GeneTex
United States (US)

AAK1 antibody

GTX17021_2332_WB_20180221_18121410_709.jpg

Cat No. GTX17021

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ELISA
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 - 2 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

104 kDa. ( Note )

Positive Control

A20 Cell Lysate(GTX27180)
PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Aak1 antibody was raised against a 18 amino acid synthetic peptide near the carboxy terminus of the human Aak1.The immunogen is located within amino acids 830 - 880 of Aak1.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Ap2 Associated Kinase 1,Aak1

Background

Adaptor-related protein complex 2 (AP-2 complexes) functions during receptor-mediated endocytosis to trigger clathrin assembly, interact with membrane-bound receptors, and recruit encodytic accessory factors. This gene encodes a member of the SNF1 subfamily of Ser/Thr protein kinases. The protein interacts with and phosphorylates a subunit of the AP-2 complex, which promotes binding of AP-2 to sorting signals found in membrane-bound receptors and subsequent receptor endocytosis. Its kinase activity is stimulated by clathrin. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX17021_2332_WB_20180221_18121410_709.jpg

GTX17021 WB Image

WB analysis of A-20 cell lysate using GTX17021 AAK1 antibody.
Working concentration : (A) 1 and (B) 2 μg/ml

Package List Price ($)
$ 329