6x His Tag antibody [GT7712]

Cat. No. GTX636390

Host	Mouse
Clonality	Monoclonal
Clone Name	GT7712
Isotype	IgG1
Application	WB, ICC/IF, IP
Reactivity	Species independent
Package	100 μl ($399), 25 μl ($169)

See all 6X His tag products

Datasheet

View product citations for antibody GTX636390 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	Assay dependent
ICC/IF	Assay dependent
IP	1:100-1:1000

Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to 6X His tag.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

GTX636390 IP Image

Immunoprecipitation of 6x His-Tag tagged protein from 6x His-Tag tagged protein transfected 293T whole cell extracts using 5 μg of 6x His Tag antibody [GT7712] (GTX636390).
Western blot analysis was performed using 6x His Tag antibody [GT7712] (GTX636390).
EasyBlot HRP-conjugated anti mouse IgG antibody (GTX221667-01).

GTX636390 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with 6x His Tag antibody [GT7712] (GTX636390) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX636390 ICC/IF Image

6x His Tag antibody [GT7712] detects 6x His Tag protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: 6x His Tag stained by 6x His Tag antibody [GT7712] (GTX636390) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

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REVIEW

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Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty Custom Conjugations

6x His Tag antibody [GT7712]

Cat. No. GTX636390

Host

Clonality

Clone Name

Isotype

Application

Reactivity