*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
The peptide used to generate this antibody is available for purchase (GTX88323-PEP).
Mouse, Rat, Bovine(>80% identity)
Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.
Peptide with sequence C-GSPRTSQQNVYNPSE, from the internal region of the protein sequence according to NP_055726.3.
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Active when found in clathrin-coated pits at the plasma membrane.
Adaptor-related protein complex 2 (AP-2 complexes) functions during receptor-mediated endocytosis to trigger clathrin assembly, interact with membrane-bound receptors, and recruit encodytic accessory factors. This gene encodes a member of the SNF1 subfamily of Ser/Thr protein kinases. The protein interacts with and phosphorylates a subunit of the AP-2 complex, which promotes binding of AP-2 to sorting signals found in membrane-bound receptors and subsequent receptor endocytosis. Its kinase activity is stimulated by clathrin. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]
GTX88323 WB Image
WB analysis of human amygdala lysate using GTX88323 AAK1 antibody, Internal.
Dilution : 1µg/ml
Loading : 35µg protein in RIPA buffer