibody Cat No. GTX115045

Rating ( Average 3 based on 1 users reviews)
Application
IHC (Whole mount)(IHC-Wm) ( Average 3 based on 1 users reviews)
Date : Anonymous submitted on 27-Mar-2015
Score :
Application Tested : IHC-Wm
Sample : 16 hpf zebrafish embryo tissue
Blocking : 10% sheep serum, 0.8 % Triton X-100, 1 % BSA in PBST, 4°C, 3Hr
Fixation : 4% PFA in PBS
Permeabilization /Antigen retrieval : 150 mM Tris-Cl (pH 9.0), 70°C, 15minHr
Primary Antibody : 1:200, 4°C, 16Hr
Figure Note : His-zebrafish stmn4 protein overexpressed in zebrafish embryo. Red:His-stmn4 protein stained by 6x his tag antibody(GTX115045). Green:Tubulin. Blue:DAPI staining.
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169