GeneTex
United States (US)

6X His tag antibody

Cat No. GTX30500

Reference 1
Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, ELISA
See all 6X His tag products
APPLICATION

Application Note

For ELISA: (Coating) Use at a dilution of 1:100-1:500. (Primary) Use at a dilution of 1:1,000 - 1:30,000. For WB: (colorimetric) Use at a dilution of 1:1,000-1:10,000. (ECL) Use at a dilution of 1:1,000-1:30,000. For ICC: Use at a dilution of 1:200-1:2,000. Optimal dilutions/concentrations should be determined by the researcher.

Specificity/Sensitivity

Antibody recognizes 6-His (HHHHHH).
PROPERTIES

Form

Liquid

Buffer

PBS, pH 7.2, and 0.1% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Goat

Immunogen

Goats were immunized with 6-His (HHHHHH) peptide. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support.

Purification

Affinity purified with antigen

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

6X His epitope tag HHHHHH epitope tag HHHHHH tag

Background

This antibody recognises proteins containing the motif H-H-H-H-H-H and is therefore of value in detecting proteins containing histidine tags. The antibody may be used to track the purification of proteins expressed in bacteria, yeast or insect cells.

Research Area

REFERENCE

Application Reference

Zaki MS et al. Ann Neurol 2016; (Epub ahead of print) PYCR2 mutations cause a lethal syndrome of microcephaly and failure to thrive.
Application : WB
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Package List Price ($)
$ 239