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6X His tag antibody

Anti-6X His tag antibody used in Immunoprecipitation (IP). GTX115045
Anti-6X His tag antibody used in Western Blot (WB). GTX115045
Anti-6X His tag antibody used in ELISA (ELISA). GTX115045
Anti-6X His tag antibody used in Western Blot (WB). GTX115045

Cat No. GTX115045

Reference 11
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-Wm, IP, ELISA
Package
100 μl ($329),
25 μl ($169)
See all 6X His tag products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
IHC-Wm Assay dependent
IP 1:100-1:500
ELISA 1:1000-1:10000
Not tested in other applications.

Positive Control

His-tagged recombinant protein

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to 6X His tag

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036130

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

6X His tag antibody , His antibody , 6x His antibody , 6x his tag antibody , His Tag antibody , 6xHis Tag antibody , 6 x His antibody

Research Area

DATA IMAGES

Anti-6X His tag antibody used in Immunoprecipitation (IP). GTX115045

GTX115045 IP Image

His-tagged protein was immunoprecipitated from transfected cells using 8 ug of 6X His tag antibody (GTX115045). The precipitated His-tagged protein was detected by GTX115045 diluted at 1:500, and used EasyBlot anti-rabbit IgG (GTX221666-01) as secondary reagent.

Anti-6X His tag antibody used in Western Blot (WB). GTX115045

GTX115045 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with 6X His tag antibody (GTX115045) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-6X His tag antibody used in ELISA (ELISA). GTX115045

GTX115045 ELISA Image

6X His tag antibody detects His-tagged recombinant proteins by indirect ELISA analysis. Various dilutions of His-tagged recombinant proteins coated onto the 96-well plate were detected by using 2.5 μg/ml 6x His antibody (GTX115045). Bound Abs were further detected by using HRP-conjugated goat anti-rabbit IgG (GTX213110-01) with HRP substrates.

Anti-6X His tag antibody used in Western Blot (WB). GTX115045

GTX115045 WB Image

Sample
A: 10 μg of non-transformed E. coli lysate
B: 10 μg of non-transformed E. coli lysate, induced
C: 10ug E.coli Lysate harbouring His-tagged recombinant protein (N-terminal linked), non-induced
D: 10ug E.coli Lysate harbouring His-tagged recombinant protein (N-terminal linked), induced
12% SDS PAGE
GTX115045 diluated at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Canela-P?rez I et al. Parasitol Res 2020; 119 (11) : 3899-3907 Trypanosoma cruzi Importin α: ability to bind to a functional classical nuclear localization signal of the bipartite type.
Ewelina ?ojewska et al. Plant Biotechnology Reports 2019; (Epub) Production of recombinant colicin M in Nicotiana tabacum plants and its antimicrobial activity.
Application : WB
Jiang MC et al. Viruses 2019; 11 (6) Production of Human IFN帠 Protein in Nicotiana benthamiana Plant through an Enhanced Expression System Based on Bamboo mosaic Virus.
Liu W et al. Microbiologyopen 2018; (Epub) Sensor kinase KinB and its pathway-associated key factors sense the signal of nutrition starvation in sporulation of Bacillus-subtilis.
Application : WB
Chang CC et al. Sci Rep 2018; 8 (1) : 9786 The role of sentrin-specific protease 2 substrate recognition in TGF-β-induced tumorigenesis.
Application : WB
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REVIEW

6X His tag antibody Cat No. GTX115045

Rating
( Average 3 based on 1 users reviews)
Application
IHC (Whole mount)(IHC-Wm)
( Average 3 based on 1 users reviews)
Anti-6X His tag antibody used in IHC (Whole mount) (IHC-Wm). GTX115045
Date : Anonymous submitted on 27-Mar-2015
Score :
Application Tested : IHC-Wm
Sample : 16 hpf zebrafish embryo tissue
Blocking : 10% sheep serum, 0.8 % Triton X-100, 1 % BSA in PBST, 4°C, 3Hr
Fixation : 4% PFA in PBS
Permeabilization /Antigen retrieval : 150 mM Tris-Cl (pH 9.0), 70°C, 15minHr
Primary Antibody : 1:200, 4°C, 16Hr
Figure Note : His-zebrafish stmn4 protein overexpressed in zebrafish embryo. Red:His-stmn4 protein stained by 6x his tag antibody(GTX115045). Green:Tubulin. Blue:DAPI staining.
Submit a Review
Application Reference
Canela-P?rez I et al. Parasitol Res 2020; 119 (11):3899-3907 Trypanosoma cruzi Importin α: ability to bind to a functional classical nuclear localization signal of the bipartite type.
Ewelina ?ojewska et al. Plant Biotechnology Reports 2019; (Epub) Production of recombinant colicin M in Nicotiana tabacum plants and its antimicrobial activity.
Application : WB
Jiang MC et al. Viruses 2019; 11 (6) Production of Human IFN帠 Protein in Nicotiana benthamiana Plant through an Enhanced Expression System Based on Bamboo mosaic Virus.
Liu W et al. Microbiologyopen 2018; (Epub) Sensor kinase KinB and its pathway-associated key factors sense the signal of nutrition starvation in sporulation of Bacillus-subtilis.
Application : WB
Chang CC et al. Sci Rep 2018; 8 (1):9786 The role of sentrin-specific protease 2 substrate recognition in TGF-β-induced tumorigenesis.
Application : WB
Pohlers S et al. MBio 2017; 8 (1) Lipid Signaling via Pkh1/2 Regulates Fungal CO2 Sensing through the Kinase Sch9.
Lin MJ et al. Sci Rep 2016; 6:36188 Stathmin-like 4 is critical for the maintenance of neural progenitor cells in dorsal midbrain of zebrafish larvae.
Application : IHC-Wm
Yamaoka Y et al. Front Microbiol 2016; 7:509 Development of Monoclonal Antibody and Diagnostic Test for Middle East Respiratory Syndrome Coronavirus Using Cell-Free Synthesized Nucleocapsid Antigen.
Application : WB
Huang SW et al. Virus Res. 2015; (Epub) Dual ATPase and GTPase activity of the replication-associated protein (Rep) of beak and feather disease virus.
Application : WB
Tseng YY et al. J Virol 2015; 89 (9):4966-79 Functional analysis of the short isoform of orf virus protein OV20.0.
Application : WB
Parham C et al. J Alzheimers Dis 2014; 38 (2):415-23 Perlecan domain V inhibits amyloid-β induced brain endothelial cell toxicity and restores angiogenic function.
Application : WB
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169