APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
SK-N-SH , mouse brain , rat liver
Predict Reactivity
Bovine, Pig, Xenopus laevis(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.49 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human ABAT. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
4-aminobutyrate aminotransferase , GABA-AT , GABAT , NPD009
Cellular Localization
Mitochondrion matrix
Background
4-aminobutyrate aminotransferase (ABAT) is responsible for catabolism of gamma-aminobutyric acid (GABA), an important, mostly inhibitory neurotransmitter in the central nervous system, into succinic semialdehyde. The active enzyme is a homodimer of 50-kD subunits complexed to pyridoxal-5-phosphate. The protein sequence is over 95% similar to the pig protein. GABA is estimated to be present in nearly one-third of human synapses. ABAT in liver and brain is controlled by 2 codominant alleles with a frequency in a Caucasian population of 0.56 and 0.44. The ABAT deficiency phenotype includes psychomotor retardation, hypotonia, hyperreflexia, lethargy, refractory seizures, and EEG abnormalities. Multiple alternatively spliced transcript variants encoding the same protein isoform have been found for this gene. [provided by RefSeq]
Database
Research Area
DATA IMAGES
|
GTX101413 WB Image
ABAT antibody detects ABAT protein by Western blot analysis.
A. 50 μg Rat liver lysate/extract
7.5 % SDS-PAGE
ABAT antibody (GTX101413) dilution: 1:1000
|
|
GTX101413 WB Image
Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX101413 diluted at 1:1000
|
|
GTX101413 IHC-P Image
ABAT antibody detects ABAT protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
ABAT antibody (GTX101413) diluted at 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
|
|
GTX101413 IHC-P Image
ABAT antibody detects ABAT protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
ABAT antibody (GTX101413) diluted at 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
|
|
GTX101413 WB Image
ABAT antibody detects ABAT protein by Western blot analysis.
A. 30 μg HepG2 whole cell lysate/extract
7.5 % SDS-PAGE
ABAT antibody (GTX101413) dilution: 1:1000
|
REFERENCE
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REVIEW
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