GeneTex
  • Country / Location Selection

United States (US)

6X His tag antibody

Anti-6X His tag antibody used in Western Blot (WB). GTX30500

Cat No. GTX30500

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IP, ELISA, IHC
Package
100 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:100- 1:500
ICC/IF 1:100-1:400
IP 1-4μg antibody/mg lysate
ELISA 1:1,000-1:30,000 (Detection) ; 1:100-1:500 (Coating)
IHC Assay dependent
Not tested in other applications.

Specificity/Sensitivity

This antibody does not recognize all N-terminal 6-His tagged proteins.

PROPERTIES

Form

Liquid

Buffer

PBS pH 7.2, 0.09% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

6-His (HHHHHH) conjugated to KLH.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_385678

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

DATA IMAGES

Anti-6X His tag antibody used in Western Blot (WB). GTX30500

GTX30500 WB Image

WB analysis of 200, 100, and 50ng of E. coli lysate containing tagged fusion protein using GTX30500 6X His tag antibody.
Dilution : 1:5000

REFERENCE

REVIEW

There are currently no reviews for 6X His tag antibody (GTX30500). Be the first to share your experience with this product.
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339