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6x His Tag antibody [GT7712]

Anti-6x His Tag antibody [GT7712] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636390
Anti-6x His Tag antibody [GT7712] used in Immunoprecipitation (IP). GTX636390
Anti-6x His Tag antibody [GT7712] used in Western Blot (WB). GTX636390

Cat No. GTX636390

Host

Mouse

Clonality

Monoclonal

Clone Name

GT7712

Isotype

IgG1

Application

WB, ICC/IF, IP
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
IP 1:100-1:1000
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

1XPBS pH7, 20% Glycerol

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to 6X His tag.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

DATA IMAGES

Anti-6x His Tag antibody [GT7712] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636390

GTX636390 ICC/IF Image

6x His Tag antibody [GT7712] detects 6x His Tag protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: 6x His Tag stained by 6x His Tag antibody [GT7712] (GTX636390) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-6x His Tag antibody [GT7712] used in Immunoprecipitation (IP). GTX636390

GTX636390 IP Image

Immunoprecipitation of 6x His-Tag tagged protein from 6x His-Tag tagged protein transfected 293T whole cell extracts using 5 μg of 6x His Tag antibody [GT7712] (GTX636390).
Western blot analysis was performed using 6x His Tag antibody [GT7712] (GTX636390).
EasyBlot HRP-conjugated anti mouse IgG antibody (GTX221667-01).

Anti-6x His Tag antibody [GT7712] used in Western Blot (WB). GTX636390

GTX636390 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with 6x His Tag antibody [GT7712] (GTX636390) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 379
$ 169
Custom Conjugations