6X His tag antibody
Host
|
Goat
|
Clonality
|
Polyclonal
|
Isotype
|
IgG
|
Application
|
WB, ICC/IF, IP, ELISA, IHC
|
Package
|
100 μl ($399)
|
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:100- 1:500 |
1:100-1:400 |
1-4μg antibody/mg lysate |
1:1,000-1:30,000 (Detection) ; 1:100-1:500 (Coating) |
Assay dependent |
Not tested in other applications.
Product Note
This antibody does not recognize all N-terminal 6-His tagged proteins.
PROPERTIES
Form
Liquid
Buffer
PBS pH7.2
Preservative
0.09% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Immunogen
6-His (HHHHHH) conjugated to KLH.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
RRID
AB_385678
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
DATA IMAGES
|
GTX30500 WB Image
WB analysis of 200, 100, and 50ng of E. coli lysate containing tagged fusion protein using GTX30500 6X His tag antibody. Dilution : 1:5000
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REFERENCE
REVIEW
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SDS
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PBS.pdf
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Sodium Azide.pdf
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