GeneTex
United States (US)

6X His tag antibody

GTX115045_40149_IP_18121410_103.jpg
GTX115045_40863_20180921_WB_B_18121410_379.jpg
GTX115045_40863_ELISA_18121410_112.jpg
GTX115045_WB_18121410_185.jpg
GTX115045_40863_WB_18121410_851.jpg

Cat No. GTX115045

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-Wm, IP, ELISA
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
IHC-Wm Assay dependent
IP 1:100-1:500
ELISA 1:1000-1:10000
Not tested in other applications.

Positive Control

His-tagged recombinant protein
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to 6X His tag

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

6X His tag antibody, His antibody, 6x His antibody, 6x his tag antibody, His Tag antibody, 6xHis Tag antibody, 6 x His antibody

Research Area

DATA IMAGES
GTX115045_40149_IP_18121410_103.jpg

GTX115045 IP Image

His-tagged protein was immunoprecipitated from transfected cells using 8 ug of 6X His tag antibody (GTX115045). The precipitated His-tagged protein was detected by GTX115045 diluted at 1:500, and used EasyBlot anti-rabbit IgG (GTX221666-01) as secondary reagent.

GTX115045_40863_20180921_WB_B_18121410_379.jpg

GTX115045 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with 6X His tag antibody (GTX115045) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX115045_40863_ELISA_18121410_112.jpg

GTX115045 ELISA Image

6X His tag antibody detects His-tagged recombinant proteins by indirect ELISA analysis. Various dilutions of His-tagged recombinant proteins coated onto the 96-well plate were detected by using 2.5 μg/ml 6x His antibody (GTX115045). Bound Abs were further detected by using HRP-conjugated goat anti-rabbit IgG (GTX213110-01) with HRP substrates.

GTX115045_WB_18121410_185.jpg

GTX115045 WB Image

Sample
A: 10 µg of non-transformed E. coli lysate
B: 10 µg of non-transformed E. coli lysate, induced
C: 10ug E.coli Lysate harbouring His-tagged recombinant protein (N-terminal linked), non-induced
D: 10ug E.coli Lysate harbouring His-tagged recombinant protein (N-terminal linked), induced
12% SDS PAGE
GTX115045 diluated at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX115045_40863_WB_18121410_851.jpg

GTX115045 WB Image

Western blot analysis of extracts from untransfected control cells and transfected cells overexpressing His-tagged protein (~19 kDa), using 6X His tag antibody. A: 10ug E.coli Lysate harbouring His-tagged recombinant protein (N-terminal linked), non-induced (10 µg). B: 10ug E.coli Lysate harbouring His-tagged recombinant protein (N-terminal linked), induced (10 µg). The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW

6X His tag antibody Cat No. GTX115045

Rating ( Average 3 based on 1 users reviews)
Application
IHC (Whole mount)(IHC-Wm) ( Average 3 based on 1 users reviews)
Date : Anonymous submitted on 27-Mar-2015
Score :
Application Tested : IHC-Wm
Sample : 16 hpf zebrafish embryo tissue
Blocking : 10% sheep serum, 0.8 % Triton X-100, 1 % BSA in PBST, 4°C, 3Hr
Fixation : 4% PFA in PBS
Permeabilization /Antigen retrieval : 150 mM Tris-Cl (pH 9.0), 70°C, 15minHr
Primary Antibody : 1:200, 4°C, 16Hr
Figure Note : His-zebrafish stmn4 protein overexpressed in zebrafish embryo. Red:His-stmn4 protein stained by 6x his tag antibody(GTX115045). Green:Tubulin. Blue:DAPI staining.
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169