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6X His tag antibody

Anti-6X His tag antibody used in ELISA (ELISA). GTX77352
Anti-6X His tag antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX77352

Cat. No. GTX77352

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP, ELISA
Package
100 μg ($399)
See all 6X His tag products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.5 - 0.05 ug/ml
ICC/IF Assay dependent
IP 2 ug/mg
ELISA 2 ug/ml
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS pH7.2

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Rabbits were immunized with HHHHHH conjugated with KLH. After multiple immunizations in Freunds adjuvant serum was collected. Antibodies were immunoaffinity purified using the peptide immobilized on a solid support

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_379956

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-6X His tag antibody used in ELISA (ELISA). GTX77352

GTX77352 ELISA Image

ELISA analysis of immunogen using GTX77352 6X His tag antibody.

Anti-6X His tag antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX77352

GTX77352 ICC/IF Image

ICC/IF analysis of immunogen using GTX77352 6X His tag antibody.

REFERENCE

Application Reference

Bartolini E et al. Nat Commun 2020; 11 (1) : 4994 Immunological fingerprint of 4CMenB recombinant antigens via protein microarray reveals key immunosignatures correlating with bactericidal activity.
Moodley C et al. Anaerobe 2015; 32 : 23255 Molecular characterisation of ABC-type multidrug efflux systems in Bifidobacterium longum.
Application : WB
Submit a Reference

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399