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ABAT antibody

Anti-ABAT antibody used in Western Blot (WB). GTX101413
Anti-ABAT antibody used in Western Blot (WB). GTX101413
Anti-ABAT antibody used in IHC (Paraffin sections) (IHC-P). GTX101413
Anti-ABAT antibody used in IHC (Paraffin sections) (IHC-P). GTX101413
Anti-ABAT antibody used in Western Blot (WB). GTX101413

Cat No. GTX101413

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
See all ABAT products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

56 kDa. ( Note )

Positive Control

SK-N-SH , mouse brain , rat liver

Predict Reactivity

Bovine, Pig, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.49 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human ABAT. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

4-aminobutyrate aminotransferase , GABA-AT , GABAT , NPD009

Cellular Localization

Mitochondrion matrix

Background

4-aminobutyrate aminotransferase (ABAT) is responsible for catabolism of gamma-aminobutyric acid (GABA), an important, mostly inhibitory neurotransmitter in the central nervous system, into succinic semialdehyde. The active enzyme is a homodimer of 50-kD subunits complexed to pyridoxal-5-phosphate. The protein sequence is over 95% similar to the pig protein. GABA is estimated to be present in nearly one-third of human synapses. ABAT in liver and brain is controlled by 2 codominant alleles with a frequency in a Caucasian population of 0.56 and 0.44. The ABAT deficiency phenotype includes psychomotor retardation, hypotonia, hyperreflexia, lethargy, refractory seizures, and EEG abnormalities. Multiple alternatively spliced transcript variants encoding the same protein isoform have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ABAT antibody used in Western Blot (WB). GTX101413

GTX101413 WB Image

ABAT antibody detects ABAT protein by Western blot analysis.
A. 50 μg Rat liver lysate/extract
7.5 % SDS-PAGE
ABAT antibody (GTX101413) dilution: 1:1000

Anti-ABAT antibody used in Western Blot (WB). GTX101413

GTX101413 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX101413 diluted at 1:1000

Anti-ABAT antibody used in IHC (Paraffin sections) (IHC-P). GTX101413

GTX101413 IHC-P Image

ABAT antibody detects ABAT protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
ABAT antibody (GTX101413) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ABAT antibody used in IHC (Paraffin sections) (IHC-P). GTX101413

GTX101413 IHC-P Image

ABAT antibody detects ABAT protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
ABAT antibody (GTX101413) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ABAT antibody used in Western Blot (WB). GTX101413

GTX101413 WB Image

ABAT antibody detects ABAT protein by Western blot analysis.
A. 30 μg HepG2 whole cell lysate/extract
7.5 % SDS-PAGE
ABAT antibody (GTX101413) dilution: 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169