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6X His tag antibody

Anti-6X His tag antibody used in Western Blot (WB). GTX30500

Cat No. GTX30500

Reference 1

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP, ELISA, IHC
Package
100 μl ($339)
See all 6X His tag products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:100- 1:500
ICC/IF 1:100-1:400
IP 1-4μg antibody/mg lysate
ELISA 1:1,000-1:30,000 (Detection) ; 1:100-1:500 (Coating)
IHC Assay dependent
Not tested in other applications.

Specificity/Sensitivity

This antibody does not recognize all N-terminal 6-His tagged proteins.

PROPERTIES

Form

Liquid

Buffer

PBS pH 7.2, 0.09% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

6-His (HHHHHH) conjugated to KLH.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_385678

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Research Area

DATA IMAGES

Anti-6X His tag antibody used in Western Blot (WB). GTX30500

GTX30500 WB Image

WB analysis of 200, 100, and 50ng of E. coli lysate containing tagged fusion protein using GTX30500 6X His tag antibody.
Dilution : 1:5000

REFERENCE

Application Reference

Zaki MS et al. Ann Neurol 2016; (Epub) PYCR2 mutations cause a lethal syndrome of microcephaly and failure to thrive.
Application : WB
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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339