GeneTex
United States (US)

A-RAF antibody [C1C3]

GTX101427_IFA_19011506_601.jpg
GTX101427_WB_19011506_762.jpg
GTX101427_40079_IHC_19011506_407.jpg
GTX101427_WB_M_19011506_355.jpg

Cat No. GTX101427

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , NIH-3T3

Predict Reactivity

Rat, Zebrafish, Sheep, Bovine, Pig, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.85mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human A-RAF. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

A-Raf Proto-Oncogene, Serine/Threonine Kinase,A-Raf,Araf1,Pks2,Rafa1,Araf

Background

This proto-oncogene belongs to the RAF subfamily of the Ser/Thr protein kinase family, and maybe involved in cell growth and development. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101427_IFA_19011506_601.jpg

GTX101427 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using ARAF(GTX101427) antibody at 1:200 dilution.

GTX101427_WB_19011506_762.jpg

GTX101427 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
7.5% SDS PAGE
GTX101427 diluted at 1:1000

GTX101427_40079_IHC_19011506_407.jpg

GTX101427 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using ARAF(GTX101427) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101427_WB_M_19011506_355.jpg

GTX101427 WB Image

Sample (30 ug of whole cell lysate)

A:NIH-3T3
7.5% SDS PAGE

GTX101427 diluted at 1:1000

SDS
Glycerol.pdf
Thimerosal.pdf