6x His Tag antibody [GT636]

Cat. No. GTX636472

Host	Mouse
Clonality	Monoclonal
Clone Name	GT636
Isotype	IgG1
Application	WB, ICC/IF
Package	100 μl ($399), 25 μl ($169)

See all 6X His tag products

Datasheet

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:1000-1:10000
ICC/IF	Assay dependent

Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

1XPBS pH7, 20% Glycerol

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to 6X His tag

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

GTX636472 ICC/IF Image

6x His Tag antibody [GT636] detects 6x His-Tag tagged protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: 6x His-Tag tagged protein stained by 6x His Tag antibody [GT636] (GTX636472) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX636472 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with 6x His Tag antibody [GT636] (GTX636472) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty Custom Conjugations

6x His Tag antibody [GT636]

Cat. No. GTX636472

Host

Clonality

Clone Name

Isotype

Application