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A549 whole cell lysate

A549 whole cell lysate. GTX27910

Cat No. GTX27910

Species

Human
Package
100 μg ($199)
PRODUCT

Summary

A549 lysate was prepared by homogenization in modified RIPA buffer. Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The Jurkat lysate was boiled for 5 min in 1 x SDS sample buffer containing 5% β-mercaptoethanol.

PROPERTIES

Form

Liquid

Buffer

1 x SDS sample buffer (50 mM Tris-HCl (pH 6.8), 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2 mg/ml (Please refer to the vial label for the specific concentration.)

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

DATA IMAGES

A549 whole cell lysate. GTX27910

GTX27910 Image

10μg of GTX109135-pro Galectin 2 recombinant protein analyzed using SDS-PAGE and stained with coomassie blue and captured by black and white camera.

REFERENCE

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Package List Price ($)
$ 199