GeneTex
United States (US)

ABAT antibody

GTX100479_39456_20170214_IHC-Fr_19011506_492.jpg
GTX100479_39456_WB_19011506_429.jpg
GTX100479_39456_IHC_M_2_19011506_579.jpg
GTX100479_39456_20160309_IHC-P_R_19011506_771.jpg
GTX100479_39456_20161005_IHC-Fr_R_19011506_328.jpg
GTX100479_39456_20161020_WB_M_R_19011506_129.jpg
GTX100479_39456_20160602_IFA_19011506_195.jpg
GTX100479_39456_IHC_M_19011506_549.jpg

Cat No. GTX100479

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

56 kDa. ( Note )

Positive Control

SK-N-SH , mouse brain , rat brain

Predict Reactivity

Bovine, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ABAT. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

4-Aminobutyrate Aminotransferase,Gaba-At,Gabat,Npd009,Abat

Cellular Localization

Mitochondrion matrix

Background

4-aminobutyrate aminotransferase (ABAT) is responsible for catabolism of gamma-aminobutyric acid (GABA), an important, mostly inhibitory neurotransmitter in the central nervous system, into succinic semialdehyde. The active enzyme is a homodimer of 50-kD subunits complexed to pyridoxal-5-phosphate. The protein sequence is over 95% similar to the pig protein. GABA is estimated to be present in nearly one-third of human synapses. ABAT in liver and brain is controlled by 2 codominant alleles with a frequency in a Caucasian population of 0.56 and 0.44. The ABAT deficiency phenotype includes psychomotor retardation, hypotonia, hyperreflexia, lethargy, refractory seizures, and EEG abnormalities. Multiple alternatively spliced transcript variants encoding the same protein isoform have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100479_39456_20170214_IHC-Fr_19011506_492.jpg

GTX100479 IHC-Fr Image

ABAT antibody detects ABAT protein expression by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: ABAT protein stained by ABAT antibody (GTX100479) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

GTX100479_39456_WB_19011506_429.jpg

GTX100479 WB Image

ABAT antibody detects ABAT protein by western blot analysis.
A. 30 µg SK-N-SH whole cell lysate/extract
7.5% SDS-PAGE
ABAT antibody (GTX100479) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100479_39456_IHC_M_2_19011506_579.jpg

GTX100479 IHC-P Image

ABAT antibody detects ABAT protein at mitochondria on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
ABAT antibody (GTX100479) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100479_39456_20160309_IHC-P_R_19011506_771.jpg

GTX100479 IHC-P Image

ABAT antibody detects ABAT protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
ABAT antibody (GTX100479) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX100479_39456_20161005_IHC-Fr_R_19011506_328.jpg

GTX100479 IHC-Fr Image

ABAT antibody detects ABAT protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: ABAT protein stained by ABAT antibody (GTX100479) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX100479_39456_20161020_WB_M_R_19011506_129.jpg

GTX100479 WB Image

Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with ABAT antibody (GTX100479) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100479_39456_20160602_IFA_19011506_195.jpg

GTX100479 ICC/IF Image

ABAT antibody detects ABAT protein at mitochondria by immunofluorescent analysis.
Sample: HepG2 cells were fixed in ice-cold MeOH for 5 min.
Green: ABAT protein stained by ABAT antibody (GTX100479) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX100479_39456_IHC_M_19011506_549.jpg

GTX100479 IHC-P Image

ABAT antibody detects ABAT protein at mitochondria on mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
ABAT antibody (GTX100479) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf