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8-Hydroxyguanosine antibody

Anti-8-Hydroxyguanosine antibody used in ELISA (ELISA). GTX00939

Cat No. GTX00939

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Application

IHC-P, ELISA
Package
100 μl ($369)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
IHC-P >1:200
ELISA Assay dependent

Note :

IHC-P
It is suggested that the tissues are pretreated with 10 μg/mL of proteinase K for 40 minutes at 37ºC prior to staining. Since the antibody is staining a nucleic acid it is important to use enough proteinase to penetrate the histone barrier.

Not tested in other applications.

Specificity/Sensitivity

By a competitive ELISA the antibody was 100% inhibited by 8-hydroxydeoxyguanosine and 8-hydroxyguanosine nucleotides. Partial cross-reactivity was observed with 8-mercaptoguanosine (80%) while 8-bromoguanosine showed some cross-reactivity (5%). 2-Deoxyadenosine, 7-methlyguanosine, guanosine monophosphate and guanosine showed no reactivity.

PROPERTIES

Form

Liquid

Buffer

Serum, 0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Immunogen

8-Hydroxy-2′-deoxyguanosine and 8-hydroxyguanosine

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

DATA IMAGES

Anti-8-Hydroxyguanosine antibody used in ELISA (ELISA). GTX00939

GTX00939 ELISA Image

Competitive ELISA analysis of nucleotide similar of 8-OHG using GTX00939 8-Hydroxyguanosine antibody. Partial cross-reactivity is observed with 8-mercaptoguanosine while 8-bromoguanosine shows some cross-reactivity. 2-Deoxyadenosine, 7-methylguanosine, guanosine monophosphate and guanosine show no reactivity.

REFERENCE

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REVIEW

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Package List Price ($)
$ 369