GeneTex

  • Country / Location Selection

United States (US)

6X His tag antibody

Anti-6X His tag antibody used in Immunoprecipitation (IP). GTX115045
Anti-6X His tag antibody used in ELISA (ELISA). GTX115045
Anti-6X His tag antibody used in Western Blot (WB). GTX115045
Anti-6X His tag antibody used in Western Blot (WB). GTX115045

Cat. No. GTX115045

Reference 16
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-Wm, IP, ELISA

Reactivity

 Species independent
Package
100 μl ($399),
25 μl ($169)
See all 6X His tag products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
IHC-Wm Assay dependent
IP 1:100-1:500
ELISA 1:1000-1:10000
Not tested in other applications.

Positive Control

His-tagged recombinant protein

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to 6X His tag

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036130

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

6X His tag antibody , His antibody , 6x His antibody , 6x his tag antibody , His Tag antibody , 6xHis Tag antibody , 6 x His antibody

Research Area

DATA IMAGES

Anti-6X His tag antibody used in Immunoprecipitation (IP). GTX115045

GTX115045 IP Image

His-tagged protein was immunoprecipitated from transfected cells using 8 ug of 6X His tag antibody (GTX115045). The precipitated His-tagged protein was detected by GTX115045 diluted at 1:500, and used EasyBlot anti-rabbit IgG (GTX221666-01) as secondary reagent.

Anti-6X His tag antibody used in ELISA (ELISA). GTX115045

GTX115045 ELISA Image

6X His tag antibody detects His-tagged recombinant proteins by indirect ELISA analysis. Various dilutions of His-tagged recombinant proteins coated onto the 96-well plate were detected by using 2.5 μg/ml 6x His antibody (GTX115045). Bound Abs were further detected by using HRP-conjugated goat anti-rabbit IgG (GTX213110-01) with HRP substrates.

Anti-6X His tag antibody used in Western Blot (WB). GTX115045

GTX115045 WB Image

Sample
A: 10 μg of non-transformed E. coli lysate
B: 10 μg of non-transformed E. coli lysate, induced
C: 10ug E.coli Lysate harbouring His-tagged recombinant protein (N-terminal linked), non-induced
D: 10ug E.coli Lysate harbouring His-tagged recombinant protein (N-terminal linked), induced
12% SDS PAGE
GTX115045 diluated at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-6X His tag antibody used in Western Blot (WB). GTX115045

GTX115045 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with 6X His tag antibody (GTX115045) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

WC Chiou et al. Biomed Pharmacother 2023; 170 : 116077 Ergosterol peroxide blocks HDV infection as a novel entry inhibitor by targeting human NTCP receptor
Ebert S et al. Cells 2023; 12 (14) Tissue Inhibitor of Metalloproteinases-1 Interacts with CD74 to Promote AKT Signaling, Monocyte Recruitment Responses, and Vascular Smooth Muscle Cell Proliferation.
Gonz?lez-Gonz?lez L et al. Tissue Barriers 2022; 10 (2) : 1994351 ZO-2 favors Hippo signaling, and its re-expression in the steatotic liver by AMPK restores junctional sealing.
Han-Hsiang Chen et al. J Biol Chem 2022; 298 (6) : 101957 Development of antiviral carbon quantum dots that target the Japanese encephalitis virus envelope protein.
Christian Hern?ndez-Guzm?n et al. Cells 2021; 10 (10) Zonula occludens 2 and Cell-Cell Contacts Are Required for Normal Nuclear Shape in Epithelia.
Application : WB
Show More +
Submit a Reference

REVIEW

6X His tag antibody Cat. No. GTX115045

Rating
( Average 3 based on 1 users reviews)
Application
IHC (Whole mount)(IHC-Wm)
( Average 3 based on 1 users reviews)
Anti-6X His tag antibody used in IHC (Whole mount) (IHC-Wm). GTX115045
Date : Anonymous submitted on 27-Mar-2015
Score :
Application Tested : IHC-Wm
Sample : 16 hpf zebrafish embryo tissue
Blocking : 10% sheep serum, 0.8 % Triton X-100, 1 % BSA in PBST, 4°C, 3Hr
Fixation : 4% PFA in PBS
Permeabilization /Antigen retrieval : 150 mM Tris-Cl (pH 9.0), 70°C, 15minHr
Primary Antibody : 1:200, 4°C, 16Hr
Figure Note : His-zebrafish stmn4 protein overexpressed in zebrafish embryo. Red:His-stmn4 protein stained by 6x his tag antibody(GTX115045). Green:Tubulin. Blue:DAPI staining.
Submit a Review
Application Reference
WC Chiou et al. Biomed Pharmacother 2023; 170:116077 Ergosterol peroxide blocks HDV infection as a novel entry inhibitor by targeting human NTCP receptor
Ebert S et al. Cells 2023; 12 (14) Tissue Inhibitor of Metalloproteinases-1 Interacts with CD74 to Promote AKT Signaling, Monocyte Recruitment Responses, and Vascular Smooth Muscle Cell Proliferation.
Gonz?lez-Gonz?lez L et al. Tissue Barriers 2022; 10 (2):1994351 ZO-2 favors Hippo signaling, and its re-expression in the steatotic liver by AMPK restores junctional sealing.
Han-Hsiang Chen et al. J Biol Chem 2022; 298 (6):101957 Development of antiviral carbon quantum dots that target the Japanese encephalitis virus envelope protein.
Christian Hern?ndez-Guzm?n et al. Cells 2021; 10 (10) Zonula occludens 2 and Cell-Cell Contacts Are Required for Normal Nuclear Shape in Epithelia.
Application : WB
Canela-P?rez I et al. Parasitol Res 2020; 119 (11):3899-3907 Trypanosoma cruzi Importin α: ability to bind to a functional classical nuclear localization signal of the bipartite type.
Ewelina ?ojewska et al. Plant Biotechnology Reports 2019; (Epub) Production of recombinant colicin M in Nicotiana tabacum plants and its antimicrobial activity.
Application : WB
Jiang MC et al. Viruses 2019; 11 (6) Production of Human IFN帠 Protein in Nicotiana benthamiana Plant through an Enhanced Expression System Based on Bamboo mosaic Virus.
Liu W et al. Microbiologyopen 2018; (Epub) Sensor kinase KinB and its pathway-associated key factors sense the signal of nutrition starvation in sporulation of Bacillus-subtilis.
Application : WB
Chang CC et al. Sci Rep 2018; 8 (1):9786 The role of sentrin-specific protease 2 substrate recognition in TGF-β-induced tumorigenesis.
Application : WB
Pohlers S et al. MBio 2017; 8 (1) Lipid Signaling via Pkh1/2 Regulates Fungal CO2 Sensing through the Kinase Sch9.
Lin MJ et al. Sci Rep 2016; 6:36188 Stathmin-like 4 is critical for the maintenance of neural progenitor cells in dorsal midbrain of zebrafish larvae.
Application : IHC-Wm
Yamaoka Y et al. Front Microbiol 2016; 7:509 Development of Monoclonal Antibody and Diagnostic Test for Middle East Respiratory Syndrome Coronavirus Using Cell-Free Synthesized Nucleocapsid Antigen.
Application : WB
Huang SW et al. Virus Res. 2015; (Epub) Dual ATPase and GTPase activity of the replication-associated protein (Rep) of beak and feather disease virus.
Application : WB
Tseng YY et al. J Virol 2015; 89 (9):4966-79 Functional analysis of the short isoform of orf virus protein OV20.0.
Application : WB
Parham C et al. J Alzheimers Dis 2014; 38 (2):415-23 Perlecan domain V inhibits amyloid-β induced brain endothelial cell toxicity and restores angiogenic function.
Application : WB
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169