GeneTex
United States (US)

AAK1 antibody, Internal

GTX88323_2419_WB_18121411_103.jpg

Cat No. GTX88323

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1-3μg/ml
Not tested in other applications.

Calculated MW

104 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88323-PEP).

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Concentration

0.50mg/ml

Antigen Species

Human

Immunogen

Peptide with sequence C-GSPRTSQQNVYNPSE, from the internal region of the protein sequence according to NP_055726.3.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Cellular Localization

Active when found in clathrin-coated pits at the plasma membrane.

Background

Adaptor-related protein complex 2 (AP-2 complexes) functions during receptor-mediated endocytosis to trigger clathrin assembly, interact with membrane-bound receptors, and recruit encodytic accessory factors. This gene encodes a member of the SNF1 subfamily of Ser/Thr protein kinases. The protein interacts with and phosphorylates a subunit of the AP-2 complex, which promotes binding of AP-2 to sorting signals found in membrane-bound receptors and subsequent receptor endocytosis. Its kinase activity is stimulated by clathrin. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]
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Database

Research Area

DATA IMAGES
GTX88323_2419_WB_18121411_103.jpg

GTX88323 WB Image

WB analysis of human amygdala lysate using GTX88323 AAK1 antibody, Internal.
Dilution : 1µg/ml
Loading : 35µg protein in RIPA buffer