For ELISA: (Coating) Use at a dilution of 1:100-1:500. (Primary) Use at a dilution of 1:1,000 - 1:30,000. For WB: (colorimetric) Use at a dilution of 1:1,000-1:10,000. (ECL) Use at a dilution of 1:1,000-1:30,000. For ICC: Use at a dilution of 1:200-1:2,000. Optimal dilutions/concentrations should be determined by the researcher.
Antibody recognizes 6-His (HHHHHH).
PBS, pH 7.2, and 0.1% sodium azide
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.
1mg/ml(Please refer to the vial label for the specific concentration.)
Goats were immunized with 6-His (HHHHHH) peptide. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support.
Affinity purified with antigen
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
6X His epitope tag HHHHHH epitope tag HHHHHH tag
This antibody recognises proteins containing the motif H-H-H-H-H-H and is therefore of value in detecting proteins containing histidine tags. The antibody may be used to track the purification of proteins expressed in bacteria, yeast or insect cells.