6x His Tag antibody [GT7712]

Cat. No. GTX636390

GTX636390 ICC/IF Image

6x His Tag antibody [GT7712] detects 6x His Tag protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: 6x His Tag stained by 6x His Tag antibody [GT7712] (GTX636390) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

1 / 3

GTX636390 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with 6x His Tag antibody [GT7712] (GTX636390) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

2 / 3

GTX636390 IP Image

Immunoprecipitation of 6x His-Tag tagged protein from 6x His-Tag tagged protein transfected 293T whole cell extracts using 5 μg of 6x His Tag antibody [GT7712] (GTX636390).
Western blot analysis was performed using 6x His Tag antibody [GT7712] (GTX636390).
EasyBlot HRP-conjugated anti mouse IgG antibody (GTX221667-01).

3 / 3

This 6x His Tag antibody [GT7712] (GTX636390) is a mouse monoclonal antibody validated for detection of 6x His Tag by WB, ICC/IF, and IP. Reactivity is species-independent.

Host

Mouse
Clonality

Monoclonal
Clone Name

GT7712
Isotype

IgG1
Applications

WB ICC/IF IP
Reactivity

Species independent