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A-RAF antibody [C1C3]

Anti-A-RAF antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101427
Anti-A-RAF antibody [C1C3] used in Western Blot (WB). GTX101427
Anti-A-RAF antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX101427
Anti-A-RAF antibody [C1C3] used in Western Blot (WB). GTX101427

Cat No. GTX101427

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($319),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , NIH-3T3

Predict Reactivity

Rat, Zebrafish, Sheep, Bovine, Pig, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.85 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human A-RAF. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

A-Raf proto-oncogene, serine/threonine kinase , A-RAF , ARAF1 , PKS2 , RAFA1

Background

This proto-oncogene belongs to the RAF subfamily of the Ser/Thr protein kinase family, and maybe involved in cell growth and development. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-A-RAF antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101427

GTX101427 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using ARAF(GTX101427) antibody at 1:200 dilution.

Anti-A-RAF antibody [C1C3] used in Western Blot (WB). GTX101427

GTX101427 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
7.5% SDS PAGE
GTX101427 diluted at 1:1000

Anti-A-RAF antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX101427

GTX101427 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using ARAF(GTX101427) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-A-RAF antibody [C1C3] used in Western Blot (WB). GTX101427

GTX101427 WB Image

Sample (30 ug of whole cell lysate)

A:NIH-3T3
7.5% SDS PAGE

GTX101427 diluted at 1:1000

REFERENCE

There are currently no references for A-RAF antibody [C1C3] (GTX101427). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for A-RAF antibody [C1C3] (GTX101427). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169