GeneTex
United States (US)

AAK1 antibody

GTX17023_2333_WB_20180221_18121410_159.jpg
GTX17023_724_IHC-P_20180221_18121410_967.jpg
GTX17023_40_ICC-IF_20180221_18121410_371.jpg

Cat No. GTX17023

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, ELISA
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 - 2 μg/mL
ICC/IF 5 μg/mL
IHC-P Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

104 kDa. ( Note )

Positive Control

Human Brain Tissue Slide(GTX22203) , A-20 Cell Slide(GTX25541) , Rat Spleen Tissue Lysate(GTX27945)
PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Aak1 antibody was raised against a 20 amino acid synthetic peptide near the amino terminus of the human Aak1.The immunogen is located within amino acids 190 - 240 of Aak1.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Ap2 Associated Kinase 1,Aak1

Background

Adaptor-related protein complex 2 (AP-2 complexes) functions during receptor-mediated endocytosis to trigger clathrin assembly, interact with membrane-bound receptors, and recruit encodytic accessory factors. This gene encodes a member of the SNF1 subfamily of Ser/Thr protein kinases. The protein interacts with and phosphorylates a subunit of the AP-2 complex, which promotes binding of AP-2 to sorting signals found in membrane-bound receptors and subsequent receptor endocytosis. Its kinase activity is stimulated by clathrin. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX17023_2333_WB_20180221_18121410_159.jpg

GTX17023 WB Image

WB analysis of A-20 cell lysate using GTX17023 AAK1 antibody.
Working concentration : (A) 1 and (B) 2 μg/ml

GTX17023_724_IHC-P_20180221_18121410_967.jpg

GTX17023 IHC-P Image

IHC-P analysis of human brain tissue using GTX17023 AAK1 antibody.
Working concentration : 20 μg/ml

GTX17023_40_ICC-IF_20180221_18121410_371.jpg

GTX17023 ICC/IF Image

ICC/IF analysis of A-20 cells using GTX17023 AAK1 antibody.
Working concentration : 5 μg/ml

Package List Price ($)
$ 329