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ABCA1 antibody

Anti-ABCA1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132946
Anti-ABCA1 antibody used in Western Blot (WB). GTX132946

Cat. No. GTX132946

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all ABCA1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

254 kDa. ( Note )

Predict Reactivity

Mouse, Rat, Bovine, Chicken, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.34 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human ABCA1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886780

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ATP binding cassette subfamily A member 1 , ABC-1 , ABC1 , CERP , HDLDT1 , TGD

Cellular Localization

Membrane

Background

The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intracellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ABC1 subfamily. Members of the ABC1 subfamily comprise the only major ABC subfamily found exclusively in multicellular eukaryotes. With cholesterol as its substrate, this protein functions as a cholesteral efflux pump in the cellular lipid removal pathway. Mutations in this gene have been associated with Tangier's disease and familial high-density lipoprotein deficiency. [provided by RefSeq, Jul 2008]

Database

  • Gene ID: 19 ABCA1
  • UniProt: O95477 ABCA1

Research Area

DATA IMAGES

Anti-ABCA1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132946

GTX132946 ICC/IF Image

ABCA1 antibody detects ABCA1 protein at cytoplasm by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ABCA1 stained by ABCA1 antibody (GTX132946) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10 μm.

Anti-ABCA1 antibody used in Western Blot (WB). GTX132946

GTX132946 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with ABCA1 antibody (GTX132946) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Taisuke Okano et al. International Journal of Endocrinology 2020; 2020 : Article ID 4139093 β-Endorphin Mediates the Development and Instability of Atherosclerotic Plaques
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169