6X His tag antibody

Cat No. GTX115045

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-Wm, IP, ELISA

See all 6X His tag products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:5000-1:20000
IHC-Wm	Assay dependent
IP	1:100-1:500
ELISA	1:1000-1:10000

Not tested in other applications.

Positive Control

His-tagged recombinant protein

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to 6X His tag

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

6X His tag antibody, His antibody, 6x His antibody, 6x his tag antibody, His Tag antibody, 6xHis Tag antibody, 6 x His antibody

Research Area

- Epitope Tags & Reporters

DATA IMAGES

GTX115045 IP Image

His-tagged protein was immunoprecipitated from transfected cells using 8 ug of 6X His tag antibody (GTX115045). The precipitated His-tagged protein was detected by GTX115045 diluted at 1:500, and used EasyBlot anti-rabbit IgG (GTX221666-01) as secondary reagent.

GTX115045 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with 6X His tag antibody (GTX115045) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX115045 ELISA Image

6X His tag antibody detects His-tagged recombinant proteins by indirect ELISA analysis. Various dilutions of His-tagged recombinant proteins coated onto the 96-well plate were detected by using 2.5 μg/ml 6x His antibody (GTX115045). Bound Abs were further detected by using HRP-conjugated goat anti-rabbit IgG (GTX213110-01) with HRP substrates.

GTX115045 WB Image

Sample
A: 10 µg of non-transformed E. coli lysate
B: 10 µg of non-transformed E. coli lysate, induced
C: 10ug E.coli Lysate harbouring His-tagged recombinant protein (N-terminal linked), non-induced
D: 10ug E.coli Lysate harbouring His-tagged recombinant protein (N-terminal linked), induced
12% SDS PAGE
GTX115045 diluated at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX115045 WB Image

Western blot analysis of extracts from untransfected control cells and transfected cells overexpressing His-tagged protein (~19 kDa), using 6X His tag antibody. A: 10ug E.coli Lysate harbouring His-tagged recombinant protein (N-terminal linked), non-induced (10 µg). B: 10ug E.coli Lysate harbouring His-tagged recombinant protein (N-terminal linked), induced (10 µg). The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Liu W et al. Microbiologyopen 2018; (Epub ahead of print) Sensor kinase KinB and its pathway-associated key factors sense the signal of nutrition starvation in sporulation of Bacillus-subtilis.

Application : WB

Chang CC et al. Sci Rep 2018; 8 (1) : 9786 The role of sentrin-specific protease 2 substrate recognition in TGF-β-induced tumorigenesis.

Application : WB

Pohlers S et al. MBio 2017; 8 (1) Lipid Signaling via Pkh1/2 Regulates Fungal CO2 Sensing through the Kinase Sch9.

Lin MJ et al. Sci Rep 2016; 6 : 36188 Stathmin-like 4 is critical for the maintenance of neural progenitor cells in dorsal midbrain of zebrafish larvae.

Application : IHC-Wm

Yamaoka Y et al. Front Microbiol 2016; 7 : 509 Development of Monoclonal Antibody and Diagnostic Test for Middle East Respiratory Syndrome Coronavirus Using Cell-Free Synthesized Nucleocapsid Antigen.

Application : WB

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